Introduction . Enzymesare Proteins That Act As Catalysts
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Introduction
Enzymes are proteins that act as catalysts within living cells. Catalyst increase the rate at which chemical reactions occur without being consumed or permanently altered themselves. A chemical reaction is a process that converts one or more substances (known as reagents, reactants, or substrates) to another type of substance (the product). As a catalyst, an enzyme can facilitate the same chemical reaction over and over again.
Like all proteins, enzymes are composed of one or more long chains of interconnected amino acids. Each enzyme possesses a unique sequence of amino acids that causes it to fold into a characteristic shape. An enzyme 's amino acid sequence is determined by a specific gene in the cell 's nucleus. This…show more content… Them I pour extract into a conical centrifuge tube and centrifuge the extract for 5 for 2 minutes. Them I decant the supernatant into a clean test tube and then put the tube in ice. This extraction I will use as a source of peroxidase for experiments B, C,D and E
PartB
Effect of Enzyme Concentration on Activity
Tube ml Buffer (PBS) Ml OPD ml Hydrogen Peroxide ml Enzyme (Potato extract) Absorbency
1 (Blank) 3.3 0.1 0.1 0 0
2 3.3 0.1 0 0.1 .572
3 3.2 0.1 0.1 0.1 .885
4 3.1 0.1 0.1 0.2 .905
5 3.0 0.1 0.1 0.3 1.020
Material
Test tubes and test tube rack, spectrophotometer tubes or cuvettes, spectrophotometer, phosphate buffer solution ( pH7.0 ), hydrogen peroxide solution, OPD, vortexes, enzyme extract from potato, graduate pipette pump and micropipette and micropipette tips
Procedure
I number five test tubes 1-5 them I load all five test tubes with the buffer, hydrogen peroxide, and OPD in the order listed above. Them I add the enzyme to each test tube sequentially with a 2 minute interval because the moment I add the enzyme, the enzymatic reaction starts. Them I vortex all the tubes gently when all contents have been added, after each tube was incubate for 5 minutes at room temperature. Them I start to measure the absorbency ( A) at 410 mm of each tube #1 as a blank to calibrate the spectrophotometer them I start to record my results in the table above. To conclude the graph has a bell curve on an absorbency vs amount of enzyme added. This