Drosophila Project Report Sepia (eyes) x wildtype (WT) German Lagunas-Robles Marie Villalobos Jon Sacro December 4, 2014 I. Introduction The fruit fly (Drosophila melanogaster) is the model organism when it comes to studying genetics; for over 100 years, it has been used as one of the few model organisms in the scientific community (Twyman, 2002). The reason behind this and why scientists continue to choose to study Drosophila is because of the amount of scientists that have done so and it is well documented on how to treat a fly in a laboratory setting (Twyman, 2002). They are also small and easy to feed. This means that it would be easy to work with a large amount of them without being concerned about taking up too much space, food, or …show more content…
Methods 1. Two clean vials were obtained and labeled “P – Generation: Sepia x WT.” Fly food mixture was placed inside the vial and water was added to it until it took on a dark blue color. If too much water was added, this step was redone as the food could possibly drown the flies inside. 2. Six Sepias (3 males, 3 females) were added to both vials. Six Wildtypes (3 males, 3 females) were added to both vials. This was done by transferring them while they were FlyNapped. They were topped immediately after with the vial being placed on its side so the flies would not drown in their food when they woke up. These vials contained the parental generation. 3. After a week, the flies that resulted from P – generation, F1, were FlyNapped and taken out by placing them on index cards. Paintbrushes were used to transfer them onto the index cards. They were sorted into the following categories – male Sepia, female Sepia, male Wildtype, female Wild Type – and counted, this was F1. Six Sepias (3 males, 3 females) and six Wildtypes (3 males, 3 females) were then added to two separate vials. The vials were placed on their sides to avoid any flies drowning. The flies remaining were disposed of properly (fly
It was decided that there would be 80 vestigial flies and 20 wild type flies to total to an initial population of 100 drosophila. Next, the flies were anesthetized flies using Fly Nap. The flies were counted out to reach desired ratio, sexing the flies making sure there are equal amounts of males and females to be sure there is ample individuals to allow successful mating. The fly’s food was prepared by taking a frozen rotten banana, cutting it in half, mashing up the banana meat, and mixing yeast into it. The
Introduction: The intention of this lab was to gain a better understanding of Mendelian genetics and inheritance patterns of the drosophila fruit fly. This was tasked through inspecting phenotypes present in the dihybrid crosses performed on the flies. An experimental virtual fly lab assignment was also used to analyze the inheritance patterns. Specifically, the purpose of our drosophila crosses is to establish which phenotypes are dominant/recessive, if the traits are inherited through autosome or sex chromosomes and whether independent assortment or linkage is responsible for the expressed traits.
Now mate a mutant F1 female fly with a mutant F1 male fly. Out of the 50 F2 progeny, what percentage of flies are wild type and what percentage are mutant
Steps 7-11 were repeated to record the phenotype of the F2 generation and number of male or female flies.
Table 2 shows the phenotypes of the F1 flies produced by crossing P1 wild-type males and P1 no-winged females. The results of that cross was that there was fifty nine wild-type females and forty one males. Therefore there was a total of one hundred wild-type flies produced from crossing P1 true breeding wild-type males and P1 true breeding virgin
21) In the next few days, continue to anesthetize and count the F₁ generation flies in the original vial.
A. The flies in the rural boxes were devoured by its predator (Spalangia), these small wasps, lay eggs into immature fly pupae after hatching, the wasp larvae feeds on the developing fly, killing it. This study suggests that stable flies are vulnerable out in the rural pastures rather than inside the feed lots where
In the BioOne Research article, Researcher have examined different fruits and vegetables acidics. The researchers collected fruits and vegetables to see how many were or not contained with larvae. With a fruit that have a strong acidic cause the sample to have less amount of larvae than the ones that did not have a high amount of acidic. As there result, researchers found that the citrus peels such as lemon or tangerine can reduce the amount of infested Queensland Fruit Fly larvae. At the table of the next page, you will see the classification of the Bactrocera Tryoni provided by National Center for Biotechnology Information (NCBI) and Integrated Taxonomic Information System
The idea of the project was to experiment breeding Drosophila Melanogaster (fruit fly) to figure out if certain genes of that species were sex linked or not (autosomal). A mono-hybrid cross and di-hybrid cross was performed. For the mono-hybrid cross, white eyed female and red eyed male were placed in one vial for them to reproduce. For the di-hybrid cross, red eyed and normal winged flies and sepia eyed and vestigial winged flies were placed in their vial to reproduce. In the mono-hybrid cross the results expected were within a 1:1:1:1 ratio. Expected results similar to the expected desired null hypothesis proposed with what the F1 parental generation breeds. The potential results would have had
Reproduction is a result of fertilization which leads to cleavage of cells. Time elapsed regarding reproduction varies from species to species. Particularly for this experiment is Drosophila. Drosophila is a fruit fly that has a shorter reproduction rate compared to other species. On average it takes these fruit flies nine days to hatch and become a fully formed adult. Prior to reproduction is the mating technique which occurs based off a series of motions and noises. Males which appear to have dark abdominal pigmentation display mating behavior consisting of orienting, tapping, singing followed by licking.
House flies are commonly found around homes and businesses. Various method has been developed to attract and trapped these flies either by mechanical or chemical means but hygienic attractant for locally use is the main problems for Malaysian use. This research paper describes a comparative study between local and commercial on the best bait to attract house flies. The food industries, the fish market and chicken farm were selected for conducting the experiments for 6 days. Then, identifying the volatile compound for the most effective baits was determined. This was done by using chemical test, were characterized its active compound with gas chromatography-mass spectrometry (GC-MS; QP2010, AOC-205 Shimadzu Auto Sampler). The finding of the
Genetic Crosses In Drosophila melanogaster Edison Nurcellari, Norma Sutton, Lisa Brown Abstract: The purpose of this experiment is to see how characteristics such as ( phenotypes) are transmitted from parents to offsprings by using true breeding stocks. While doing more than two test crosses from the parent the alleles are re-shuffle so the offsprings get different variations sets of allele then the parents. This process is known as genetic recombination which occurs during meiosis were chromosomes break and exchange genetic material. Another cause of offspring to receive certain allele combination is also due to natural selection for survival and reproduction.
For our first generation (F1) of flies we chose to cross apterous (+) females and white-eye (w) males. We predicted that the mutation would be sex linked recessive. So if the female was the sex with the mutation then all females would be wild type heterozygous. Heterozygous is a term used when the two genes for a trait are opposite. The males would all be white eye since they only have one X chromosome. If the males were the sex that had the mutation then all the flies would be wild type but the females would be heterozygous.
The spotted wing Drosophila suzukii (Diptera: Drosophilidae) is an attacking pest of berries crops. Unlike most other Drosophila, this insect can oviposit into ripe and ripening berries, so that they make them unmarketable. D. suzukii is spreading quickly throughout the continental US and they give a serious damage in horticultural areas and the fruit industry. Spotted wing drosophila (SWD) puts its eggs in both commercial fruit and wild fruits, so it is of great concern to fruit and vegetable growers. It became a pest of economic significance, but it is important point that requires early detection, monitoring and moderation to control it. Last researches show that D. suzukii infests a number of wild plant species and also those may be harvested commercially like cranberry. Gray dogwood (Cornus racemose Lam.), Tatarian honeysuckle (Lonicera tatarica Linneaus), Mulberry (Morus alba L.), Nanking cherry (Prunus tomentosa L.), Common chokecherry (Prunus virginiana L.), Common buckthorn (Rhamnus cathartica R. Davurica), Elderberry (Sambucus Canadensis L.), White snowberry (Symphoricarpos albus L.), Yew (Taxus
Another problem that these flies cause is that they can hurt the population. Sometimes these flies can infect a majority of a village's