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3.6.03 Chemistry Lab

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3.6.1. BAP (Benzyl amino purine) stock solution (2mg/ml): 20mg of BAP being weighed and dissolved completely in 1N NaOH to a final total volume of 10 ml with autoclaved double distilled water to obtain a stock concentration of 2mg/ml was prepared and stored in clean autoclaved vials at -4°C.
3.6.2. IAA (Indole 3 acetic acid) stock solution (10mM): 1.0mg of IAA being weighed and dissolved completely in acetone to a final total volume of 10 ml with autoclaved double distilled water to obtain a stock concentration of 10mM was prepared and stored in clean autoclaved vials at -4°C.
3.6.3. 2, 4 – D (2, 4–Dichloro phenoxy acetic acid) stock solution (1mg/ml): 10.0mg of 2.4-D being weighed and dissolved completely in 1N NaOH to a final total volume

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