Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method

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Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method Prepared for: By: Date:

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This study is to examine the effects of different types of disinfectants by disk diffusion method using common agents such as Betadine, Clorox, Crest, Kiss My Face, Listerine, and Lysol. Wound care and general cleaning of home surfaces is a part of daily activity for many of the general population. Knowing which agents are effective against the bacteria that most commonly cause infection, disease, and illness allows educated decisions on which agents to use. We compared the bactericidal effectiveness of 6 common agents widely used by the general
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Crest (Cr) – A widely used antimicrobial for oral bacteria and biofilms. Especially useful on Gram-negative micro-organisms such as Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens, and Fusobacterium nucleatum. Kiss My Face (Kf) – An organic alternative to typical alcohol based mouthwashes using aloe vera as antimicrobial agent and grapefruit extract as a polyphenol. Proven useful with Trichophyton mentagrophytes and Staphylococcus aureus._ _. Listerine (Li) – An alcohol based antiseptic mouth rinse containing the active ingredient ethanol which is toxic to bacteria at concentrations of only 7%. Reduces dental carries, plaque and gingivitis. Lysol (Ly) – A O-phenylphenol preparation of cresols used for surface cleaning in various environments such as homes and hospitals to kill microorganisms such as fungi, Tuberculosis, certain viruses,_ staphylococcus_ and Pseudomonas bacteria. Does not kill nonenveloped viruses, such as _parvovirus, polyomavirus, papillomavirus, adenovirus and reovirus_, nor will they kill all bacteria types.

The three common bacteria we will be testing against are: Pseudomonas aeruginosa - An ubiquitous environmental organism associated with whirlpools whereby infection is caused by immersion in contaminated water. Staphylococcus aureus - A normal flora organism on the skin surfaces of most healthy humans. Escherichia coli – A large and diverse group of

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