Influenza A Virus Lab Report

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Viral cell culturing has become a commonly used method for the cultivation of viruses. Before cell cultures, many viruses were propagated by the use of an embryonated egg. However, today embryonated eggs are still used for the production of vaccines and for the isolation of avian and influenza viruses. In addition, there are several procedures that must be carried out when a virus is grown in an embryonated egg. For instance, the eggs must be candled (to determine viability) and disinfected before a hole is drilled. Next, the virus must be injected into the appropriate area of a 5-14 day old embryonated egg. Signs of successful viral growth include pocks, hemagglutination, or death of the embryo 2-5 days after injection. In this lab, the purpose was to inject Influenza A Virus (H1N1) strain APR/8/34 into the chorioallantoic sac of a 9-11 day old embryonated egg. Our hypothesis was that the Influenza A virus will grow successfully in the embryonated egg to yield successful results for the following lab. …show more content…

Hemagglutination is defined as the aggregation of red blood cells in the presence of hemagglutinin virus particles (Woytek, 2017). Some of the benefits of using a hemagglutination assay includes its rapidity and ease of titration of a large number of samples. In this lab, the purpose of this experiment was to conduct a HA assay to obtain a titer for a virus stock and the Influenza A virus harvested. Our hypothesis was that hemagglutination of the Influenza A virus compared to the virus stock will produce a successful

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