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Diabetic Foot Ulcer Research Paper

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Diabetic foot ulcer (DFU) is a major complication of diabetes mellitus [2]. Non-healing ulceration usually progress to amputation in spite of standard care. DFUs result from interaction of ;neuropathy, peripheral vascular disease and foot deformity. 80% of diabetic persons with foot ulcers present with peripheral neuropathy which is the greatest risk of foot ulceration [3]. Neuropathy in DM shows variable degrees of autonomic, sensory, and motor functions changes[4]. Motor neuropathy causes atrophy of the foot muscles resulting in clawing of the toes, sensory neuropathy results in repetitive trauma to the foot, and autonomic neuropathy changes skin consistency so accelerating ulcer formation [5] .40 to 50% of all patients show ischemic manifestations with the most having neuroischemic ulcers, and only few of them has purely ischemic …show more content…

Materials and methods This study was conducted on 30 subjects: 15 with diabetic foot ulcers and 15 with varicose vein ulcers as a comparison group. The study protocol was done according to the local ethics committee of the Menoufia University. Informed consents were taken from the subjects before the start of the study. For all subjects, the followings were done: history taking and clinical examination, clinical evaluation of the ulcers, complete blood count, preparation of platelet rich plasma (PRP) and quantification of Platelet count in it, preparation& application of PRP gel on ulcers, quantification of platelet derived growth factor (PDGF-AB) in PRP prepared before and after activation ,using CaCl2, by sandwich-based enzyme-linked immune-sorbent assay (ELISA) (Ray Biotech – USA)

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