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Ion Exchange Chromatography Lab Report

Decent Essays
1. Ion exchange chromatography is a technique, which separates a mixture of amino acids based on their produced charge. Isoelectric point is a pH where the given amino acid has a no charge thus; produces a neutral structure. With a pH above the isoelectric point will cause the amino acid to be deprotonated and thus; produce a negative charge where as a pH lower than the isoelectric point will cause the amino acid to be protonated and thus; hold a positive charge.

2. In the experiment a cation exchange column was used to separate the mixture of amino acid. Cation exchange column is a negatively charged column where amino acids with positive ions would stick while, amino acids with negative ion would repel and elude out of the column. Three unknown buffers were used with pH of 3.5,6.5,and10.5 and the
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We then tested our predictions by performing TLC plate where three known amino acids were spotted against the three unknown buffers at the individual fraction numbers to produce a constant solvent migration distance of 3.06. Fraction number 5 migrated at the distance of 2.25 to produce Rf of .74 this closely resembles the Rf of Aspartic acid of .82. Fraction number of 9 migrated at the distance of ¬¬3.00 to produce Rf of ¬¬.98 this matches to the Rf of Phenylalanine .94. Fraction number of 8 migrated at the distance of ¬¬1.07 to produce Rf of .35 this matches to the Rf of Lysine .41 all three results match our predicted results.

5. Ion exchange chromatography has many uses from purifying proteins to analysis of pharmaceutical drugs, but one of the vital applications of ion chromatography is used in purifying water. Many individuals have been facing health problems after drinking water, which contains anions such as chloride and sulfate. These anions cause negative side effects in our body therefore; U.S. National Primary Drinking Water Standards are now using ion exchange chromatography as a tool to remove these harmful anions commonly found in impure
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