TYE ampicillin glucose agar plates: 15g Agar, 8g NaCl, 10g bacto-tryptone and 5g yeast extract should be dissolved in 800ml water (deionised). This solution should be autoclaved before cooling to 50˚C, and adding 1ml of ampicillin solution as well as 200 ml glucose solution before pouring plates. These can then be stored for up to a month at 4˚C, ensuring to dry in a flow-bench before use. M9 minimal medium glucose plates: 15g Agar should be dissolved in 800ml water (deionised) before being autoclaved. This should be cooled to 50˚C, before adding 200ml 5xM9 salts as well as 1ml of 1M MgSO4, 100micrograms of 1M CaCl2 and 1ml of 1mg/ml VitB1. 2xTY medium: 16g bacto-tryptone should be dissolved, as well as 10g yeast extract and 5g NaCl all within 1L of water (deionised), before being autoclaved and cooled to ambient temperature. Antibiotic and glucose solution should be added. Kanamycin solution: 50mg/ml Kanamycin powder should be dissolved in water (deionised) before being filtered through a 0.2micrometer filter and aliquots of 1ml should be produced. Ampicillin solution: 100mg/ml Ampicillin should be dissolved in water (deionised) before filtering through a 0.2micrometer filter, aliquots of 1ml should be produced. NHS-biotin solution: 10mg N-hydroxysuccinimido-biotin (NHS-biotin) in 1ml of dimethylsulfoxide (DMSO). Glycine solution: Take 100mM glycine at pH 7.4, dissolving 7.5g of glycine in 1 litre of de-ionised water before adjusting pH to 7.4 and filtering through a
Procedure: I used a ruler, thermometer, and scale to take measurements. I used a graduated cylinder, short step pipet, scale, and ruler to determine volume and density. I used a volumetric flask, graduated pipet, pipet bulb, scale, and glass beaker to determine concentrations and densities of various dilutions.
Step 4: Place the test tubes into separate coffee cups to maintain the upward position. Add 2 mls of the catalase solution to each of the test tubes and then place tubes1, 2, and 3 in the conditions described above. For test tube 4, fill the coffee mug half full of boiling
4) One package of active dry yeast was added to bottle labeled ‘5mL’ and solution was swirled.
Hydrate the yeast packets in a beaker with 400 mL of distilled water at a 10% concentration. In a 50 mL
9. If your biochemical test requires that a reagent is added, which tool do you need to use? (1 pt)
Add to this 5 drops of pH 4 buffer solution * Measure out 2 cm³ starch solution * Start stopclock and leave for 1 minute * Measure out 1 cm³ amylase and place in second corvette * Add to this 2 cm³ distilled water *
• Dilute some of the enzyme stock with buffer A to make a 4 mg/ml solution.
Preoperatively, a client is to receive 75 mg of meperidine (Demerol) IM. The Demerol solution contains 50 mg/ml. How much solution should the nurse administer?
The correct syringe is used to place 10 cm3 of the first glucose solution into the boiling tube.
The intravenous dosage in this case is 0.5 - 1 mg/kg IV (range: 0.5 - 2 mg/kg).
Pour 10 ml of the affinity gel into the column. Allow a few minutes for the solution to harden and transform into a gel-like consistency.
3. Use a sterile pipette to transfer 0.1 ml of each dilution on to a MacConkey agar plate.
After the hour has passed, remove the tubes and add 2-3 drops of biuret reagent to each tube and shake.
The beaker was then filled partially with distilled water; 1 ml of potassium iodide was then added, and the solution was tested for the presence of glucose. This data was recorded in table 1 on the data sheet along with the starting color of both the potassium iodide solution and the glucose/starch solution. The dialysis tubing was then submersed into the beaker containing the potassium iodide solution, and set aside for 30 minutes to allow maximum diffusion.
Preoperatively, a client is to receive 75 mg of meperidine (Demerol) IM. The Demerol solution contains 50 mg/ml. How much solution should the nurse administer?