Abstract: The purpose of this experiment was extract proteins from the muscle tissue of various fish species and execute an SDS-PAGE to determine similarities in their respective protein profiles. The seven species examined in this investigation were the Yellow Tuna (Thunnus albacares), Thresher Shark (Alopias vulpinus), Paiche (Arapaima gigas), Swordfish (Xiphias gladius), Catfish (Ameiurus catus), Coho Salmon (Oncorhynchus kisutch), and Rainbow Trout (Oncorhynchus mykiss). A sample of muscle tissue from each of the seven fish species was placed in a microtube containing Laemmli Sample Buffer, in order to disrupt and denature the tissue. The microtube was incubated at room temperature for five minutes, and then was placed in a 95°C hot…show more content… They are directly controlled by DNA specific sequencing and coding. Since proteins are influenced by DNA, their profiles, also known as proteomes, are indicative of evolutionary and genetic relatedness. These proteomes vary from cell to cell, as the function of each group of cells is different. Proteomics refers to the study of protein structure, function, and interaction within a certain environment in an organism.
The purpose of this experiment is to perform a procedure known as SDS-PAGE (sodium dodecyl sulfate - polyacrylamide gel electrophoresis) in order to determine protein profiles for seven fish species: Yellow Tuna (Thunnus albacares), Thresher Shark (Alopias vulpinus), Paiche (Arapaima gigas), Swordfish (Xiphias gladius), Catfish (Ameiurus catus), Coho Salmon (Oncorhynchus kisutch), and Rainbow Trout (Oncorhynchus mykiss). The gel electrophoresis separates the fish muscle proteins according to their molecular weights, which allows one to determine a profile for each specie.
The goal of this experiment is to extract proteins from fish muscle tissue, unfold and denature them, load them for gel electrophoresis, and determine the success in our efforts to classify and compare fish muscle protein profiles. These protein profiles will help establish the shared characteristics and evolutionary relationships between any two given fish species.