Effect of Sucrose Solution on Osmosis
Aim: The aim of the experiment is to show how varying the concentration of sucrose solution affects osmosis by changing different molar solutions of sucrose and water and how it affects the potato. Introduction: In this investigation I will be exploring the effect of varying concentration of sucrose sugar solution on the amount of activity between the solution and the potatoes.
Osmosis is the movement of water molecules across a partially permeable membrane from a high water concentration to a low water concentration. Osmosis can be effected different things such as:
- Temperature - The warmer the solution, the quicker the osmosis will go. This is because the water
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The potato cells, took in, or gave out the water depending on the concentration of the solution it is surrounded in.
The results were fine and by looking at the mass measured before the experiment, you can see that there is no reading which seems to be out of the line. As the weights before the experiment range between 2.31g and 2.46g, this tells us that the potato pieces were cut well, and I believe accurate enough.
The results show that:
- Osmosis actually took place in the experiment.
- As the sucrose solution increased, the mass decreased, and as the sucrose solution decreased, the mass increased
Evaluation: I followed the plan correctly; I believe I gained accurate and sufficient enough results to conclude the experiment, and to prove my prediction.
To make this experiment better, I believe that I could have done more than one experiments to see if anything has gone wrong, but since
This data was analyzed by calculating the cumulative change in weight for each dialysis bag. This was done from subtracting the weight of each bag from the initial weight of the bag. Doing so, allows the weight of each bag to be initially zero. For that, we must calculate the corrected cumulative change in weight. For each time interval of 10 minutes, we subtracted the change in weigh of bag #1 (tap water) from the weight of each bag at the specific time measure- this corrected any oscillations.
My prediction is that as the concentration increases, the potato cell will lose more weight. This is because of the osmosis of water particles from the potato cell cytoplasm to the solution, resulting in a loss of weight. As the concentration decreases, the potato will lose less weight until a certain point where the osmosis of particles in and out of the potato cells will be equal. I also predict that as the salt
This experiment gave a visual understanding of osmosis and diffusion. The first experiment proved that solutes would move down a concentration gradient if permeable to the selective membrane. The second experiment proved different solute concentrations affect the movement of water, depending on the solute concentration inside the cell. The purpose of this lab was to look for different solutes that can cross an artificial membrane and to observe the effect of different concentrations of sucrose on the mass of a potato cell. Results for Part One suggested that the molecular weight of albumin and starch was too large to
As the Concentration of the Sucrose Solution decreases, the more the potato’s mass increases. This is due to the solution being hypertonic. So, as the solute concentration gets lower, the potato’s water concentration will get higher, therefore more water particles from the solution will absorbed by the potato. Some changed very little in mass because the concentrations of the H2O molecules in the potato and outside the potato were equal. This equality in concentration is called Isotonic.
Osmosis is the diffusion of water across a membrane to create an equilibrium between the levels of concentration of a solute both inside and outside the cell. In this case the solute will be sugar as the potato core will be immersed in sucrose solution.
The percent change in mass decreased as the sucrose concentration increased (Figure 1). The percent change in mass decreased as sucrose concentration increased, therefore, relative osmotic concentration also decreased as sucrose concentration increased. The osmotic concentration was greater than zero in sucrose solutions of 0.0. The osmotic concentrations were less than zero in sucrose solutions of 0.1, 0.4, and 0.5 M. The osmotic concentrations were zero in sucrose solutions of 0.2 and 0.3 M. At sucrose concentration 0.1 M the change was negative which does not fit the trend line, but if the experiment was repeated a few times that would probably change. The percent changes in mass from 0 to 0.5 M was very dramatic.
Repeated Trials: In procedure one, we tested diffusion on different sized cell models (gelatin with various volume and surface area wise). In procedure two, we tested cell models in different internal environments and similar external environments to find the effects on rate of osmosis. In procedure three, we used potato cells in different concentrated sucrose environments to test the effects on water potential on cells and osmosis.
During osmosis, solvents move across a semipermeable membrane in order to regulate the solute balance within the cell (Campbell Biology). Experiment 5.5 was conducted to further research osmotic activity, particularly in potato cells in different osmolarities of a NaCl solution. The goal of the experiment was to find out whether the potato slices used would be hypotonic or hypertonic to the different osmolarities. This process is relevant because without osmosis, the passage of solvents would not be possible. To perform the experiment, seven potato slices were soaked in 5cm of a different osmolarity level of a NaCl solution (0M – 0.6M) to determine whether each slice was hypotonic or hypertonic to the NaCl solution it was placed in, based on percent weight change. The prediction that the potato slices soaked in solutions with lesser osmolarities (0M – 0.1M) of NaCl would be hypotonic to their solutions, and the potato slices soaked in solutions with higher osmolarities (0.2M – 0.6M) would be hypertonic to their solutions was supported by this experiment because the slices soaked in (0.2M – 0.6M) had
The reasoning behind this experiment is the examine whether the rate of osmosis is changed due to a change in temperature. It was hypothesized that the rate of osmosis will increase as the temperature of the sucrose is increased. The rate of osmosis was tested by using the different jars full of different temperate water and testing how high the water rose on an osmometer over a span of 20 minutes. An osmometer is a tool used to measure rates of osmosis. The different temperatures tested on a sucrose solution were 5 degrees Celsius, 20 degrees Celsius, and 37 degrees Celsius. Rates of osmosis were higher in the hot water than in the cold water and control. The results showed that the rate of osmosis increased as the temperature increased, henceforth the hypothesis was supported. In conclusion, the experiment showed how changes in temperature affect the rate of osmosis.
The aim of this experiment was to test the effect of surface area on osmosis and the effect of solute concentration on osmosis. To test this aim to hypotheses were devised. 1) If potato pieces are immersed in various salt solutions, then the pieces with the greatest surface area to volume ratio will experience the greatest weight change, because more water can move by osmosis across the potato cell’s semi permeable membrane. 2) If potato pieces are immersed in various salt solutions, then the pieces immersed in the most concentrated solution will experience he greatest weight change,
Van’t Hoff’s Law suggests that the osmotic potential of a cell is proportional to the concentration of solute particles in a solution. The purpose of this experiment was to determine if there are any differences between the osmolalities, the no-weight-changes of osmolalities, and the water potentials of potato cores in different solutions of different solutes. The percent weight change of the potato cores was calculated through a “change in weight” method. The potato core’s weight was measured before and after they were put into different concentrations of a solute for 1.5 hours. In our experiment, there were no significant differences from the osmotic potentials of our results and the osmotic potentials of other scientists work. Ending with chi square values of 2.17 and 2.71, and p values of 0.256 and 0.337, concluding that there is no difference in water potentials of potato cores in different solutions of different solutes at varying concentrations.
The purpose of this experiment was to investigate the effect of the change in concentration of sucrose on the rate of osmosis in cylinders of potatoes.
The rate of osmosis increases as the concentration of the dissolved sugars in the solution increases. By increasing the concentration of the dissolved sugars within the solution, the water potential begins to increase in one area, causing osmosis to take place in order to balance out the concentration of water. Materials & Method Create sugar solutions with varying sugar concentrations (5%,10%,15%,20%,25% and 30%). Received a piece of membrane from a demmy and kept it wet. The membrane was used to cover the mouth of the funnel and use and secured it in place with an elastic band.
Data: Effect of Solute Concentration on Osmosis in Potato Cells (for the 6 groups of our class)
First I gather all my materials needed for the experiment (see materials list). Second I peel and slice potatoes weigh each potato in grams. Get these slices to weigh the same in mass because if they vary to much in mass that could affect the results of the experiment. Third I place each potato in its own beaker. Fourth once the potatoes are placed in beakers I make sure to place enough solution to fully cover the potato. Each beaker should have a different amount of