DNA extraction in Watermelon and Bananas
Abstract During this experiment the researcher investigated the differing amounts of DNA present in bananas and watermelon. So that the researcher could do this, and accurately record the amounts, the researcher liquefied the fruits in a blender, added water and salt to them, and the added laundry detergent before using contact solution as an enzyme. Rubbing alcohol was then poured into the mixture and the DNA was extracted and put into a small container so that it could be weighed. This experiment gave the researcher the knowledge that bananas do in fact have more DNA than watermelon. The results were recorded in a chart and expressed through…show more content… 20. Put 6 ml of contact cleaning solution in each container. 21. Stir gently. 22. Tilting the container to the side, add a layer of rubbing alcohol. 24. Let the mixtures sit for 15-20 minutes, and watch the DNA rise to the surface. 25. Using the small strainer, separate the DNA from the rest of the solution. 26. Place the DNA in the plastic containers, the Banana DNA in one, and the Watermelon DNA in the other. 27. Weigh each on the gram scale. 28. Record the data.
|Fruit |Weight of fruit |Weight of DNA |Fruit to DNA ratio |
|Banana |114g +-1g |18g +-1g |19:3 |
|Watermelon |114g +-1g |3g +-1g |19:1 |
Conclusion While working on this experiment, the researcher was sure that the banana would have more DNA in it than the watermelon. In the end, this proved to be true with the banana getting 18 grams of DNA while the watermelon only got 3 grams of DNA, proving the hypothesis to be correct. The next time this experiment is attempted, the researcher would also like to test the difference of the amount of DNA in a bananas peel versus its