Essay about Dialysis Lab Report

inside of the bag, and starch and water in the beaker? Why? The starch would stay in the beaker and the glucose and Lugol’s solution would be inside and outside of the bag. The glucose would then able to diffuse because the molecules are now small enough to pass through it.

If the solution in the left beaker contained both urea and albumin, which membrane(s) could you choose to selectively remove the urea from the solution in the left beaker? How would you carry out this experiment?

A. The size of the molecule. The larger molecule will diffuse more slowly than the smaller molecule.

In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.

With all solutes set at a concentration of 5.00 mg/ml and the MWCO set at 20, filtration stopped at 60 minutes, and the projected completion was 100 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentrations for all solutes was 0.00 mg/ml. With all solutes set at a concentration of 5.00 mg/m and the MWCO set at 50, the filtration completed in 40 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentration for NaCl was 4.81 mg/ml, and 0.00 mg/ml for all remaining

My prediction for the effect Na+Cl- might have on glucose transport was that the glucose transport rate would decrease. I picked this choice because I thought having Na+Cl- in one beaker would limit the space needed for proper glucose diffusion. All of the other runs involved water, so I predicted that adding a new solute could slow diffusion. The results opposed my

We hypothesize that as the solute concentration increases, more water will diffuse into the dialysis tubing (shown by a greater percent increase in mass).

Table 1 shows the contents of the bags and the content of the concentration it was submersed in. Bags 2-4 each contain a solution of both sucrose and water. These bags were each put into beakers containing hypertonic solution. These bags gained weight over time because the water moved from its high concentration inside the beaker to the low concentration inside the membrane of the artificial cell, the membrane being the bags that consisted of dialysis tubing. The

If there is more NA it the ATPase will have trouble pumping correctly causing problems in the kidneys

Using the graduated cylinder, measure 20mLs of the stock sucrose solution and 180mL of water to create a 3% sucrose solution and place it into the 250mL beaker (beaker #2). Place bags #1‐3 (red, blue, yellow) into beaker 2 and bag #4 (green) into beaker 1. Allow the bags to sit for one hour. After allowing the bags to sit for one hour, remove them from the beakers carefully open the bags, noting that often times the tops may need to be cut as they tend to dry out. Measure the solution volumes of each dialysis bag using the empty 250 ml beaker.

The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.

Objective(s): The reason for this experiment is to see how starch and iodine affect each other and how a plastic bag works similar to a membrane in certain situations.

One dialysis tube was submerged in each beaker. Osmosis was allowed to occur for 5 minutes and then all of the tubes were removed from the water. The tubes were dried off and measured on the triple beam scale. The mass was taken and recorded for all three tubes. I then placed the tubes back into their respective solutions. The process was repeated four times for each tube in 5 minute increments, and then the materials were disposed of. The rate of diffusion of water in each solution was

The Congo red solution is used to mimic blood and the yellow food color mimics the excretory product of the kidney. When a mixture of Congo red, yellow food color and water were mixed and taken in a dialysis bag, the yellow food color diffuses out into the surrounding water in which the dialysis bag is suspended. At the end of the experiment, the contents in the dialysis bag represent the blood; the contents of the beaker represent the urine formed. The beaker has a slight yellow tinge that results from the yellow food color.

The beaker was then filled partially with distilled water; 1 ml of potassium iodide was then added, and the solution was tested for the presence of glucose. This data was recorded in table 1 on the data sheet along with the starting color of both the potassium iodide solution and the glucose/starch solution. The dialysis tubing was then submersed into the beaker containing the potassium iodide solution, and set aside for 30 minutes to allow maximum diffusion.