Dna And Tissue Extraction Kit Essay

Better Essays

In this study we extensively extracted deoxyribonucleic acid (DNA) from an unidentified segment of flesh distributed amongst different lab groups. In order to do this, we dissolved the tissues and extracted only the genetic material desired using the Qiagen DNeasy Blood & Tissue extraction kit. We then used forensic methods of genome identification in order to determine the genus and species of the animal These methods include gel electrophoresis, Polymerase Chain Reaction, and Cycle Sequencing Reactions. After isolating the DNA, we gathered electropherograms from the CSRs to determine the nitrogenous base frequencies of the extracted sample using a genome alignment software program called GENEIOUS. Using this aligned data, we compared the results to the online genome index GenBank using the Basic Local Alignment Search Tool (BLAST).
After analyzing the results of the alignment programs and the genome index, I determined the genus and species of my group’s sample as being Crotalus durissus, a pit-viper located in Southern and Central America. This data was determined with an E-value of 2 x 10^-5 and was taken from the mitochondrial gene with a 97% accuracy match out of 187 nitrogenous base groups.

Introduction: Deoxyribonucleic acid, or DNA, is the polymer of nucleic acids that make up the foundation of all life. It is the necessary code from which an organism is derived, including all amino acids, anatomic structure, and even, in some cases, behavioral patterns.

Get Access