Molecular biology

a large number of species of microbes found on the human body. This bacterial organism are found in the skin, mouth, or nose. This lab consisted of the collection of skin bacterial organisms and amplification of the 16s rRNA to construct a small molecular phylogeny of the human body microbiome, or the community of microorganisms that reside in the epithelia of humans. This information could only be acquired through processes such as DNA extraction, amplification of specific genetic target by the polymerase

(AGE) is the most common and effective way of separating and analyzing DNA fragments of different sizes (Lee & Costumbrado, 2012). The agarose gel, at the time that it is completely polymerized, acts as a molecular sieve that, in the existence of electric current, pull apart DNA by their molecular weight and size. AGE is not mostly used to visualize DNA but also used for quantification or to single out a particular band. The DNA is visualized in the gel by staining with of ethidium bromide (EtBr).

recombination reaction, this cassette is replaced by the gene of interest to generate the entry clone and expression clone, respectively. 6) Gel Electrophoresis Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of DNA or proteins in a matrix ofagarose. The proteins may be separated by charge and/or size

My aunt was trying to cope with the situation while devastation, guilt, frustration, and grief weighed her down. During this time, I was attending my second year in high school and learned as much about genes and genetic diseases as a high school biology course would let me. Even so, I held on to what little knowledge I had of genetics and gave much thought to the baby I shared genes with who suffered from an extra chromosome. In the course of time, the investment I made in understanding my baby cousin’s

Transformation in bacteria is something that could be essential for survival in a bacteria. In order to perform this transformation naturally a bacterium must considered competent, otherwise it must undergo an artificial transformation. Being a competent cell means that the bacteria can take up DNA from its environment naturally (5). Those that are not competent such as Escherichia coli that are not naturally competent can be tested with an artificial transformation, such as what we will use in

molecules such as proteins worked together as a complex system, and how spatial cues were being controlled. Dr. Gitai later joined Princeton University’s faculty and became the Director of Graduate Studies for the Department of Molecular Biology. Now Dr. Gitai focuses on the cell biology of bacteria and how their cells are able to self-organize across spatial scales. In the seminar that Dr. Gitai gave, he presented several questions that he asked himself, and his research team, while investigating why bacteria

temperature specified for the restriction enzyme(s). In order to determine where the enzyme(s) cut on the plasmid and how many times the enzyme(s) made a cut, gel electrophoresis is employed. Restriction enzymes are important for many aspects of molecular biology such as cloning and restriction mapping. The enzymes are also useful since there are a lot of restriction enzymes that cut the DNA at different locations. The importance of different enzymes cutting different sites makes it possible for scientists

genetic information to produce proteins? Intro(10mins) Bacteria belongs to a group of organism that lacks cell nucleus and membrane bound organells. This group of organisms are termed as prokaryotes. Prokaryotes follows the central dogma of molecular biology first proposed by Francis Crick in 1958 to synthesize proteins from mRNA through a process called translation and the mRNA is being synthesized from the DNA by another process called Transcription. Temperature, nutrient availibity are some key

implications in histone acetyltransferase activity. Introduction: Research is a slow and meticulous process required to understand the smallest details of the universe. That is why, in order to study an organism, we must delve deep into their molecular biology. With that said, to understand the processes of the common fruit fly, Drosophila melanogaster, we must look at their genome to which their function comes from. So with the discovery a new gene, we must be able to isolate, amplify, purify, and

Eunice Assibu Lab Partners: Sage Gibson and Vanessa Song. TA: Mian Li Undergrad TA: Haley linnet burdge Bio 118-A60 November 10, 2015 Genetic Transformation Introduction There were two parts of this lab and part 1 was; Transforming E. coli with the pGreen plasmid and Part 2; PCR and Electrophoresis. For this lab, a genetic transformation procedure was performed to introduce a plasmid to another cell and when the cell reproduces it will make a new copy of the plasmid. And genetic transformation