Danlos Syndrome

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Abstract In this study, we screen for the dumpy phenotype in Caenorhabditis elegans. These worms are shorter and stouter than the wildtype. By screening for the dumpy phenotype among mutagenized worms, we are seeking to find answers how the dumpy First, we address our hypothesis: there are specific genes within the C. elegans genome that code for the biological process of movement and therefore are responsible for the roller phenotype. After isolating mutant roller worms, we can then cross-breed them with wild type worms to test the nature in which the gene is inherited (i.e. dominant or variable penetrance). We can also map the gene associated with producing the roller phenotype, which could then help elucidate the gene homolog in …show more content…

( Darwin J Prockop)
In this experiment we will focus on Ehlers- Danlos Syndrome which is caused by a mutation in the gene COL3A1. Ehlers Danlos syndrome is common name given to more than ten different inherited disorders. All these have a genetic defect in the collagen and connective tissue. Ehler- Danlos syndrome affects the skin , joint and blood vessels. Ehlers- Danlos Syndrome IV is associated with arterial rupture and visceral perforation(Robert A Schwartz,). Currently there is no cure for Ehlers- Danlos Syndrome. What i’m proposing is that through a forward genetic screen you can identify the
The potential to apply forward genetic approaches for comprehensive genetic dissection of vertebrate development was the initial attraction for researchers to utilize the zebrafish as a model system. Following is an overview of the major forward genetic approaches employing the zebrafish, along with examples that have provided fundamental new insights into vertebrate …show more content…

We will mutaganize a stock A population of N2 C. elegans, the P0 generation, will be acquired and mutagenized by Ethyl Methanesulfate (EMS). Individual worms from this generation will produce spatially separated F1 offspring. A select few F1 individuals will reproduce further and produce an F2 progeny.
● The F2 generation of this test group will then be screened for the roller mutation. The progeny of these rollers, the F3 generation, will yield the most integral data of our screen.

. These foundational worms will serve as our parent generation, P0. Once these worms have reproduced and eggs are visible on the plates, the P0’s will be moved to another 5 plates. This will ensure that we have ample offspring of the F1 generation in case issues such as contamination or starvation occur. Also, to safeguard against starvation, we will transport only 5-7 F1 worms to new plates to lay eggs, which will hatch to become the F2 generation. In addition to providing the worms with an ample food source, moving each successive generation of worms to fresh plates will allow us to more efficiently keep track of the offspring and the mutations observed in each generation. This is why it is imperative to maintain a strict schedule during the course of the experiment. Checking on the worms daily, we can see exactly when eggs are laid and when parent worms need to be removed in order to prevent mixing of

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