Sucrose solution

monitoring an enzyme-catalysed reaction sequence involving the appearance of NADPH. Sucrose and glucose concentrations were calculated from the concentration of NADPH formed by the reaction of glucose-6-phosphate and NADP+. Spectrophotometric absorbance readings were taken at 340nm, this is because NADPH absorbs strongly at this wavelength, whilst NADP+ does not (1015MSC, 2010). The concentration of glucose and sucrose in

the pH of a substance affects the rate at which the enzyme catalyses sucrose. Enzymes are biological catalysts that are protein molecules. As catalysts, they work to affect the rate of chemical reactions. Catalysts do this without being changed by the reaction or changing the reaction itself. Enzymes are substrate specific meaning they can only catalyze one specific substrate. In the experiment at hand, the substrate, sucrose, is hydrolyzed with sucrase to produce glucose and fructose. The enzyme

Carbohydrate Analysis Introduction and Purpose Carbohydrates are organic compounds that consist of carbon, oxygen, and hydrogen. There are four different ways that carbohydrates can be classified: monosaccharides, oligosaccharides, and polysaccharides. Monosaccharides are the simplest sugars. They are aliphatic aldehydes or ketones and most have five or six carbon atoms. Oligosaccharides are two monosaccharides linked together by the elimination of a water molecule which allows the glycosidic bond

Six beakers were obtained and each labeled separately as yeast, glucose, sucrose, maltose, fructose, and pyruvate. First, the 5.4% w/v solution of Saccharomyces cerevisiae yeast was created by measuring 5.4 grams of the yeast (obtained from Fleischmann's RapidRise highly active yeast) and adding it to 100 mL of distilled water in the beaker labeled yeast. Next, the 20% w/v solutions of each substrate were created. For glucose, 10 grams of glucose (obtained from VWR International /BDH, item # BDH0230)

concentration of glucose in both coke and Gatorade (Laboratory Manuel 2017) which will be done using a spectrophotometer. This is a method measuring both how much of a chemical substance is absorbed and the intensity of the light passing through the sample solution. (Vo, K. (2015). This also measures the concentration of NADPH which is a co-enzyme assisting the reaction which followed by spectrophotometry rely on different absorbance forms either oxidised or reduced, NAD+/NADH/NADP+/NADPH. NADPH responds well

laboratory technique was implemented to have the experience in the lab. Furthermore, the unknown solution needed to be investigated, to determine the solution’s macromolecules. Three methods were comprised to determine the unknown, but a set of other solution was used to help as indicators to match the unknown solution’s results. It’s consisted of biuret, iodine and benedict solution. Iodine solution is used to determine if the source had starch or glycogen. If the source had glycogen consisted

form cupric ion complexed with citrate in alkaline solution. When reducing sugars are presented with Benedict’s reaction, they will reduce the cupric ions (Cu²⁺) into cuprous oxide (Cu+), which is reddish orange. By testing solutions with Benedict’s reagent, we will be able to determine the reducing sugars based on their ability to produce cuprous oxide with a reddish orange product. It is expected that the positive control; reducing-sugar solution, and glucose will reduce the reagent and turn reddish

Background Research Water’s chemical composition is H20. It is two atoms of Hydrogen and one atom of oxygen combined. Water is a necessity. The human body can not go more than three days without a substantial intake of water. Ninety percent of the human body is water. All of our cells need water to do their daily objectives, and if we get to the point where there is not enough water the cell will not be able to do its intended function. The boiling point and the melting point of water is crucial

were being tested on throughout the experiment included: 1% glucose solution, 0.3% glucose-1-phosphate, 1% maltose sugar, honey solution, 1% sucrose solution, 1% lactose solution, 1% glycogen solution, 1% starch solution, protein, beer, distilled water and an unknown substance “unknown 193”. The Benedict’s test was intended to check if the twelve chemicals contained any reducing sugars. To test the substances, Benedict’s solution was added to each chemical and then the test tubes were boiled for five

So we understand the purpose of the unknown, positive control and negative control and explore various tests to find the presence of different macromolecules in a given solution. Carbohydrates are compounds of carbons, hydrogen and oxygen. They are usually found in ring structures and form polymers. Benedict’s test is a test for reducing sugars which are sugars with a free aldehyde (-CHO) or ketone (C-O) groups. This allows