Molecular biology

Introduction Forensic DNA Phenotyping is a new and emerging field of forensic science. As it is so new, there is very little in terms of literature on this field, very little testing done on this type of identification, and few cases that have used DNA Phenotyping to assist in the investigation. The first documented case of DNA Phenotyping being used is in the early 2000s, so it has had under 20 years of research and experimentation with it. There are many weaknesses to the version of DNA Phenotyping

Recombinant DNA technology is genetic engineering process for forming a new gene. The gene required is taken from the donor and joined with the carrier gene which is then inserted into the vector. This method is used to create a vector containing gene from Bacteria sp Yp1 and Esterobacter asburae YT1, which are then inserted to egg through microinjection. Microinjection is a process where screw held syringe is loaded with required DNA or RNA and inserted to animal cell. By this technique the cloned

1.1. Name of target and key citation 0% Beta Catenin (β-catenin) or Catenin Beta-1 (CTNNB1) protein. Key citation: Ma, X.Y., Ma, C.X. and Wang, J.H. (2014) Endometrial Carcinogenesis and Molecular Signaling Pathways. American Journal of Molecular Biology, 4, 134-149. 1.2. What is the normal function of the target? 10% β-catenin is a proto-oncogene and it is encoded by CTNNB1 gene. It forms a component of the E-cadherin – catenin unit which plays essential roles in the cell differentiation

a UV transilluminator. Negatively charged DNA will move towards the positively charged anode through the agarose gel, while the migration of DNA is dependent of molecular DNA size, agarose concentration, DNA conformation and applied current. Plasmid DNA extracted from bacterial cells can exist in three different conformations of molecular size and will migrate at different rates. The mobility of these forms will be influenced by agarose concentration and the strength of applied

categorized into two distinct populations based on the TCR gene usage and antigen specificity, namely the invariant/type I NKT TCRs and diverse/type II NKT TCRs (Godfrey et al., 2004b). For the past two decades, much of the work in the field of NKT biology focused on the type I NKT cells largely due to their ability to recognize α-GalCer loaded CD1d tetramers (Benlagha et al., 2000). While CD1d-α-GalCer tetramers still remain as a major tool to characterize NKT cells, type II NKT cells do not recognize

I’m currently a molecular biology major, and so far out of all the science courses I have taken I loved genetics the most. I always had a fascination in genetics because the science of heredity is so complex and based largely off of probability. A recent technology that has been created in this field is called CRISPR, which is a gene editing technique that allows unfavorable pieces of genomic DNA to be cut out. This is a controversial topic, as some people are against the idea of having scientists

Denise Rendon Mr.pinto Biology H 4-15-16 Francis Crick Francis Crick was a Biophysic born in Northampton, England(1916). He helped create new technologies during World War II.After the war, he began investigating the structure of DNA with the Research Council of the University of Cambridge Medical at its Cavendish Laboratory with James D. Watson. He received a Nobel Prize for Physiology or Medicine in 1962 which he shared for his work and he continued coordinating investigation until 2004 when

Charlene Forest is an associate professor in the Biology department at Brooklyn College, who dedicates her research in to trying to understand the mechanism behind the process of fertilization in algae, as well as what controls expression of gamete-specific genes. To do so, she must understand how sperm and egg gametes first recognize and then fuse with each other. Thus, in order to find what causes the fusion of these gametes, Forest’s lab is cloning genes that prevent the fusion of sperm and egg

8. DNA purification -DNA purification is an important method that involved in many areas of molecular biology, genomics, biotechnology and clinical research. Purification will reduce the chance of contaminations happen during the experiments and extract amount of DNA sample from limited source to satisfy the requirements of research. The whole procedure

has application in molecular genetics & genetic fingerprinting of PCR products. • It is used to separate genomic DNS (Southern Blot) and RNA (Northern Blot). • It’s used to resolve circular DNA with different supercoiling topology and to resolve fragments that differ due to DNA synthesis. The is reduction of DNA damage due to cross-linking proportionally with electrophoretic DNA migration. • Agarose gel aids purification of DNA fragments which is necessary for a number molecular techniques such