Title: “Identification of Unknown #1” Purpose: Discovering and studying new bacteria can lead to new innovations and possible cures for existing or future diseases. The investigation of bacteria can also help incubate ourselves or act quickly in response to an invasive strain. The commercial food industry has a great need in identifying Salmonella to prevent disease in consumers. As well as being the cause of enteric (typhoid) fever, an important infectious disease, Salmonella is perhaps best known as a cause of bacterial food poisoning. Although typhoid fever has been largely eradicated in the developed world, Salmonella food poisoning has long been, and continues to be, an important global public health problem. In much of Europe …show more content…
I knew that the Methyl Red portion of the MR-VP confirmed that the pH was less than or equal to 4 making this quite an alkaline agent, and reconfirmed alkalinity with the final experiment with the use of the litmus milk broth turning blue. The Gram negative stain and rod shape eliminated six of the possibilities. The SIM (Sulfur Motility and Indole) testing proved that the organism was motile, was a gas producer, did not produce indole, and Triple Sugar Iron-Agar Slant produced a black butt and red slant indicating glucose fermentation and confirmed sulfur gas production, leaving only Proteus vulgaris as another possible outcome. Casein, gelatin, and urease testing were needed to conclude my results, as only P. vulgaris would be found to possess the enzymes casease, gelatinase, and urease, which, none of those were found in my unknown organism. Both of the two organisms were negative to hydrolyze starch, and neither can metabolize oxygen. Most infections with Salmonella are traced back to dairy, poultry and meat products, but Salmonella can grow on just about any food. Chickens and eggs are particular high risk foods. Proper storage with the use of cool temperatures in a refrigerator, proper handling including cooking
In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. A Gram stain using crystal violet, iodine, and safranin and a simple stain using methylene blue characterized the organism’s cell wall. Cultural behavior was classified by inoculating the organism onto nutrient agar and incubating it at 37° C for 48 hours, and observing its behavior, as well as using SIM medium to test for motility. Optimal growth temperature was
Salmonella is a bacterial disease that occurs in the intestines, the signs and symptoms can be; fever, or other illnesses such as diarrhea and abdominal cramps. People typically get salmonella from contaminated foods, which seems to occur frequently from poultry and eggs (Nordqvist, 2016). This is just a basic look at salmonella though, next we need to look at the epidemiologic triad (host, agent, and environment) and see how each plays a role in a salmonella outbreak.
This case study, finalized and updated onto the Centers for Disease Control and Prevention, talks about the Multistate Outbreak of Salmonella Wandsworth Infections Linked to Veggie Booty. Publishing this outbreak onto their website makes it very beneficial and helps the public realize the importance of food health. Food health is just as important as overall public health. It is essential because people need to consume food in order to live, and if their food is tainted or contaminated, it would cause health problems and sickness and in some extreme cases, death. Ensuring food health will overall promote public health in the long run.
This multistate outbreak of Salmonella Tennessee case was published by the Centers for Disease Control (CDC) in March of 2007 to inform the public of the outbreak that was discovered and in order to prevent other people from getting sick. The Salmonella infection was associated with the consumption of Peanut Butter, specifically with Peter Pan peanut butter and Great Value peanut butter brands manufactured by ConAgra. Since both brands were manufactured in the same facility in Georgia, they both became contaminated and so, the peanut butter jars with the code 2111 were to de discarded and avoided (MMWR, 2007). However, since the Great Value peanut butter brand is manufacture in other plants, only the peanut butter jars from the facility in Georgia had the bacterial infection within them. Although Salmonella is a common illness in the United States estimated at 1 million cases per year, the Tennessee serotype is a rare case and it was first associated with peanut butter in February 2007; however, since November of 2006, public health officials had noticed an increase with this serotype (MMWR, 2007). The symptoms of Salmonella Tennessee include diarrhea, fever, abdominal cramps and dysuria, which can be seen in 12-72 hours from the person being contaminated (MMWR, 2007). Furthermore, 715 Salmonella Tennessee cases where identified in 48 states from the beginning of August 2006 through the end of July 2007 (Sheth, 2011). The source of
The infectious agent (pathogen) that causes salmonella is called salmonella enteriditis. The bacteria is larger than a virus; but, is visible to the eye with the microscope. It is rod-shaped, gram negative, non-motile bacteria that does not form spores. Interestingly enough it communicates to its fellow bacteria via AHL. It infects the cell, multiplies within it; then, bursts the cell. Special effector protein factors are required for salmonella intestinal invasion and the induction of fluid secretion and for inflammatory responses. There are about six names species names of it, salmonella
Exposure to salmonella is not taken lightly, as it can prove deadly and lead to contact with several related diseases such as salmonellosis. Salmonellosis can manifest with symptoms including diarrhea, severe cramping, and
Gastrointestinal diseases are pervasive problem that is caused by ingestion of contaminated foods or water in which an extensive etiological agents known as enteric pathogen are present, [1]. Salmonella is one of the most opportunistic gram negative, facultative anaerobic food borne pathogens that represent a major health problem and a substantial cause of food poisoning[2]. Salmonellosis caused by Salmonella enterica serovar Typhimurium (S. Typhimurium) stance as the second most prevalent cause of acute gastroenteritis that affecting one million illnesses in the United States, with 19,000 hospitalizations and 380 deaths[3]. Infection begins with the ingestion of contaminated food or water then characterized by attachment of the bacteria by fimbriae or pili to cells lining the
Have people ever thought about the risks of on farm salmonella, and the danger it poses to hog herds as well as the public health? Salmonella control starts at the beginning of finishing farms, breeding herds, feed suppliers, and on to the slaughter houses. Salmonella control programs have already been established in several European countries to help reduce their salmonella on hog farms. Salmonella is one of the most important food borne pathogens as well as the second most common source of zoonotic human infection. Farmers and veterinarians are striving to pursue an effective plan to decrease salmonella on hog farms through several ways of prevention.
There have been numerous times where I have been affected by Salmonella, so it was easy to swing towards Salmonella as the CBRN choice. Salmonella is found in everyday food products such as raw chicken, raw beef, raw fish, raw eggs and unpasteurized milk (foodsafety.gov). Salmonella can also be found on reptiles, amphibians, birds and even pet treats (foodsafety.gov). People will start to feel symptoms within four to seven days which include: nausea, vomiting, abdominal pain, diarrhea, fever, headaches, dehydration and possibility blood in the stool (mayoclinic.gov).
It is important in the way food is handled and the safety of the process that food goes through. One of the most common causes of food poisoning is bacteria and viruses. Symptoms of food poisoning can range from very sever to not very, depending on what bacteria or virus is the cause. Salmonella is the name for a common group of bacteria. The bacteria can effect different people in different ways. It can cause a serious sickness in older adults, infants, and persons with chronic diseases. Cooking and pasteurization can kill Salmonella. Two caused by this bacterium are the lettuce recall from major supermarkets and the mayonnaise contamination on Melbourne Cup Day, by Piccalilli Catering.
Salmonella: “the name of a group of bacteria, is one of the most common causes of food poisoning in the United States” (U.S. Department of Health & Human Services 2015). Salmonella is usually found in contaminated eggs, poultry, meat, cheese, fruits, vegetables, etc. Symptoms may include diarrhea, vomiting, cramps, and fevers. Salmonella usually last between 4-7 days while the best course of action is to hydrate with clear fluids and get plenty of rest. The best way to avoid catching this bacteria is to have proper cleanliness, cook foods to direct temperature, refrigerate food, and keep it separated.
Salmonella is known as a gram-negative, rod shaped bacillus. It is a facultative anaerobe in the family Enterobacteriaceae (Todar). There are more than 1,000 known types of Salmonella, with ranging levels of toxicity (Jones), accounting for 60% of all bacterial diseases (Curtello). Salmonellosis has affected over 1.4 million people per year in the United States, including more than 500 fatal cases (Jones). This organism has a wide host range with abilities to attack both animals and humans (Tartakow). Salmonella’s main habitat is in the intestinal tracts of warm blooded animals (Jones). It best grows at a temperature between 5 and 47 degrees Celsius (Pui). Non-host adapted Salmonella may reside in food or water
Abstract: Salmonella is a bacterium that causes one of the most common intestinal infections in the United States - Salmonellosis. The chance of contracting this disease is significantly high, and more and more people are suffering from the symptoms and complications of Salmonella. This paper will discuss about the disease itself, the current outbreaks that are related to this disease, preventions and the treatments.
Studying Salmonella is vital in understanding how SafD operates and possible clinical problems posed to individuals, due to the different strains of the bacterium that have been previously discovered. Although Salmonella has been identified and classified into different classifications, it has undergone multiple changes and the main nomenclature system is yet to be established (Chiu, Lin-Hui, 2014). The genus which Salmonella belongs to is known as the Enterobacteriaceae family (Chu and Su, 2004; Farmer, 1995). Salmonella is a gram negative rod shaped
* Ensure public safety: A majority of salmonella species can be inactivated if the restaurant chain operates at documented pasteurization temperatures. However, in the event of the infection reaching the public, there is a significant morbidity and mortality rate. Hospitalization and death rates can be as high as 33% and 3% respectively. (Schlundt, 2001) Exhibit 1 shows the number of Salmonella-related deaths and death rates in the past 10 years.