Fruit Fly Lab Report

The lab handout provided by the instructor was used as a guideline to conduct this experiment. The only difference was the organism used and data collection period. For this experiment, pill bugs and crickets were utilized. Also, data was collected for a period of 12 minutes.

The purpose of our experiment was to observe the behavior of the pill bugs. We were trying to find exactly whether the pill bugs would be attracted or repelled by the chemicals. The chemicals used were vinegar, water, salt solution and a sugar

As an experiment I would place multiple kinds of cereal in front of a single mealworm, and record their reactions to each certain type of

It is possible that the temperature at which the flies were kept dropped significantly below 20 degrees Celsius; this could have caused the death of some of the files or slowed down their growth and reproduction rate. This would result in there being fewer flies. This problem could be overcome in further experiments by using a larger heating device with a more responsive thermostat to keep the Fly house within the recommended temperature range for D. melanogaster.

In part 2 of the experiment we are inducing RNAi through the process of feeding. To do so, a black pen is used to label the bottom of an OP50 seeded plate with the date and “wild type”. Another OP50 seeded plate is labeled with the date and “dpy-13”. A black pen is then used to label the bottom of the plate seeded with dpy-13 RNAi feeding strain with the date and “wild type”. Five L4-stage worms from the plate of wild type worms are then picked and moved to the OP50 seeded plate labeled “wild type”. Any eggs or young larvae that may have been accidentally transferred are to be picked off of the plate and then flamed in a Bunsen burner. The same method is used to move five L4 wild type worms to the plate seeded with the dpy-13 RNAi feeding strain, and once again to move five L4 dpy-13 worms to the OP50 seeded plate labeled “dpy-13”. These plates are then incubated upside down at 20˚C.

The parents are both homozygous. The homozygous dominant would represent the wild type. And the homozygous recessive would represent the other fly parent of a different strain. The F1 generation would consist of 100% Wild Type but they would all be heterozygous in carrying the recessive gene.

Firstly, eggs were collected from the eyes of Scomberomorus niphonius and disinfected in 1% sodium sulfite solution for 3 min, and subsequently in 3% Lysol brand disinfectant for 5 min. The disinfected eggs were then transferred to sterile vials to clone. Secondly, third stage larvae of Lucilia sericata were selected to be placed in 3.5% formalin for 5 min, 2% hydrogen peroxide solution for 3 min, and then 5% dilute hydrochloric acid solution for 5 min. After the two-step disinfection, the larvae remined vigorous. A hundred randomly selected larvae were proven to be aseptic by bacterial culture test.

Every 30 seconds their preferred position was documented. Once the three minutes were up and all the information was documented then the ten pill bugs were gently collected and put back in to their jars. These pill bugs were then traded with another tables pill bugs. The new pill bugs came from a sucrose and water chamber habitat. Again ten of these pill bugs were gently grabbed and placed in the chamber for 3 minutes with every 30 seconds documenting where the pill bugs preferred. After the 3 minutes the pill bugs were grabbed placed back in to their jars and switched again with a different table. The last trial of pill bugs came from a tea and water habitat. The ten pill bugs were gently grabbed and placed in to the chamber for three minutes. Every 30 seconds their preferred habitat was documented. When the 3 minutes were up the pill bugs were gently grabbed and placed back in to their jar. In this experiment the control was the distilled water, the dependent variable was the number of bugs in the habitat preferred, and the independent variable was the alcohol, and the water

The containers were plastic and 18 by 15 by 6 centimeters long. Prior to the experiment being performed, the crickets had spent a week in these residencies. Along with the crickets in the plastic containers, there was wet pieces of paper towel and a slice of carrot.

The pill bugs were moving more in hot environment than cold temperature and room temperature. The pill bugs made more turning in room temperature than cold and hot environment. The pill bugs made more rounds in room temperature than cold and hot environment. The hypothesis was that pill bugs will react more in moisture or humid environment that others. Based on our result, the hypothesis will be rejected. The reason why the result was not accurate can be due to limitation or errors during the experiment. The fact that the same pill bugs were used for the three trials can be considered as an error because bugs might be tired after the first trial. In future the experiment, different bugs should be used in each trial. Another error can be the condensation that occurred during the heating process. A wet paper was placed in the Petri dish. During the heating process, the water evaporated and since the petri dish was covered with led, the vapor is transformed to water, however, pill bugs do not live in water. In the future experiment, the petri dish should be kept open to avoid the

METHODS: In this experiment, the instructor provided us with 30 ebony individuals and 20 wild type individuals. In order to get an exact amount of each type, we anesthetized the flies and counted them off by gently using a fine point paint brush. Then all 50 Drosophila were put into a population cage which had a lid that had six holes for the centrifuge tubes. Two food tubes and four clean, empty tubes were added on the first day. Each food tube consisted of half a cup full of food mixed with 6-7 milliliters of water. This was the fly medium. The food should turn blue once the water is added. Each tube was labeled with a number and with the date. Every two to three days we added one more food tube until all 6 tubes contained the fly medium. After all 6 tubes were filled, the following days after we exchanged the first food tube with a new food tube. At the end of the experiment, we fed the flies with a total of 8 food tubes. Then the flies were anesthetized, again. At the end of this four week lab, the number of living ebony and wild

To make the vials, equal volumes of media flakes and water are added. A few grains of yeast is also added to inhibit the growth of fungus. No flies should be added until the media has settled for 1 minute. For this experiment, P generation flies were provided in order to be bred.

For the setup, we gathered our materials: 1-Gallon Plastic Jug, a jar, a mesh screen, Isopropanol, an Apparatus, scissors, a ruler, some tape, and a collected soil sample. First, we cut the bottom of the jug using scissors. Then, we poured two centimeters of isopropanol into the jar. Then, we placed the milk jug funnel’s spout in the open part of the jar. The jug is compared to the funnel, while the jar is the collection chamber where critters will be trapped in. After this, we bend the mesh screen so that it’s securely into the milk jug and forms a stable platform for the soil sample. Then, we put the soil sample into the funnel on the jar and tape the ruler to the handle of the funnel and to the side of the jar to ensure that the funnel remains

Some larva containing vials had hatched into flies. Counting of the flies began at this point. As flies started to grow, at different rates for each vial, with in the first seven days after all larva had hatched the flies were counted. The procedure was done according to theDrosophila manual (45-2620)

During the summer of my sophomore year, my bananas and apples in the kitchen were coated with crawling nuisances. Fruit flies were present here, fruit flies were present there, fruit flies were present everywhere. My kitchen was the epitome of a fruit fly hive.