Anemia Testing

Peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, H2O2, into O2 gas and water. We examined the different pH environments that can affect the enzyme activity during the breakdown of H2O2. In order to do this, we added different levels of pH, low, medium, and high, into different test tubes with the enzyme and H2O2, and we then inverted the tube. The amount of O2 gas produced was then measured and recorded. The result was that the higher pH produced more gas, followed by medium pH, then low pH. The enzymes were more active in the pH of about 10. It increased

One of the best-studied peroxidases is horseradish peroxidase (HRP), which has a heme-iron co-factor. In most heme-peroxidases the iron atom in the active center undergoes a reversible change of its oxidation state. The reaction proceeds in three distinct steps. In first step, the resting state high-spin Fe(III) is present, which is oxidized by hydrogen peroxide to form an unstable intermediate called compound I (Co-I) with Fe(IV), releasing water in the process. Compound I is not a classical enzyme–substrate complex, but rather a reactive intermediate with a higher formal oxidation state (­5 compared with ­3 for the resting enzyme). Thus, compound I is capable of oxidizing a range of reducing substrates. This reactive intermediate oxidizes

The research and observations of this lab primarily focused on the enzyme activity of the enzyme Peroxidase. Peroxidase is a large protein and is composed of more than three hundred amino acids. The enzyme was selected as it is easy to experiment with and effectively showcases the effects of varying independent variables, such as pH and temperature. Peroxidase catalyzes the decomposition reaction of the chemical Hydrogen Peroxide ( H2 O2 ) into water and an electron donating molecule, which stands for R in the written chemical equation. ( The equation is displayed below:

Abstract: This lab was developed to investigate blood glucose and diabetes. Diabetes is a lifelong chronic disease in which there are high levels of sugar in the blood (Diabetes). The spectrophotometer was applied to this lab to determine the absorbance of blood glucose in diabetic and non-diabetic blood samples. In order to prove this, tests were conducted by taking the blood samples at different times right before a meal was eaten then 30, 60, 90, and 120 minutes after the meal. The 6 test tubes had been placed in the spectrophotometer to measure the absorbance of blood glucose in the diabetic and non-diabetic blood. It was hypothesized that people with diabetes will absorb more

3.Blood Glucose: Monitor fasting blood glucose and random blood glucose to assess the glucose control and response to treatment. Ideal FBS is 4-6mmol/L.

As is the case for almost every mechanism in an animal body, oxygen is required for metabolic processes. This process, aerobic respiration, is the production of cellular energy in the presence of oxygen. Due to this fact, the metabolic rate of crayfish can be studied and calculated based on the rate of oxygen consumption for the organism in question (Meade et al, 2002).

The development of the breath acetone biosensor was performed within the school grounds. Vanillin and sodium nitroprusside were used as the color-forming molecules for the detection of acetone.

The concentrations of hydrogen peroxide being tested are: .1 ml, .2 ml and .4 ml. A similar process from part one will take place; therefore, procedures from part one can be used in part two just different quantities of substances. Every 20 seconds record absorbance for a total of 3 minutes using the spectrometer. These steps will repeat for every enzyme concentration’s control, substrate and enzyme test tubes. The total amount of data points should be 6, 2 data points per concentration.

The enzyme also can catalyze the reduction of cytochrome c by tetramethyl-p-phenylenediamine, which is a chromogenic reducing agent. This agent is a chemical that changes color as they become oxidized. I inoculated the unknown organism onto a petri dish the day of the Gram Stain test. The following day of the Oxidase test the organism had grown on the petri dish ready for testing. I added five drops of oxidase reagent over the growth and saw the results after 20 seconds.

Some aerobes have the enzyme called cytochrome oxidase, which is a molecule that is a terminal electron acceptor in the Electron Transport Chain (ETC). A Q-tip was used to pick up the unknown organism and then administrated a drop of reagent, Dimethyl-p-phenylene diaminic hydrochloride. Results were obtained within a minute duration. If purple was observed in less than a minute, it was positive for oxidase. If there was no color change within a minute, it was negative.

First, γ-cyclodextrin and pyrene can sensing of steroid and terpenes. γ-cyclodextrin will bearing a pyrenyl chromophore anchored at lower rim which formed the self-inclusion dimer in 10% dimethyl sulfoxide (DMSO) aqueous solution which can exhibited excimer emission at 470nm. Self-inclusion dimer

The process our prototype package covers is how to assess the state of a patient's essential body functions using vital signs. In order to check a patient's vital signs you must assess their temperature, pulse rate, respiration rate and blood pressure. This process is used by college students who are pursuing a career in the medical field. The part of this process we will be showing is how to properly check someone's pulse and respiration rate.

AThe major new capability from the previous members of the animus pump family,of the Animas vibe is the continuous glucose monitor CGM integration. A CGM is a wearable device that measures glucose levels in body fluids. It is’s able to give trends, and alert young and old users a like to take action when their sugar is low or high. Before the Vibe was introduced, users would carry two separate devices which each had separate suite of software to sync with a computer and share with their health care provider. This made diabetes management difficult, and added another device to the large arsenal of things that tools that one diabetics must depend on a daily basis. The integration of the CGM and insulin pump was revolutionary, yet because it was not approved by the FDA, users were left with only inconvenient, outdated equipment .

That is, the use of sweat, saliva and other body exudates, by calculating the concentration of glucose and exudate glucose glucose concentration in the measurement of blood glucose. Among them, the United States Medtronic first launch of the FDA approved blood glucose real-time continuous monitoring system (CGM). The system consists of a disposable continuous blood glucose detection probe, a radio frequency transmitter, and a receiving display. The probe can be attached to the patient's abdomen with a small wire (the wire is extremely small, the penetration is extremely fast and painless), and the glucose concentration in the subcutaneous interstitial fluid is measured every 10 seconds for 3 consecutive days. Through the wireless means to the receiver, the receiver every 5 minutes for the average data processing, and then converted to blood glucose stored value. This method of information collected every day refers to the blood test method of 100 times. In addition, developed by the United States Spectrx blood glucose tester is the use of laser cuticle in the skin to open a row of pores (and no pain), and then by a special sensor to collect interstitial fluid and measurement of blood glucose analysis, the same wireless technology has also been applied to monitoring blood pressure and oxygen level in the blood and so on, it has received large amount of investments

Investigating haemoglobin (Hb) concentration in blood samples using the haemoglobincyanide method and in foetal haemoglobin samples