Abstract: The purpose of this experiment was extract proteins from the muscle tissue of various fish species and execute an SDS-PAGE to determine similarities in their respective protein profiles. The seven species examined in this investigation were the Yellow Tuna (Thunnus albacares), Thresher Shark (Alopias vulpinus), Paiche (Arapaima gigas), Swordfish (Xiphias gladius), Catfish (Ameiurus catus), Coho Salmon (Oncorhynchus kisutch), and Rainbow Trout (Oncorhynchus mykiss). A sample of muscle tissue from each of the seven fish species was placed in a microtube containing Laemmli Sample Buffer, in order to disrupt and denature the tissue. The microtube was incubated at room temperature for five minutes, and then was placed in a 95°C hot …show more content…
They are directly controlled by DNA specific sequencing and coding. Since proteins are influenced by DNA, their profiles, also known as proteomes, are indicative of evolutionary and genetic relatedness. These proteomes vary from cell to cell, as the function of each group of cells is different. Proteomics refers to the study of protein structure, function, and interaction within a certain environment in an organism.
The purpose of this experiment is to perform a procedure known as SDS-PAGE (sodium dodecyl sulfate - polyacrylamide gel electrophoresis) in order to determine protein profiles for seven fish species: Yellow Tuna (Thunnus albacares), Thresher Shark (Alopias vulpinus), Paiche (Arapaima gigas), Swordfish (Xiphias gladius), Catfish (Ameiurus catus), Coho Salmon (Oncorhynchus kisutch), and Rainbow Trout (Oncorhynchus mykiss). The gel electrophoresis separates the fish muscle proteins according to their molecular weights, which allows one to determine a profile for each specie.
The goal of this experiment is to extract proteins from fish muscle tissue, unfold and denature them, load them for gel electrophoresis, and determine the success in our efforts to classify and compare fish muscle protein profiles. These protein profiles will help establish the shared characteristics and evolutionary relationships between any two given fish species.
Materials and Methods: In order to investigate
In a previous study, researchers Ellerby and colleagues (2001) examines the fast muscle in Anguilla anguilla during aquatic and terrestrial locomotion. Ellerby et al. (2001) noticed that the stimulus patterns in the muscles that are linked to swimming create a stronger force and power than terrestrial crawling. The reason there is a less muscle performance on land is due to the possibility the eel is restricted from a particular kinematic pattern that is needed to push from a
The fish are not available in California due to state regulations that ban biotech aquatic organisms (GloFish, 2011). In addition, Singapore – where the fish was originally engineered – has been reluctant to approve its sale (Ely, 2004). This places an additional limit on potential marketing and distribution strategies.
Tachylectin-5A is a protein found in the immune system of the Japanese horseshoe crab, Tachypleus tridentatus. Tachylectin-5A, which has a PBD reference number of 1JC9, is mainly found in the hemolymph plasma of horseshoe crabs (1). This protein was determined on June 8th, 2001 via x-ray diffraction (1). The X-ray diffraction results in an absorbance of 2.01 Å (2). It shows that the protein contains 269 residues, and the molecular weight ot is 380-400 kDa (2). Proteins similar to tachylectin-5A have been found in various organisms like slime molds, sponges, bony fish, and lancelets (3). Tachylectin-5A is structurally related to the fibrinogen γ fragment, which is found in mammals’ plasma; and it is similar to a protein in humans called ficolin, which is involved in host defense through nonself-recognition (4).
A way to look at the muscle tissue of the A. jubatus is to look at the fiber composition of the muscles and categorize them. By identifying the fiber composition we will be able to investigate what each muscle fiber does and what its action is. It is known that skeletal muscles contain different types of muscle fibers (Burke, 1981). Muscle fibers can be classified into Type I, Type IIa, Type IIb, and Type IIx by staining them with monoclonal antibody for each myosin heavy chain isoform and metabolic enzyme activities (Pette and Staron, 1993, 1997)
The Bluefin Tuna possesses an elongated streamlined body which contains small to moderate sized scales throughout. The upper exterior is metallic blue and the lower exterior is silver in colour, with gold coruscation distributed sparingly around the body. In humans, their bodies are covered in skin which is the largest organ within the human body (Ebling and Montagna, 2017). The skeleton of the Bluefin tuna comprises of the vertebral column, jaw, ribs, cranium and intramuscular bones. ("Skeletal System Of Fish | Meaning Of Fish Skeleton Necklace", 2017). The skeletal system of the Bluefin tuna provides structure, provides protection, assists in leverage and assists in the production of red blood cells, in conjunction with the kidneys and spleen
Australian freshwater crayfish, Cherax cainii is an important cultured species in Western Australia. As a part of fast growing aquaculture activity, future aquaculture of this species will be also affected by the limited supply of fishmeal (Olsen and Hasan 2012). Fishmeal has been used as protein source mainly in aquaculture, poultry feed, and pig (Tacon and Metian 2009). Fishmeal has very complex amino acids characteristic which is required by the fish to grow (Wilson and Halver 1986). Lack of amino acids in the feed lead to health problems in cultured fishes (Kloppel and Post 1975, Poston and Rumsey 1983, Halver and Shanks 1960). However, the increasing trends of fishmeal utilization in last two decades (Tacon and Metian 2009) combine with the stagnation of wild caught fisheries (FAO 2014) become a major pressure on the fishmeal supply. Thereof an alternatives fishmeal substitute is required to
The main focuses of this book are the biology of sharks, types of sharks, and threats to sharks and conservation. In “Shark Biology,” they show you the what each part of the shark’s body is used for. Then in “Types of Sharks,” they tell you
Fish is on top of the list of foods that build muscle. It is a high source of protein, but what also puts it on top of the list is the richness of omega 3 fatty acids. A lot of people think that all fat is bad for the health, which is simply not true. It is saturated fat that we want to avoid which is also avoided by many to be put on the list of foods that build muscle. The nutritions in fish also has a lot of internal health benefits.
The little tunny, Euthynnus alletteratus, is a common species of tuna and a popular sport fish inhabiting the Atlantic. Relatively small in size, the little tunny may grow to be a meter in length, although they are often much smaller. This species can weigh up to 12 kg, but, as stated with their length, tend to be much smaller (Robins, 1986). Similar to other species of tuna, the little tunny has a fusiform body shape that allows for efficient movement through water and they lack a swimbladder. They also possess a keel along each side of their narrow
10a). At 12 weeks transgenic channel catfish containing channel catfish Ictalurus punctatus growth hormone (ccGH) cDNA, driven by the ocean pout Zoarces americanus antifreeze protein promoter (opAFP), opAFP-ccGH, grew 1.4 to 1.6 fold larger than their non-transgenic siblings, and transgenic channel catfish containing channel catfish growth hormone (ccGH) cDNA, driven by the rainbow trout Oncorhynchus mykiss metallothionein promoter (rtMT), rtMT-ccGH, grew 1.35 to 1.8 fold larger than their non-transgenic siblings (Table 2; Fig. 10a) (P < 0.0001). However, there is no significant between transgenic of opAFP-ccGH family number 1 and non-transgenic rtMT-ccGH family number 2, and no significant between transgenic of rtMT-ccGH family number 1 and non-transgenic opAFP-ccGH family number 2 (Fig. 10a), probably due to the family effect. There is always a significant difference between mean body length of transgenesis and their non-transgenic siblings (Table 3; Fig. 10b). However, there is no significant difference between transgenic of rtMT-ccGH family number 1 and non-transgenic rtMT-ccGH family number 2, and non-transgenic of both families of opAFP-ccGH (Fig. 10b). The body weight and length were highly correlated (P < 0.01) in both genotypes in both families. Our results indicated that to develop a fast growing channel catfish we need combine the transgenic ccGH by
Researchers has sequence cichlid fish in order to give a better understanding on the evolution, development, neurology , ecology, physiology, and agriculture of the fish around the world.
Question 3: Tuna and Cod will be the most related animals. They both swim and migrate in large schools and can be found in the Pacific Ocean. They both have multiple dorsal fins and can be found in a deeper level of the ocean. This indicates that their protein profiles could be similar.
The population of Bluefin Tuna has been dramatically declining over the past few decades with no expectation of slowing down. Bluefin Tuna are a very important species of fish because they are one of the top predators in the ocean and if they become extinct the balance of the ocean’s ecosystem would be off. The classification of Bluefin Tuna includes a few different types, all of which are endangered and it is estimated that they will soon become extinct. The categorization of each type of Bluefin Tuna species is based on geographical location although the exact number varies from 3-8. Although there are still significant number of Bluefin tuna in the ocean, the demand for the fish has increased dramatically with no sign of decreasing. This demand has caused new and harmful fishing methods to be created. They have become incredibly popular in sushi as well as other food and for this reason the population has declined; however, there are methods that could be used in order to prevent the species from becoming extinct.
Proteomics is the study of proteins’ structure and functions of proteins produced by an organism at a cellular level. Proteomics includes variations made to the proteins as well as interacting partners and
As the world more focus and advance forward on building a better self (as a person), such as healthy diet, people consume a lot of fish in such pursue. Among all the fish Salmon and Tuna which are rich in protein. Matter of a fact according to “RAINCOAST TRADING” consumers sustainably-harvested gourmet canned seafood company nutrition fact state, same amount of canned Salmon 55g and Tuna 55g, tuna has cal: 90, protein: 17g while salmon has cal: 110, and protein: 13g. So, lot of people favor over Salmon and consume more Tuna. But the problem is how a lot of Seafood Company caught or got the tuna which is harmful fishing practices instead of traditional methods and growing global high demand for seafood.