Carbon Dioxide Experiment in Photosynthesis

In the dishes, I dropped the appropriate treatment into the center, where the marks were made. Next, I closed the petri dishes, taped them up, and let them sit at room temperature for a week. Then I opened them up to take two measurements. The first measurement was the number of seeds germinated. The second measurement was to measure the seedling lengths.

1. Fill the graduated cylinder nearly to the top with water, with a tall glass tube open at both ends (the water level with act as the closed end).

27. Heat the water to 40°C with an alcohol lamp by setting up the apparatus as shown in figure 3 below:

The first step that needed to be done in this experiment was adding hydrochloric acid (HCl)

Add to this 5 drops of pH 4 buffer solution * Measure out 2 cm³ starch solution * Start stopclock and leave for 1 minute * Measure out 1 cm³ amylase and place in second corvette * Add to this 2 cm³ distilled water *

Add three seeds to the potting mix and cover seeds with little remaining potting mix. After the addition of the potting mix, use a dropper filled with water and water each cell until water drips from the wick. Then place the quads on a watering tray under the fluorescent light bank. Each cell should have an equal distance from the light bank. Quads should be three inches below the fluorescent light; the light should also be left on all day. Make sure all wicks are in contact with the mat that sits on the watering tray. Also watch out for the watering system regularly throughout the experiment. After four to five days record plants in the quads, giving their phenotypes in a table for each cell removed all but the strongest plant.

Fill a test tube about 1/3 full with cold tap water for use in step 34.

Abstract: The purpose of this lab is to separate and identify pigments and other molecules within plant cells by a process called chromatography. We will also be measuring the rate of photosynthesis in isolated chloroplasts. Beta carotene, the most abundant carotene in plants, is carried along near the solvent front because it is very soluble in the solvent being used and because it forms no hydrogen bonds with cellulose. Xanthophyll is found further from the solvent font because it is less soluble in the solvent and has been slowed down by hydrogen bonding to the cellulose. Chlorophylls contain oxygen and nitrogen and are bound more tightly to the paper than the other pigments.

9.Repeat the procedure with a new mass of baking soda. Before beginning, rinse the reaction vessel with water. Refill the graduated cylinder with water. Check water level in collection box so it has room for the water from the graduated cylinder.

Break an Alka-Seltzer tablet into a few small pieces and drop them into the container one at a time. When the bubbles stop add more pieces of Alka-Seltzer to see it again.

3.Measure and add 0.5g, 1.0g, and 1.5g of sucrose into 3 of the test tubes. Do not add sucrose into the 4th test tube because this will be the control. Lightly shake the test tube to mix the contents together.

The third step that was taken was germinating the seeds. Two sets of paper towels were used to germinate the

Remove the tubes and add 2-3 drops of Iodine – potassium – iodide solution to each tube.

     Photosynthesis is a reproductive system that occurs in plants. The main components required for photosynthesizing are sunlight, energy, water and mineral from soil, and carbon dioxide from the air. Once these components are combined they

3 drops of Lugol’s solution is added to each tube. Presence of starch is indicated by dark purple color occurrences. The amount of starch is indicated by the shades of reddish brown.