Phage display

Phage display is a modern laboratory technique that is concerned with the study of not only protein-protein interactions but also protein-peptide and protein-DNA interactions through the use of a bacteriophage. Phage display uses a bacteriophage or a virus that infects bacteria to connect phenotype with genotype through the expression of a gene. This novel technique first starts by inserting a gene of interest, which codes for a protein of interest, into the coat protein gene of the virus, thereby

Construction of the nanobody library Overlapping PCR method was performed for the construction of CDR3 region from degenerate primer (Fig.2a). Degenerate primer in CDR3, according to NKK number in primer, determined diversity of the library. The diversity of the library theoretically could be 10 24, but practically it was estimated to be 5×1011CFU/ml. By PCR assembly approach, CDR3 region was linked to the framework using primer listed in table1 (Fig.2b). In order to obtain acceptable diversity,

Before the age 20, I had already been to three different countries and many cities. Which allows me to meet with different people, experience different cultures and encounter different believes. I was born in Mundemba, a small town in the South West region of Cameroon. Cameroon is a located in the western part of central Africa. Life in Cameroon was simple and fun. I spent the first nineteen years of my life there during which I completed high school. After developing and immense love for the sciences

virus will cause damage to their intestines because that is solely what cholera targets, and the purpose of the phages are to combat the cholera bacteria, which would in turn help their intestines. 5. The release of a toxin from the cholera bacteria is what causes people to experience the dreadful symptoms. A negative health concern of killing the cholera at a quick rate is that since the phages will prevent symptoms from increasingly becoming worse, the “dead” cholera in the intestine may sit there for

with the first investigation into the phenomenon of phages are Frederick Twort, a medically trained bacteriologist from England, and Felix d’Herelle, a French-Canadian microbiologist. The history of phage discovery and research is so complicated that the scientific world has simply accepted referring to the discovery as the “Twort-d’Herelle phenomenon.” Twort was the first to notice and describe the characteristics of lysis associated with phage infection in 1915. However, Abedon states that it was

Every epic hero possesses certain heroic characteristics. The epic poem Beowulf describes the most heroic man of the Anglo-Saxon times. Beowulf is the hero. He shows that he is a great man by always putting other things before his own needs. He is important and needed by his people and is known by many as a strong, courageous and a helpful person. He shows all of the qualities and traits that a true hero possesses. Beowulf, like other epic heroes, possesses the following heroic qualities: epic

A Planet of Viruses For centuries new viruses have been discovered each and everyday by thousands of different scientists. These viruses affect the lives of almost a billion people. Even though many of these viruses can be harmful to the human body, they have played a phenomenal role in the biological advances over the past century. They move DNA between species, regulate vast populations of organisms, and provide new genetic material for evolution. Viruses influence every single species on earth

When I was young, I came to understand that a person’s creativity cannot be constrained to their situation. I learned that everything we do is based off the choices in our life. While someone can offend, discourage or harm you, they cannot force your response. As a decision maker, this taught me a lot about how I need to approach choices; that is, I cannot rely solely on instinct to choose a correct path. Mentors, friends, and family are needed to guide decisions, because they will weigh options

1.5.1.3 R-type pyocin: Therapeutic potential The potential of R-type pyocin to be developed as a therapeutic was realized immediately after its discovery. As early as in 1967, Higerd et al showed that R-type pyocin was safe to administer in animals [77]; it did not have a lethal effect on mice and rabbits when injected in high concentration intraperitoneally. Since then, six studies have specifically looked at the therapeutic potential of R-type pyocins in animal models. Bird and Grieble, in 1969

Bacteriophages (or in short, Phages) are viruses that solely infect bacteria, in fact, each Phage can only infect a very specific type of bacteria in order to survive and replicate. like other viruses, Phages need a bacterial host in order to replicate their viral DNA , RNA etc… Phages are also named after the strain of bacteria which they infect eg: coli phages infect the bacteria E-coli. each Phage can vary in shape and size but they all contain Capsids (protein coats) which surround their viral