The Effect Of Ph On The Function Of The Enzyme Amylase

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Discussion – Effect of pH on the Function of the Enzyme Amylase

The objective of the lab was to examine the effects of environmental variables on the functions of an enzyme. To the point, an experiment was conducted to test the effect of pH on the function of the enzyme Amylase. Enzymes are proteins that act as catalysts and help reactions take place. In short, enzymes reduce the energy needed for a reaction to take place, permitting a reaction to take place more easily. Some enzymes are shape specific and reduce the energy for certain reactions. Enzymes have unique folds of the amino acid chain which result in specifically shaped active sites (Frankova Fry 2013). When substrates fit in the active site of an enzyme, then it is able to catalyze the reaction. Enzyme activity is affected by the concentrations of the enzymes and substrate present (Worthington 2010). As the incidence of enzyme increases, the rate of reaction increases. Additionally, as the incidence of substrate increases so does the rate of reaction. Almost all enzymes need specific conditions for them to function. The conditions include temperature, pH level, and concentration of salt. Enzymes have optimal conditions. If they are changed, the enzyme may denature and deactivate. If that happens, the enzyme would not be able to catalyze the reaction, and the reaction rate would decrease (Worthington 2010). Amylase is a digestive enzyme. The enzyme is found in the salivary glands in your mouth

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