High performance team

Pharmacopoeial and non-Pharmacopoeial specifications. Dissolution testing was undertaken using USP Apparatus 2 (Paddle Type), which allowed for a more realistic assessment and prediction of in vitro drug release rates. Samples were analysed using a high performance liquid chromatographic method (HPLC). Formulation F5 shows optimum drug release. Drug and rate retarding polymers ratio used in this formulation were Methocel K100 LV (14.86% w/w) and Methocel K4M (10.14%w/w), in ratio (5.4:1.34:1). The results

and benzoic acid are common additives to beverages for the stimulation effect and as a preservative, respectively. To simultaneously determine the amount of each of these chemicals, a method combining UV/Visible spectroscopy and reversed-phase high performance liquid chromatography was introduced. The experimentally determined concentrations at the 95% confidence interval of caffeine and benzoic acid in Mountain Dew were 149 +/- 5 ppm and 308 +/- 6 ppm, respectively. The method showed separation of

Metabolomics: A field of life science research that uses high throughput (HT) technologies to identify and or characterized all the small molecules or metabolites in a given cell, tissue or organ (I.e. the metabolite). Metabolite: Any natural atom discernible in the body with the sub-atomic weight (MW) under 1500 Dalton's. It incorporates liveliness tides, oligonucleotides, sugars, nucleosides, natural acids, ketones, aldehydes, amines, amino acids, steroids, alkaloids, nourishment added substances

yields high performance and high speed compared with traditional column chromatography because of the forcibly pumped mobile phase. Recently, ultrafast analysis using a high-pressure-resistant apparatus has been attracting attention. UHPLC (Ultra High Performance LC) is becoming established as an abbreviation for this ultrafast LC method. In 1941 Martin and Synge, described the discovery of liquid-liquid partition chromatography and also laid the foundation of Gas liquid chromatography and High performance

Lab Report 4: Protein Purification: Size Exclusion Chromatography Badrun Nessa Rahman Lab Partner: Briana Tolbert Section 55 Objective: The purpose of today’s laboratory is to understand how size exclusion chromatography plays a role in purifying protein which is an important concept to understand as it helps answer the mysteries which lay behind proteins such as

(1975). HPLC uses either a solid or liquid-coated solid stationary phase and a liquid mobile phase. Separation of analytes is achieved by adsorption of the analyte to the stationary phase. HPLC provides speed, resolution, high sensitivity and specificity (Lima, 2002). HPLC also be a lower temperature than GC methods; reducing the risk of isomerization of unsaturated fatty acids. HPLC has an immediate advantage over TLC, because reduces the exposure of the sample to atmospheric

Sophie Tran Chem 15 Prof. Neumark Nov 30. 2016 Partner: Megumi Mori GSI: Erin Experiment #8: Quantitative Analysis of a Solution Containing Cobalt and Copper I. Abstract: II. Introduction: This experiment will help students to get familiar with Ion Exchange Chromatography (IEC) which is used mostly to separate unknown mixture of metal ions. Anion and cation are known as two types of IEC. This column chromatography contains two phase such as stationary phase and mobile phase. In this experiment

In this lab, paper chromatography will be used to separate the components of known and unknown mixtures and then used to identify those components. Paper chromatography is a technique where a drop of solution containing a mixture will be placed on a piece of filter paper. One end of the filter paper will then be placed into a liquid solvent. The mixture will separate into its different components as the solvent moves up the filter paper. The filter paper is known as the stationary phase. The solvent

Figure 1(a)(b) represent a set of data that shows the relationship of pink/gold melanin being absorbed at 475nm versus time (min). Figure a and b should be combined, but for clarity purposes were separated. In figure 1a, it contains three deceleration curves. The deceleration curve containing the blue diamonds represents the data points of the direct proportion. There are 8 points that are slightly touching the line of best fit. There are 5 points intersecting the deceleration curve. Around 13-20mins

Abstract A simple, rapid, sensitive and effective reverse phase ultra performance liquid chromatographic method (RP-UPLC) has been developed and validated an assay for simultaneous quantification of Lamivudine, Abacavir and Dolutegravir in pure and tablet dosage forms. Chromatographic separation was performed by using Waters- Alliance UPLC system equipped with autosampler, PDA detector, zodiac sil RP C18 (4.6×250mm 3.0µm) column, phosphate buffer (pH 3.0) and methanol in the ratio of 30:70 v/v have