Microbiology: Unknown Discussion

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.

Citrobacter Freundii is a species of bacteria that can be potentially harmful to humans. It is known to cause meningitis by protruding into the brain and replicating itself (1). The Citrobacter species has also been found as a cause of some urinary tract infections, diarrhea, and even gastrointestinal diseases and symptoms (3). C. Freundii can be located in a wide variety of soils and water (3). Lastly, it is also the cause of many nosocomial infections due to its presence in water (1).

In class, we were given the task of identifying an unknown bacterium broth culture. After receiving number 69, I went through several tests to figure out what bacterium I received. First, I created a slide from my broth by putting a small amount of the unknown broth on to a clean slide and letting it dry for ten minutes. After this, I stained the slide by applying four reagents in order; crystal violet, grams iodine, decolorizer and safranin. From the stained slide, I discovered that this bacterium was gram-negative, which would determine the next couple of tests I would do to identify my unknown bacterium. I began by streaking for confluent growth from my broth culture onto a TSA plate. From the TSA plate, I aseptically transferred a loop

Catheterization is a frequently part of care for various surgical procedures. The study confirmed that patients lacked knowledge in catheterization and most of the patients were limited in decision making on when the catheter was to be removed. The clinical problem and research problem that led to the study being carried in the clinic was that the use of urinary catheter on patients, whom had not vacated the hospital lead to progressive bacterial settlement of the bladder at speed of approximately 5% per day. The increasing threat of the CAUTI infection, in the National Health Service steered to the

The definition of Microbiology is the branch of science that deals with microorganisms. Microorganisms are very small organisms that require the use of a micro scope to visualize them, which are bacterium, viruses, fungi (the most common) and others which are not so common such as parasites. Microorganisms are an essential element to the field of microbiology because it allows researchers in the field to find specific illness caused by a specific organism, cures for illnesses, medicine, history of an organism, and the epidemiology of an organism.

One of the most common bacteria known to man is affiliated with the genus Staphylococcus. Staphylococcus aureus can be described as bacteria that present as gram positive cocci either in pairs, tetrads, short chains or irregular grape like clusters (2A). With their diameter ranging from 0.5 to 1.5 um, other characteristics that comprise this bacteria include their non-spore forming ability and nonmotile ways (2A). Defined as facultative anaerobes, these microorganisms are usually encapsulated(2A) and are a member of the normal flora that resides on humans(2D). In humans, a common site of habitation would be the nares(2D). The chances of S.aureus colonization are greater in individuals that are diagnosed with hiv, insulin-dependant

Escherichia coli is a normally harmless gram negative bacteria, commonly found in the digestive system of humans and animals alike. E. coli is a vital part of the digestive tract, but outside the digestive

Aerobes are organisms that grow only in the presence of oxygen. They obtain energy from aerobic respiration.

In order to identify the unknown #75, there are several key concepts that should be remembered to successfully identify the bacteria. Initially a broth of an unknown was given containing both positive and negative unknown bacteria. It is important to observe aseptic technique to successfully and accurately identify the unknown bacteria that was given.

For this analysis assignment, I used the American Type Culture Collection (ATCC) website to find technical documents from the microbiology field. I chose two whitepapers written by the same author but the papers themselves have vastly different purposes. The first white paper informs the reader of multi-resistant strains and the need for new solutions to this problem. The second document discuss a new approach to treating multidrug-resistant infections. This memo will explain the purpose of each document by comparing the intended audience, genre, conventions and the appeals used. AUDIENCEAs stated above, both documents discuss multidrug-resistant organisms. Despite their similar topic, each has a different primary audience. The first

Me: Question #1- How can you briefly describe what a microbiologist is to those who do not know?

Escherichia coli (E. coli) are Gram negative bacteria. They ordinarily live in the insides of individuals and animals. Generally E. coli are safe and really are imperative ordinary vegetation of a sound human intestinal tract. Nonetheless, some are pathogenic and cause

Microorganisms have lived between us on the earth for more than 3.8 billion years and show the greatest metabolic and genetic varieties. They are vey essential component and play an important role in maintenance of the ecosystem. They compose almost 50% of the living biomass. In order to survive, microorganisms have developed different mechanisms that enable them to respond to selective pressure applied by environment and different challenge. For example; microorganisms that cause diseases for human have developed resistance mechanism to fight antimicrobial agents. (Byarugaba.D.K.,2009)

Escherichia coli, also known as E.coli is a bacterium that lives in the intestine of warm blooded organisms that causes sickness and several diseases particularly in the weak

The Skaneateles Conservation Area, including the primary forest and the secondary forest, located in Onondaga County, New York. Primary forests and secondary forests are two major groupings of land in Skaneateles forests. A primary forest is a land that is untouched by man and the ecological processes of it were not disturbed, while the secondary forest is a land that was agriculturally used and the ecological processes of it were disturbed. Microbiology is the study of small life. The soil in both the primary forest and the secondary forest is one of the main receptacles of microbial life. The most abundant microbes in soil are bacteria. According to Gregory McGee in microbiology, a pure culture is very important as it is derived from a colony (McGee, 2016). In this experiment, we used the streak plate and the sterile techniques methods. The sterile technique was used to avoid contamination of sterile media and equipment during cell culture of unwanted microbes. Sterile technique is one of the best techniques to minimize potential contamination. In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism. It is essential to avoid contamination as a heavy contamination of the soil could result in reducing fungal biomass and alter the composition of the fungal community (Mülle, Westergaard, Sørensen, and Christensen, 2001). While it is important to understand how contamination could affect the procedure, it is