A Research Study Of Microbiology

After many performed test such as the API 20E test strip, motility test, inoculated plates, gram stain, an identification flow chart which began with the results from an indole, MR, VP, and citrate test. This amongst many other test. These results brought the conclusion of my unknown culture to be Escherichia coli, my unknown number was 11.

You can grow microorganisms in liquid media or on solid media. Most bacteria can be grown in labs as long as the media contain a source of the major nutrients; carbon, nitrogen, sulphur, and phosphorous. They may also need to have other nutrients. These nutrients are made into a broth and the pH and salinity can be adjusted. The nutrient broth is placed in test tubes, which are plugged with cotton wool, capped with foil and then sterilised in an autoclave, at 121c for 20minutes. These tubes are then cooled before they are inoculated. To prepare the streak plates we dipped an inoculating loop into ethanol then placed it in the flame until the loop glowed red. Still holding the inoculating loop by its handle we removed the lid from

Entering human body is not easy. The bacteria and other harmful materials can enter human body either via food or cuts and injuries on the skin. However, there foreign agents are not always welcomed in the human body. There are immune cells that fight harmful agents. The immunity system in the human body identifies harmful microbes like bacteria, viruses, and others and provides defense to the body against these substances. There are antigens present in viruses, fungi, or bacteria and these antigens are normally proteins or toxins, chemicals, drugs, etc. that show the presence of foreign harmful agent. The immunity system of man identifies these antigens and fights the microbes producing them.

This research paper is a primary research paper because the paper indicates that a study was done by several biologist and scientist on the microbial community in the Rhizosphere. Therefore, all the research, answers, and conclusion they all concluded based on their study was explained throughout this paper based on all the information they gathered. Also, the authors explain the process and methods they used to carry out and conduct this research on the microbial communities. I came down to this conclusion by understanding what they wrote is backed up by evidence by explaining the procedure they carried out to study these microbial communities.

We selected two locations for sample collection: for Site 1 a phone was selected, and Site 2 a desk. One agar plate was labeled “Control” with our group identification number. A sterilized swab was dipped into a test tube with sterile water to wet the cotton on the swab. The culture from the swab was transferred to the surface of an uncultured nutrient agar plate using a zigzag motion. This is to determine if there are any bacteria in

After each technique was used, there were only results for the streak plate and the spread plate. No results were found for the pour plate. The bacteria can be counted for the streak plate, but not for the spread plate. It was found that using the streak plate technique, it would be easier to count the individual colonies. It was also discovered that doing the serial dilution to make sure that the agar is cooled before adding the bacteria.

The specimen was processed similarly to case-1. Escherichia coli was grown in aerobic culture, and Bifidobacterium sp. was cultured in anaerobic culture. The identification of Bifidobacterium sp. was done by both MALDI-TOF Vitek MS and Vitek-2. Escherichia coli was found to be Extended spectrum beta-lactamase (ESBL) positive and sensitive to Piperacillin+Tazobactam, Cefoperazone+Sulbactam, Imipenem, Meropenem, Amikacin, Gentamicin, Tobramycin, Chloramphenicol, and Cotrimoxazole. Bifidobacterium sp. was found to be sensitive to Penicillin, Ceftriaxone, Imipenem, Meropenem, Amoxycillin+clavulanic acid, Piperacillin+Tazobactam and Clindamycin and resistant to Metronidazole. The patient showed a good response to Meropenem and recovered completely.

This technique is used to help prevent the spread of infections and pathogens. Some examples of when health care professionals use this are when handling surgery equipment, vaginal deliveries, performing dialysis, inserting a chest tube and inserting central Iv. When working in the pharmacy you always want to make sure you have clean hands. They need to be cleaned with warm water and soap for at least 15 seconds. Another example would be is to make sure when your sneezing and coughing you always cover your mouth. Also if you're sick try to stay away from others or just stay home.

“ I feel that the greatest reward for doing is the opportunity to do more.” This quote was expressed by Jonas Salk, a microbiologist who created a vaccine for the Polio virus. My goal for the future is to one day enter the scientific field of microbiology. Throughout my educational career, science has always been my favorite course of study and I wanted it to be a supporting foundation behind the career field that I chose to enter.

Gone are the days when the scientists used the Liquid media for the study of microorganisms and used to grow them in the laboratories. Above all, when they had to segregate the microorganisms to study and gather information on the individual growth pattern and characteristics, the liquid media was not useful at all. In 1881, Robert Koch used aseptically cut slices of the potato as a solid culture medium which was supplemented with gelatin which was poured and allowed to solidify. This Technique however helped them to identify the discrete colonies of the microorganisms. Gelatin did not solidify during the summer and therefore Dr. Hesse came up with the concept of using agar-agar as the medium and this revolutionised the Microbiological Sciences.

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

Results and Discussion Our study was included 40 children patients and 10 healthy controls, their age was ranged from 3 to 12 years old, there were 25(62.5%) males and 15 (37.5%) females, as regard immunophenotyping 50% of patients were pre-B-ALL, 2.5% were mature B-ALL, early Int.J.Curr. Microbiol. App. Sci (2017) 6(9): 937-944 940 pre B 2.5%, Common B 35% (total: 90%) and 10% were T-ALL, as regard results of measuring BSP-1 levels by ELISA in patient group was 112.69 ± 11.54 ng/mL which was significantly higher than that of control group 8.58 ± 2.19 ng/mL (P value was 0.001) as shown in table 1, as regard CD44 variant 6 level by flowcytometry in patient group was 32.18 ± 15.26 which was significantly higher than that of control group 3.54

To date, the majority of microbiological study of soils have centered on bacterial and fungal presence in the context of plant mutualism [Figure 2]. Additionally, analyses of microbial biomass have typically utilized indirect models, such as change in soil carbon levels, as a measurement of microbial growth dynamics (Holden and Treseder 2014). When emphasizing ecological roles of microbes as nutrient cyclers, this is a useful methodology. However, it has led to a void in understanding of viral significance in these systems as well as a lack of detailed species-level identifications of bacterial and viral communities prior to and after fire events. The few abundance assays completed regarding bacteria in soil indicate that one gram of soil contains 108 to 109 bacteria and 109 to 1010 viruses (Williamson 2005, Van Der Heijden et al. 2008). This incredibly high number necessitates additional research, if only due to the fact that these organisms numerically overwhelm the system (Van Der Heijden et al. 2008). It would be irresponsible to ignore such a significant portion of the environment and attempt to come to ideal land management practices. It has become clear from even the broad scale respiration and nutrient assays completed that bacterial and viral communities are not a monolith; in different locations, phylogenetic community composition varies strongly (Williamson 2005, Goberna et al. 2015, Wang et al. 2015, Tas et al. 2014, Dooley and

Lauria-Bartani (LB) medium Davis et al., (1980). It was used for bacterial growth. It consists of (gm/l): Trypton; 10.0 gm, Yeast extract; 5.0 gm and NaCl; 5.0 gm. For solid medium, Agar Agar; 20.0 gm was added.