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    Wistar Preparation Report

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    Before proceeding with experimentation, the researchers had their procedure approved by a local animal experiment committee. The researchers proposed procedures followed all the national and institutional guidelines that the animal experiment committee enforces. Preparation Organ Explant Cultures. The researchers used two-day old Wistar rats to obtain an organ explant culture. The head of the Wistar rat was decapitated and the heart was removed and stored in a Hank's Balanced Salt Solution (HBSS)

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    Red Blood Cells

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    Red blood cell From Wikipedia, the free encyclopedia [pic] Human red blood cells (6-8μm) Red blood cells (also referred to as erythrocytes) are the most common type of blood cell and the vertebrateorganism's principal means of delivering oxygen (O2) to the body tissues via the blood flow through thecirculatory system. They take up oxygen in the lungs or gills and release it while squeezing through the body'scapillaries. These cells' cytoplasm is rich in hemoglobin, an iron-containing biomolecule that

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    Claudins are the specific proteins that control paracellular movement through the formation of a barrier in the epithlelial monolayer. Thus, claudins are also the proteins that form the water channels needed to facilitate transportation across the

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    Introduction and Rationale for Conducting the Research - Include in this section a brief review of pertinent literature and any available preliminary data. (1 page maximum) In the United States, salmonellosis is the second most common foodborne disease, with 800,000 to 4 million cases occurring each year with over 300, 000 hospitalization and approximately 500 deaths (4,13). Salmonella enterica serover Typhimurium colonize in the intestinal tracts of many mammals and birds, and one of the major routes

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    Experimental Methods to Measure the Contact Angle of Particles at Fluid Interfaces Name: Caiyi Mai Student Number: 250821954 Department: Chemical and Biochemical Engineering   Abstract Wettability of the particles plays an important role and is reflected by the three-phase contact angle, θ. However, the contact angle is frequently neglected in the studies. This mini review focused on the experimental methods that use to measure the contact angle of particles at fluid interface. The methods

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    Top-down methods fabrications Thin flakes of TMDs can be peeled off from bulk materials using adhesive tape, applied to the substrates and then identified by light interference using similar techniques used to develop graphene. Fig 6c shows a thin monolayer flake peeled off from the bulk material (Fig 6a) mechanically with the tape. Oxide nanosheets as well as other materials can be obtained using this method. Using the mechanical method of exfoliation helps to produce flakes of high purity that

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    Wound Healing Assay

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    A cell suspension containing 0.5 -1.0 x 10^6 cells/ml in media 10% FBS (Fetal Bovine Serum) was placed on a 24- well plate so a monolayer can be created at the bottom of the wells. The first step will consist of the lab student aspirating the media from the wells making sure to disrupt the monolayer of cells at the bottom of the wells. Next the wells will be washed with PBS to remove dead cells and debris. After the wells are washed 1ml of media (DMEM) in a 5%

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    formation of a narrow neck are consequences of line tension and the need to reduce the length of the energetically unfavourable domain boundary. Deformation of the lipid molecules in opposing monolayers is present, with lipids in the outer monolayer adopting an extended conformation, whereas those in the inner monolayer become compressed and splayed. Thinning at the neck is driven by the formation of lipid microdomains and the accumulation of lipids with negative spontaneous curvature in the most deformed

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    Pigment Epithelium (RPE)

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    elderly population, which was estimated to affect around 200 million people worldwide in 2020[1] and places vast socioeconomic burden. Degeneration of retinal pigment epithelium (RPE) is believed to play central roles in AMD pathogenesis. RPE is a monolayer of highly pigmented cuboidal cells residing between the photoreceptors (PRs) and Bruch’s membrane. One RPE cell serves around thirty photoreceptors in terms of supporting the visual cycle and phagocytosis of outer segments[2]. As a layer of epithelium

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    In vitro results showed that UV-C treatment not only improved the cell spreading and attachment, but also it made the cells more metabolically active. UV-C effectively removed the hydrocarbon contaminants and exposed the TiO2 on the surface, which is a good indication for higher and faster bone-to-implant contact than hydrocarbon-coated implants. Larsson et al. (1996) showed that high degrees of bone contact and bone formation were achieved with titanium implants which were modified on oxide thickness

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