Capillary Action Chromatography Essay

Background: Paper chromatography is one method for testing the purity of compounds and identifying substances. Paper chromatography is a useful technique because it is relatively quick and requires small quantities of material.

In this lab, a mixture of three solids was separated to their individual components through decantation,

Some of the classes are sometimes larger than others. The larger the class, the less discriminating the identification, but all such associations provide relevant information (Kaye, 2009). Identification is the process of determining a substance’s physical or chemical identity of a substance such as drug analysis, species determination and explosive residue analysis as near absolute certainty as existing analytical techniques will permit (Saferstein, 2014). These are forms of physical evidence that become crucial to solving a crime. With the use of new technology, the scientists are able to determine if a known sample of a small fibre, paint chip, or shoe pattern is consistent with the unknown sample found at the scene. When individual characteristics are determined, the suspect has been linked to the

For part one of this experiment, I only experienced separation of colors with the green and brown M&M’s, along with the yellow food coloring. The green M&M separated into yellow and blue, with blue travelling farther up the paper. It is not surprising that green separated into blue and yellow because those are the primary colors that make up green. The brown M&M separated into red and orange, with the orange travelling farther up the paper. Finally, the yellow food coloring separated into yellow and red, with yellow travelling farther up the paper. This could be because it was such a concentrated, small amount of food coloring. The colors that didn’t travel very far up the paper, such as orange and brown, are probably less soluble than the others, like blue and green.

Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct?

The stationary phase will absorb or slow down different components of the tested solution to different degrees creating layers as the components of the solution are separated. Chromatography was invented by the Russian botanist, Mikhail Tsvet. Chemists use this process to identify unknown substances by separating them into the different molecules that make them up.

Pigments extracted from different greens have different polarities and may be different colors. Mixed pigments can be separated using chromatography paper. Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a, chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility, which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance, followed by chlorophyll a. Chlorophyll b is the most polar; therefore, it travels the shortest distance. The separated pigments on the chromatography paper can be eluted in acetone and absorbance spectrum is

A chemical used in the film that is apart of forensic science is a gel like substance that is placed under the

The scientific examination of evidence in criminal cases found in a crime scene is called forensic science. Forensic scientists use the same technology, tools and methods used by other scientists doing other types of research, including microscopes, computers, and lasers. As forensic science has advanced over the years, so has the ability to gather evidence and solve crimes. At crime scenes, lasers provide lighting to track blood sample that not visible to the naked eye. Forensic imaging technology is technology that lets the first responding local police department or crime scene investigators immediately send a photo or fingerprint image to a central database for identification. Computers can enhance pictures taken by a camera and use features like the facial recognition software to clearly identify blurred images. Lasers can also vaporize small portions of paint specimens to determine the exact paint used on a car in a hit-and- run case(How Forensics Work). Although this science has been used for years, wrongful convictions have brought to the attention that forensic science has been faulty for decades. The chilling truth is that forensic science has contributed to convicting innocent people over the decades and will continue to do so if the normality continues.

Electrophoresis is a chromatography technique(separation of particles) through which molecules are separated according to their size in a fluid such as DNA, RNA, and proteins under influence of an electric field(Electrophoresis). The word comes from Greek and English language; phorēsis is a Greek word which means movement, whereas prefix electro means that electricity is being used. All DNA fragments are separated on the basis of the size since all are the same molecule (Gel electrophoresis).

Chromatography is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the original mixture depends on how strongly each component is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase will be retarded and not move alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.

It is possible to separate these pigments from each other using a technique called paper chromatography. In this process, plant tissue extract is applied to a piece of chromatography paper. “A solvent is allowed to travel up the paper, and if the pigment is soluble in the solvent, it will be carried along with it.” (Benya, 2009) Different pigments have different affinities for the solvents or polarity and will travel at different rates. Chlorophyll, anthyocyanins, and carotenoids are typically non-polar.

The next step was to place the strip of chromatography paper on a paper towel. Then dip a capillary tube into the plant pigment extract (spinach pigment extract) provided by the teacher. The tube will fill on its own. We applied the extract to the pencil line on the paper, blew the strip dry, and repeated it three to four times until the line on the paper is a dark

Chromatography Investigation Chromatography is a highly regarded technique used to separate the components of a mixture. It is based on the principle that each component possesses a unique affinity for a stationary phase and a mobile phase. The components that are more inclined to enter the mobile phase will migrate further on the chromatogram and distinguish themselves from the other components. The type of solvent used in chromatography is known to directly affect the separation of the mixture. In this experiment, thin-layer and column chromatography will be utilized to separate the numerous chlorophyll and carotenoid pigments of a spinach extract.

The reason for conducting the research is to know how the colors of our favorite candy-coated sugary rewards came to be and that method of knowing is what we call “Candy Crhomatography”. Chromatography by definition according to Webster’s dictionary means “ a method for separating the constituents of a solution (gas or liquid) by exploiting the different bonding properties of different molecules”. In