Effect of Temperature on an Enzyme Controlled Reaction

3764 Words Jun 26th, 2006 16 Pages
To investigate the effect of temperature on an enzyme controlled reaction

Introduction and planning

For the investigation of enzymes, I am going to conduct an experiment to see how temperature can affect the rate of reaction of enzymes by testing it with starch.
The enzyme that we are going to use is called amylase. We are going to test this enzyme with starch. By mixing amylase and starch solutions together under different temperature conditions, we can record the rate of reaction by taking a sample out and test it with iodine solution to see if there is any remaining starch present.
We have to use the enzyme amylase because enzymes will only work on a specific substrate i.e. amylase will work on starch because of its special shape of
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The rate of reaction did not start at the point zero, it started around 3 and reached its optimum at around pH 7 to 8. It started to decrease and finally there were no reactions at around pH 11 to 12. Concentration of enzymes
If the concentration of enzymes increases, there is an increase of enzyme molecules and active sites available. This gives a higher chance of collision with a substrate and forms a product.
The graph should look like this:

The rate of reaction is directly proportional to the enzyme concentration.
As long as there is enough substrate available to bind with an active site, the rate of reaction will increase linearly with enzyme concentration.

Concentration of substrate
As the concentration of substrate increases, the rate of reaction will also increase because the substrates are binding with enzymes. At the beginning of the reaction, the line increases steeply because there is less substrate compared to the concentration of enzymes. They will bind with an enzyme very quickly, resulting in a steep linear at the beginning. But as soon as the amount of substrate increases to a certain point (saturation point), the rate of reaction starts to flatten out. This is because there aren't enough enzymes free for binding.
It will always get to a point where all the enzymes are being used and adding more substrate
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