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Drosophila Fruit Fly Experiment

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Drosophila Project Report Sepia (eyes) x wildtype (WT) German Lagunas-Robles Marie Villalobos Jon Sacro December 4, 2014 I. Introduction The fruit fly (Drosophila melanogaster) is the model organism when it comes to studying genetics; for over 100 years, it has been used as one of the few model organisms in the scientific community (Twyman, 2002). The reason behind this and why scientists continue to choose to study Drosophila is because of the amount of scientists that have done so and it is well documented on how to treat a fly in a laboratory setting (Twyman, 2002). They are also small and easy to feed. This means that it would be easy to work with a large amount of them without being concerned about taking up too much space, food, or …show more content…

Methods 1. Two clean vials were obtained and labeled “P – Generation: Sepia x WT.” Fly food mixture was placed inside the vial and water was added to it until it took on a dark blue color. If too much water was added, this step was redone as the food could possibly drown the flies inside. 2. Six Sepias (3 males, 3 females) were added to both vials. Six Wildtypes (3 males, 3 females) were added to both vials. This was done by transferring them while they were FlyNapped. They were topped immediately after with the vial being placed on its side so the flies would not drown in their food when they woke up. These vials contained the parental generation. 3. After a week, the flies that resulted from P – generation, F1, were FlyNapped and taken out by placing them on index cards. Paintbrushes were used to transfer them onto the index cards. They were sorted into the following categories – male Sepia, female Sepia, male Wildtype, female Wild Type – and counted, this was F1. Six Sepias (3 males, 3 females) and six Wildtypes (3 males, 3 females) were then added to two separate vials. The vials were placed on their sides to avoid any flies drowning. The flies remaining were disposed of properly (fly

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