Thermus aquaticus

The bacterium Thermus aquaticus is a fascinating organism that is utilized for a revolutionary process called a polymerase chain reaction (PCR.) The bacterium was discovered in the hot springs of Yellowstone, and is generally found in very hot climates, typically not livable by other bacterium. The bacteria belongs to the Archaebacteria kingdom, is a chemoautotroph, and is typically cylindrically shaped, but can be rod shaped or spherically shaped. PCR is a tool used by those looking to amplify

polymerase found in our bodies because when the DNA fragments of interest are heated in order to denature them and separate the two complementary strands, DNA polymerase also denatures. Taq DNA polymerase is taken from a strain of bacteria (Thermus aquaticus) that is taken from the hot springs of Yellowstone National Park, and it can withstand boiling temperatures which makes it effective for PCR. dNTP mix is a mixture of nucletotides that the polymerase uses to create the new

enough of the DNA amount for forensic scientist would help them match crime scene DNA to the actual suspect. PCR requires DNA polymerase. The specific name for the DNA polymerase that is being used in PCR is Taq polymerase. Taq polymerase is Thermus Aquaticus. The only way Taq polymerase can be used is if it is given a primer. In PCR, the researcher or student is required to choose the primer for the region of DNA that will be replicated. PCR primers are short, they are only about 20 nucleotides long

creates the correct pH and salt condition for DNA polymerase and because it also harbors magnesium ions in it, which serve as a cofactor for DNA polymerase. Taq DNA polymerase is vital because it is a heat stable enzyme derived from the bacteria Thermus Aquaticus that is able to withstand the high temperatures

Yellowstone National Park is a truly unique place. Few places in the world contain such amazing biodiversity, and such a wide variety of species in such a condensed area. While many scientists have been interested in studying the various plants and animals that inhabit Yellowstone, a group of microorganisms that has been of special interest in the past few years are Yellowstone’s extremophiles—the microorganisms that inhabit Yellowstone’s thermal springs. Extremophiles have opened the eyes of science

Aseptic Technique & Culturing Microbes Questions A. What is the difference between a bactericidal and bacteriostatic agent? What is the difference between sterilization and disinfection? Control of growth refers to the prevention of growth of microorganisms. This control is affected in two basic ways: by killing microorganisms or by inhibiting the growth of microorganisms

Extremophiles: adaptations and applications to physical extremes On the earth, millions of organisms live in various environments. They need certain temperature, pH, nutrient growth, certain range of salt, pressure, water and so on. However, some organisms can live beyond these conditions which mean they can survive in extreme environmental conditions called Extremophiles (Gupta, Khare et al., 2014). These extreme environments are: highly pressure, acidic or alkaline region, high temperature or

It is used to amplify a sample of DNA, creating thousands or even millions of copies of a certain sequence of DNA. "The story of modern PCR begins in 1976 with the isolation of Taq polymerase from the thermophilic bacterium Thermus aquaticus" (https://www.thermofisher.com/uk/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/spotlight-articles/history-pcr.html). However, it really became prominent in 1988 when

DNA is like the blueprint for the creation and proper functioning of every living organism. Organisms can sometimes be divided into prokaryotes and eukaryotes. Examples of prokaryotes and eukaryotes include bacteria and humans, respectively. These organisms must possess a method of replicating DNA, so a copy is provided for each cell that divides. Each cell’s responsibility is coordinated by the piece of DNA and thus, makes it a very valuable part of the cell and organism. So what are the methods

DNA Technology Task 3 – Poster DNA fingerprinting, also known as DNA profiling, is a technique used in forensic science that identifies individuals based on various characteristics of their DNA. Although the DNA sequences between humans are 99.9% identical, DNA fingerprinting is able to distinguish between individuals due to the presence of specific sequences within the non-coding region of the genome known as satellite DNA. This satellite DNA consists of long stretches of DNA made up of repeating