Osmosis And Diffusion Lab Report

4. Does the dialysis bag or the beaker contain more starch? What about glucose? The dialysis bag does contain more of the glucose and starch than the beaker does.

Using the graduated cylinder, measure 20mLs of the stock sucrose solution and 180mL of water to create a 3% sucrose solution and place it into the 250mL beaker (beaker #2). Place bags #1‐3 (red, blue, yellow) into beaker 2 and bag #4 (green) into beaker 1. Allow the bags to sit for one hour. After allowing the bags to sit for one hour, remove them from the beakers carefully open the bags, noting that often times the tops may need to be cut as they tend to dry out. Measure the solution volumes of each dialysis bag using the empty 250 ml beaker.

First, we collected the materials that were needed: 3 beakers, 2 clips, glucose test strips, cooked starch, amylase, and iodine. Then we put 4 pipettes of cooked starch in the small beaker. We then mixed the starch wit h4 pipettes of amylase. The starch will be the leaf; the amylase will be the digestive enzyme. The small beaker represents the head and crop. We then put 4 pipettes of the “macerated” solution from the crop to the soaked dialysis tubing. We filled the large beaker two-thirds full of water and put 4 drops of Lugol’s to the beaker. We then put the dialysis tubing into the large beaker. We recorded data

The Congo red solution is used to mimic blood and the yellow food color mimics the excretory product of the kidney. When a mixture of Congo red, yellow food color and water were mixed and taken in a dialysis bag, the yellow food color diffuses out into the surrounding water in which the dialysis bag is suspended. At the end of the experiment, the contents in the dialysis bag represent the blood; the contents of the beaker represent the urine formed. The beaker has a slight yellow tinge that results from the yellow food color.

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

The dialysis bag experiment resulted with beaker one having relatively similar numbers, showing as an isotonic medium. Beaker two’s solution gradually increased within an hour which caused the product to

The purpose of this experiment was to examine the permeability of the dialysis tubing using molecules of various sizes (iodine, water, glucose, and starch) and reagents to help identify the molecules in the solution. It was assumed that iodine, then water, followed by glucose and starch respectively, would have the most ease of movement, as they are in the order of size (least to greatest). Being an element, iodine is the most minuscule out of the four, as they are the building blocks for molecules, such as: water (H2O). As a result, iodine was able to move with ease through the dialysis tube from the beaker with water, which was indicated by the tints of black and purple in the dialysis tube, as a result of the reaction between this element and starch. Consisting of molecular compounds, carbohydrates are created with the three elements, carbon, hydrogen, and oxygen in a one to two to one ratio.

The independent variable was the concentration of sucrose in the dialysis tubing we used as a simulated membrane.

The hypothesis states that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant. In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution  made during the study,  four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis.

With all solutes set at a concentration of 5.00 mg/ml and the MWCO set at 20, filtration stopped at 60 minutes, and the projected completion was 100 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentrations for all solutes was 0.00 mg/ml. With all solutes set at a concentration of 5.00 mg/m and the MWCO set at 50, the filtration completed in 40 minutes. The residue analysis indicated all solutes present in the dialysis membrane. The filtrate concentration for NaCl was 4.81 mg/ml, and 0.00 mg/ml for all remaining

When talking about dialysis, it is most often associated with the function or failure of the kidneys ability to process and rid the body of toxins from the blood. It would prove to be fatal if the kidneys failed to remove the roughly 1,500 liters of waste building up in the body (Nordqvist, 2015). This experiment mimics the nature of a selective plasma membrane and with time, monitors to passing of substances in and out of the barrier. The end results should appear to show a color changing chemical reaction within the more concentrated tube of 15%glucose/1%starch solution. Initially the clear and milky appearance should transform into a black and deep purple hue from the attachment of iodine to the sugar solution.

Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is

There were several steps completed to prepare for the experiment. Three dialysis tubes were filled with approximately the same volume of distilled water and then were tied shut. The initial mass (in grams) of the tubes was taken using a triple beam scale. I then filled three 500 mL beakers with 400 mL of water each and dissolved different masses of solute (table sugar) in each beaker in order to make 5%, 10%, and 20% solutions. The beakers were labeled accordingly, and then 20 g, 40 g, and 80 g (respectively) of table sugar was weighed out using a digital scale and placed into the corresponding beakers. The sugar was stirred in using a stirring rod until all of the solute was completely dissolved.

Osmosis is the movement of water molecules from high concentration to low concentration through semipermeable membranes, caused by the difference in concentrations on the two sides of a membrane (Rbowen, L.). It occurs in both animals and plants cells. In human bodies, the process of osmosis is primarily found in the kidneys, in the glomerulus. In plants, osmosis is carried out everywhere within the cells of the plant (World Book, 1997). This can be shown by an experiment with potato and glucose/salt solution. The experiment requires putting a piece (or more) of potatoes into glucose or salt solution to see the result of osmosis (a hypertonic type of solution is mostly used as it would give the most prominent visual prove of

To conduct this experiment we used four pieces of dialysis tubing, four pieces of string, one beaker filled with 10% sucrose and another beaker filled