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Chapter 2
Background
2.1 Introduction
Chapter 2 will describe the essential background information needed to understand this
thesis project. A description of the biology of bone will be provided first and its focus
will be limited to femurs and tibiae, since those are the structures we have used
throughout this project. As well, we will briefly describe the analysis techniques that are
currently used to evaluate bones. Furthermore the theory behind the used technique and
instrumentation will be addressed. Previous bone studies using transcutaneous in vivo
Raman spectroscopy will also be described, as well as the differences between them and
the present thesis project.
2.2 Bone Structure and Composition
Bones
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12 Both the cortical and
trabecular structures provide ions like calcium phosphate, phosphorous, sodium and
magnesium that are basic to maintain the homeostasis of the bone.
Figure 1. Hierarchical structure of bone. Cotical bone is made of osteons (10-500 µm). Osteons are composed of concentric lamella (3-7 µm) and a Haversian cannal. A lamella is composed of mineralized collagen fibers, which are made up of collagen fibrils (0.5 µm each). A fibril is made up of collagen molecules and apatite nano-crystals.11
The three major components of bone tissues are: type I collagen, water and dahllite
crystals. 13 Dahllites are plate-shaped carbonated apatite crystals, which have “small but
significant amounts of impurities such as HPO4, Na, Mg, citrate, carbonate, K”. 14,15 The
apatite crystal lattice is then responsible for the degree of mineralization in bone tissue.
Type I collagen is the primary matrix component and its molecules are secreted by
Cortical bone
Osteons
Haversian canal
Concentric lamella
Mineralized collagen fibrils
Collagen molecule
Apatite nanocrystals 6 osteoblasts, these molecules will then arrange into collagen fibrils and subsequently to
collagen fibers, which are the basis to lamellae.
Type I collagen despite being the primary protein is not the only one present in
bone tissue, which also contains non-collagenous proteins.
It is made up of either liquid, solid, or connective tissue, within the matrix there are many types of connecting fibres, such as collagen and elastic fibres.
The skeletal system is made up of cartilage and bone. Both bone and cartilage are connective tissues, that is, they are composed of cells in a matrix with intracellular fibers. Just imagine connective tissue as a gelatin salad with grapes and coconut. The grapes would represent cells, the gelatin the support material for matrix, and the pieces of coconut the intracellular fibers. By changing the amounts of each ingredient and adding extra substances, we can produce a material that is very hard like bone and can withstand weight or softer like cartilage which can be used as a cushioning material.
Based on color, the cartilage was whitish in color while the bone was cream colored. The cartilage was relatively more flexible than the bone. On composition, bone is made up of calcium while cartilage is made up of hyaline materials. Upon piercing with a knife, the bone is resistant to piercing. On application of more force, it cracks and breaks unevenly as compared to the cartilage that is less resistant to piercing and was cut clean through upon application of a relatively lesser
Materials for this laboratory included Microsoft excel to compile data, a pen and paper to record data, a 15cm ruler, string, a caliper, and a two meter ruler. Since bones often times vary in width in certain portions of the same bone due to the presence of joints and processes the bone was measured at the midpoint along its length for its true width. Measurements
9. Hyaline cartilage is found on many joint surfaces, it has a very firm consistency. It contains no nerves or blood vessels, and its structure is relatively simple. Elastic cartilage, also called yellow cartilage, is present in the ears; it contains elastic fiber networks and collagen fibers. Fibrocartilage is found in the pubic symphysis, the annulus firbrosus of the intervertebral discs, menisci and the TMJ. During labor, the pubic symphysis is loosened to aid in delivery.
In this content am going to be talking and explaining the structure and function of the skeletal system. I will be talking about: Axial skeleton, Appendicular. Also highlighting the different types of bone such as long bones, short bones flat bones, irregular bones and sesamoid bones. I am also going to be talking about how your body is
A long bone is composed of 6 main parts. The diaphysis, epiphyses, metaphyses, articular cartliage, the periosteum and medullary cavity. (Tortora and Derrickson, 2011).
The slide below shows a section of the trachea (windpipe). Rings of hyaline cartilage embedded within the walls of the trachea provide support and help to maintain an open airway. Hyaline cartilage is the most common form of cartilage in the body, making up part of the nose, connecting ribs to the sternum, and covering the articulating surfaces of bones. When sectioned and stained, the matrix of hyaline cartilage takes on a light purple color. Cartilage-forming cells called chondroblasts produce this matrix, which consists of an amorphous ground substance heavily invested with collagen fibers. Chondrocytes (mature cartilage cells) can be seen singly or in groups within spaces (called lacunae) in
Bone is a living tissue and made up of cartilage. Fibrodysplasia ossificans progressiva bone appears as normal bone tissue, but it develops in the wrong places. Osteogenesis and ossification are medical terms which refer to the formation of bone. Most bones in the human body grow and heal up after a break through endochondral bone formation, which is how FOP bones grow. Cartilage forms first and then the bone will eventually take the place of cartilage.
Generally, bone can be classified into two categories according to its structure, cortical (compact) and trabecular (cancellous or spongy) bone. Cortical bone is stiff, with 5–10% porosity, and it makes up approximately 80% of skeletal bone, including cuboidal bones, flat bones, and the ends of long bones. Cortical bone is much denser, stiffer, and stronger than trabecular bone. The average strength of a compact human bone was 105 MPa in a longitudinal compression test, and was 131 MPa in a transversal compression test. In the same experiment, the average longitudinal strength in tension was 53 MPa [39]. In contrast, the porosity of trabecular bone is approximately 50–95%, which is higher than that of cortical bones. The surface area of the
The bone is hard, made up of collagen together with inorganic substances like calcium, magnesium and phosphorus.
Collagen is a fibrous protein which functions in the extracellular matrix and connective tissue. This protein strengthens and supports tissues in the body, such as bone, cartilage, skin, and tendons. Collagen 1 (alpha 1) is the most abundant type of collagen and is vastly distributed in connective tissue, except in hyaline cartilage. Type I collage is found throughout the body in the skin, tendon, vascular, organs, and it is responsible for strength and sturdiness. It is a triple helix formed by two copies of the alpha chain and one copy of the alpha 2 chain. Type II collagen is the main element of cartilage and it is formed by two or more collagens. Type III collagen makes up elastic tissues (skin, lungs, blood vessels). Type IV forms the
The skeletal system is made up of bones and joints. Bones are a dry dense tissue that is composed of calcium phosphorous and organic matter. The bones are protected and covered by a layer of fibrous connective tissue membrane called the periosteum (Brown, et al., 2015, p. 1547). There are two basic types of bone tissue: Compact Bone and Spongy Bone. Compact bones are dense smooth bones, while Spongy bones are composed of small needle-like pieces of bones and open space. Bones are then categorised according to the shape of the bone into four groups: long, short, flat and irregular. Long bones characteristically are typically longer then they are wide and generally have a shaft with heads at either ends e.g. the humerus. They are mainly compact bones. Short bones
Osseous tissue contains specialized cells, cell products, and a fluid matrix. The distinctive solid, stony nature of bone results from the deposition of calcium salts within the matrix. Crystals of calcium phosphate account for almost two-thirds of the weight of the bone. The majority of