A summary analysis of the article “Microarray detection of food-borne pathogens using specific probes prepared by comparative genomics.”

1531 Words 7 Pages
In the field of public health, food-borne illnesses are a major concern because it has been estimated that each year 76 million cases occur in the United States causing 5,000 deaths (Suo et al., 2010). In 2008, the Center for Disease Control and Prevention’s FoodNet surveillance program reported over 18 thousand cases of food-borne illnesses occurred in 10 states (Center for Disease Control and Prevention [CDC], 2008). According to estimates from the CDC (2011), the most common food-borne pathogens that maybe seen in the United States are Norvovirus (58%), Clostridium perfringens (10%), Salmonella (11%), Campylobacter spp. (9%), and Staphylococcus aureus (3%). Among the other 9% (not published) include Escherichia coli
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The emergence of DNA based technologies such as the polymerase chain reaction (PCR) and microarray analysis have been utilized in the rapid detection and identification of many pathogenic bacteria (Mothershed & Whitney, 2006; Versalovic & Lupski, 2002). The expansion of these technologies has significantly enhanced the sensitivity, specificity and the rapid detection of microorganisms (Suo et al., 2010). Microarray technologies have the potential to perform high-throughput detection of multiple pathogens. Recent work with specific oligonucleotide probes suggests that pathogen detection can be performed on a sample that contains a mixed culture of bacteria (Kim et al., 2008). This paper will
Purpose of Research:
Previous work with DNA based technologies to accurately detect and identify human pathogens has been demonstrated. Furthermore, three methods have been utilized: 1) amplification of one or more universal genes (16S rRNA and 23S rRNA) through PCR, 2) amplification of pathogen-specific markers (toxins, virulence factors) using multi-plex PCR and 3) amplification of random DNA fragments (Kim et al., 2008). Kim et al. (2008) state that the first two methods is flawed due to the limited number of probes utilized and that the amplification of universal genes would not discriminate below species level due to the fact that these genes are highly conserved within the genus. On the other hand, Kim et al. (2008) reported that