The Effect Of Tye Ampicillin Glucose Agar Plates

1913 Words Mar 31st, 2015 8 Pages
TYE ampicillin glucose agar plates: 15g Agar, 8g NaCl, 10g bacto-tryptone and 5g yeast extract should be dissolved in 800ml water (deionised). This solution should be autoclaved before cooling to 50˚C, and adding 1ml of ampicillin solution as well as 200 ml glucose solution before pouring plates. These can then be stored for up to a month at 4˚C, ensuring to dry in a flow-bench before use.
M9 minimal medium glucose plates: 15g Agar should be dissolved in 800ml water (deionised) before being autoclaved. This should be cooled to 50˚C, before adding 200ml 5xM9 salts as well as 1ml of 1M MgSO4, 100micrograms of 1M CaCl2 and 1ml of 1mg/ml VitB1.
2xTY medium: 16g bacto-tryptone should be dissolved, as well as 10g yeast extract and 5g NaCl all within 1L of water (deionised), before being autoclaved and cooled to ambient temperature. Antibiotic and glucose solution should be added.
Kanamycin solution: 50mg/ml Kanamycin powder should be dissolved in water (deionised) before being filtered through a 0.2micrometer filter and aliquots of 1ml should be produced.
Ampicillin solution: 100mg/ml Ampicillin should be dissolved in water (deionised) before filtering through a 0.2micrometer filter, aliquots of 1ml should be produced.
NHS-biotin solution: 10mg N-hydroxysuccinimido-biotin (NHS-biotin) in 1ml of dimethylsulfoxide (DMSO).
Glycine solution: Take 100mM glycine at pH 7.4, dissolving 7.5g of glycine in 1 litre of de-ionised water before adjusting pH to 7.4 and filtering through a…
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