DNA extraction in Watermelon and Bananas
Abstract During this experiment the researcher investigated the differing amounts of DNA present in bananas and watermelon. So that the researcher could do this, and accurately record the amounts, the researcher liquefied the fruits in a blender, added water and salt to them, and the added laundry detergent before using contact solution as an enzyme. Rubbing alcohol was then poured into the mixture and the DNA was extracted and put into a small container so that it could be weighed. This experiment gave the researcher the knowledge that bananas do in fact have more DNA than watermelon. The results were recorded in a chart and expressed through
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20. Put 6 ml of contact cleaning solution in each container. 21. Stir gently. 22. Tilting the container to the side, add a layer of rubbing alcohol. 24. Let the mixtures sit for 15-20 minutes, and watch the DNA rise to the surface. 25. Using the small strainer, separate the DNA from the rest of the solution. 26. Place the DNA in the plastic containers, the Banana DNA in one, and the Watermelon DNA in the other. 27. Weigh each on the gram scale. 28. Record the data.
Data
|Fruit |Weight of fruit |Weight of DNA |Fruit to DNA ratio |
|Banana |114g +-1g |18g +-1g |19:3 |
|Watermelon |114g +-1g |3g +-1g |19:1 |
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Conclusion While working on this experiment, the researcher was sure that the banana would have more DNA in it than the watermelon. In the end, this proved to be true with the banana getting 18 grams of DNA while the watermelon only got 3 grams of DNA, proving the hypothesis to be correct. The next time this experiment is attempted, the researcher would also like to test the difference of the amount of DNA in a bananas peel versus its
The next step consists of putting the strawberries into a sandwich bag and squishing in until all chunks disappear. Thirdly, sit a coffee filter on top of a cup and pour the mix into it and let sit until it stops dripping. For the fourth step, pour as much alcohol as there is strawberry mix into the cup . After a few seconds DNA will begin to rise to the surface. Take out the DNA using a spoon and place it in a measuring cup. For the next step the data must be recorded and the same steps should be completed with the other
Discoveries in DNA, cell biology, evolution, biotechnology have been among the major achievements in biology over the past 200 years with accelerated discoveries and insights over the last 50 years. Consider the progress we have made in these areas of human knowledge. Present at least three of the discoveries you find to be most important and describe their significance to society, health, and the culture of modern life.
This paper explores the history and some interesting facts about DNA. The last couple centuries have seen an exponential growth in our knowledge of DNA. The history of the DNA can be traced back to multiple devoted scientist. This article attempts to summarize, and review the basic history of DNA while providing some fascinating information about it.
In this lab, M&Ms and Smarties will be compared in five different ways: mass, shell solubility, volume, density, and nutrition facts. For each comparison 10 Smarties will be used and 10 M&Ms will be used. This is to ensure that the results are more accurate, as each piece of candy is not the same size, weight etc… A total of 50
23. The color, colony size, and overall size altered at the end of the experiment.
The research question of the article Designer DNA by Rachel Berkowitz was how the deoxyribonucleic acid (DNA) portrays a unique sequence of protein for every organism. Biologically, all animals including humans have a unique DNA. The study focuses on how the arrangement of four nucleotide bases determines the DNA sequence. For decades, scientists have altered the DNA of various organisms to manipulate the life of living things. They have inserted genes into algae, yeast cells, and bacteria to produce enzymes that appear in different shapes and structures. The researchers hypothesized that software makes it less tedious for scientists to predict the behaviors of host organisms whose DNA have been manipulated. In reality, the actions
The Material floating at the top of the test-tube after the alcohol was added was all the strands of DNA. The alcohol dissolved the cell walls of the cells and the DNA was free to move about freely.
DNA is the building block and root programing for every cell every living organism. DNA is a part of every cell in your body except for red blood cells. DNA has the single most important role in the development of every living cell in every living species on this planet. It dictates our lifespan, health, and what species we develop into. DNA should be considered the root deciding factor regarding the dietary needs of all species. This research paper will outline and define exactly how important DNA is to diet. As well as shine light on the fact that for the most part the relationship that DNA has on diet has not been applied to benefit the health of Human kind and the Animal Kingdom. I will also address how much taking DNA into consideration
Much can be learned from studying an organisms DNA. The first step to doing this is extracting DNA from cells. In this experiment, you will isolate DNA from the cells of fruit. Materials (1) 10 mL Graduated Cylinder(2) 100 mL Beakers15 cm Cheesecloth1 Resealable Bag1 Rubber Band (Large. Contains latex pleasewear gloves when handling if you have a latex allergy).Standing Test TubeWooden Stir StickFresh, Soft Fruit (e.g., Grapes, Strawberries, Banana, etc.) ScissorsDNA Extraction SolutionIce Cold EthanolYou Must ProvideContains sodium chloride, detergent and waterFor ice cold ethanol, store in the freezer 60 minutes before use. Procedure If you have not done so, prepare the ethanol by placing it in a freezer for approximately 60 minutes.
This report evaluate about the genetically modified food that why we need this, what are its key issues and how we can overcome these issues? In the field of biotechnology, the vast development was observed in the last few decades comprising the manipulation of genetic material to generate animal and plants with certain desirable attributes. Genetically Modified/Manipulated Organisms frequently termed as GMOs, refer to organisms whose genetic material has been modified through recombinant DNA (rDNA) technology, in which genes from the DNA of different organisms are recombined, having the both (positively and negatively) potential effect on the environment and human health. Recombinant GMOs can be generated by gene cloning techniques in which a non-native gene is inserted and expressed in a target organism. There are several methods behind the basic principle of recombinant DNA technology, but the most prominent method to is the inserted DNA from a donor organism into the target organism via a vector with cloned DNA. At present numerous GMOs as well as the products of GMOs are utilized by human and researches are persistently performing to transform the genetic traits of organisms to change for the better, according to human practices (Melo-Martín I & Zahra M, 2008). Genetic modification in plants can be inserted easily attributable to their ability to grow from a single cell or small pieces of tissue (Adcock, 2007)
La Hacienda Musa in Costa Rica was a long way from Leuven, Belgium. But for Maria Keller, the transition was as natural as it could be. She had spent 20 years in Leuven studying banana genetics at the Catholic University of Leuven’s Laboratory of Tropical Crops, the world center of banana research. She had learned about the challenges the banana-growing industry faced from a variety of diseases, why bananas seemed to be especially susceptible, and how difficult it is to develop new strains of the world’s most popular fruit. But after two decades of study, Maria was ready for
The DNA of organisms that is altered artificially, via a method known as gene splicing (Schmidt 2005, A.527), is referred to as Genetically Modified (GM). Presently, most existing GM foods originate from plants, however, in the future, food may be obtained from GM animals or micro-organisms (Bawa and Anilakumar 2013, p.1036). The subject of GM foods including crops, vegetables and fruit and how safe they are is the cause of many controversial debates globally, however there are several arguments that support the encouragement of genetically modified food production. Enthusiasts of GM foods maintain it will assist to end world hunger and encourage health and environmental wellbeing (Panse 2014, para.1). Further, the contention by GM enthusiasts is that crop yields are increased by the use of GM technology, the need for chemicals traditionally used in agriculture diminishes and is likely to result in the production of high-sustenance foods capable of growing in exhausted soils and drought-ridden locations (Schmidt 2005, a.527). Potential uses in the future include aiming modifications to improve nutrient content, decreasing allergen capabilities, and refining the productivity of food manufacture. As global water supplies diminish, many countries will no longer have the capabilities of producing stable crops, thus there is considerable significance placed on the advancement of biotechnology used to yield water efficient and drought-resistant food sources (MIT 2015, para.1).
La Hacienda Musa was a long way from Leuven, Belgium. But for Maria Keller, the transition was as natural as it could be. She had spent twenty years in Leuven studying banana genetics at the Catholic University of Leuven’s Laboratory of Tropical Crops, the world center of banana research. She had learned about the challenges the banana-growing industry faced from a variety of diseases, why bananas seemed to be especially susceptible, and how difficult it is to develop new strains of the world’s most popular fruit. But after two decades of study, Maria was ready for something new. She did her homework, packed her few possessions, and headed to her newly purchased banana plantation in Costa Rica. To say that La Hacienda
The breeding of the red-fleshed apples was originally from Kazakhstan and improvements of the apple was breed in order for the breeders to produce a apple that has high in Vitamin and as a healthy diet snack. The scientists have collected the apple’s seeds in order to let those seeds to grow in New Zealand. The red-fleshed apples were usually small back in Kazakhstan and it has a different taste which was a bit bitter and had a lack of quality in the commercial marketing. Also they have improved the apple’s taste just by crossing the original red-fleshed apple with another white-fleshed varieties fruit in order to improve the characteristics of the apple since it will be important for commercial and consumer with its long storage life. Generally, the red-fleshed apple will breed in order to improve the quality of the apple and also to provide nutrition which are high in vitamins and antioxidants, which then it reduces the risk of some diseases, but there are some red fleshed apple that can be healthier than other apples that have been produced. Although, breeders are able to use genetic information in order to make the breeding process faster and more effective. By investigating the DNA from the apples seed the scientists are able to predict that many apple characteristics including the red fleshed apple long before the seeds produces fruit, in order to decided which seeds are able to grow well and which can be redundant.
Due to the DNA’s specificity, samples can be utilised for identification. DNA is a nucleic acid composed of deoxyribose sugar bound to a phosphate group and one of four nitrogenous bases (adenine, guanine, cytosine and thymine). Each section of these three components are referred to as nucleotides, which are joined to the phosphate or sugar of another nucleotide by strong covalent bonds to form a backbone. The nitrogenous bases are joined to complimentary bases of another nucleotide (adenine with thymine, guanine with cytosine) to create a double stranded molecule (Figure 2). To complete the double helical structure, the molecule coils to compact it’s contents. DNA molecules can contain up to two million base pairs, with a human genome containing approximately 3 million base pairs. The random assortment of nitrogenous bases as well as the numerous mutations within certain DNA sequences, results in genetically diverese DNA molecules and genomes between individials.