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Lab Frog Muscle (Guide Experiment)

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Lab 6 Biomechanics of Frog Skeletal Muscle

I. Purpose

This exercise is designed to demonstrate some mechanical and physiological properties of skeletal muscle using the gastrocnemius muscle of a frog.

II. Performance Objectives

At the end of this exercise the student should be able to:

1. Define minimal (threshold), subminimal, maximal, and supramaximal stimulus. 2. Explain what is meant by a “graded” response. 3. Draw a diagram of the setup used in this lab exercise. 4. Calculate the work that is done in a given muscle. 5. Describe the function of a force displacement transducer. 6. Describe what happens to muscle contraction when the load is increased. 7. Define twitch, treppe, tetanus and incomplete tetanus. 8. Explain …show more content…

This is the “muscle – bone preparation” you will use for your muscle physiology experiments. Connect the femur to the femur clamp as shown in VI. Steps IV, V and VI can be done concurrently.

IV. iWorx Frog Skeletal Muscle Physiology Setup. If the system is already set up, skip to V. Standardize the movement of the transducer

1. Make sure computer and iWorx interface (black box) are unplugged) 2. Attach the cable of the Displacement Transducer to the bottom channel 3 input plug 3. Insert the “stimulator cable” plug into channel 3 of the iWorx box (which is attached to the stimulator section of the iWorx interface) 4. Plug the computer power cord into the power outlet. 5. Plug the iWorx power cord into the outlet 6. Turn on the iWorx interface 7. Turn on the computer 8. After the computer has booted up, click on theLabscribe icon 9. On the Main window, pull down the Settings menu and select the Skeletal Muscle-Summation-Tetanus-LS2 settings file. 10. After a short time, LabScribe will appear on the computer screen as configured by the Skeletal Muscle-Summation-Tetanus-LS2 settings.

Your equipment is now ready for the experiment

V. Standardize the movement of the transducer

1. When the transducer rod is pulled by the contracting muscle, the amplitude of the contraction will be given as “volts” in the

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