Microscope are useful in viewing the letter “e” and the cheek cells. It shows a close visual image, which help to observe the two objects. Following the methods is important, because it provides the necessary materials and the steps of the procedure. It’s extremely important to wear a apron , goggles, and gloves. Also, be cautious in using the materials. The purpose is to observe and how to use the microscope under the specimen. For the results each figure image shows the differences between the low-power and the high-power objectives. In those figure, it will explain the view of the image of a cheek cells and a close image of how does the letter “e” looks like.
Introduction Anton Von Leeuwenhoek was the inventor of the
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Following the procedure.
Cut out the letter “e” and place it on the slide.
Add a drop of water using the dropper in the letter “e” on the slide.
Place the cover slip edge on the top of the slide and hold at a 45 degree angle. Avoid the bubbles. Than draw the image for figure 1.
Place the slide on the stage of the microscope and view in the low power (4x). Center the letter “e” in your field of the view. Draw the image for figure 2.
Move the slide to the left, to the right, up and down. See what happen.
Finally, view the letter “e” in high-power (40x). Use the fine adjustment to focus. Draw the image in figure 3.
How to prepare a cheek cells.
Use a small drops of methylene blue on the slide.
Using a toothpick, gently scrape the inside of the cheek.
Place the toothpick tip into the methylene blue and mix it.
Place the cover slip edge on the top of the slide and hold at a 45 degree angle. Avoid the bubbles.
Place the slide on the stage of the microscope and view in low-power (4x). Draw the image for figure 4.
Switch to high-power (10x). Draw the image for the figure 5.
Label the nucleus, the cell membrane, and the cytoplasm.
Results Looking though the microscope of the letter “e” in low-power objective with a total magnification 400x. In figure 2, the texture of the letter
Concept 6.1 Biologists use microscopes and the tools of biochemistry to study cells 1. The study of cells has been limited by their small size, and so they were not seen and described until 1665, when Robert Hooke first looked at dead cells from an oak tree. His contemporary, Anton van Leeuwenhoek, crafted lenses and with the improvements in optical aids, a new world was opened. Magnification and resolving power limit what can be seen. Explain the difference. Magnification is the ratio of an object’s image size to its real size. Resolution is a measure of the clarity of the image; it is the minimum distance two points can be separated and still be distinguished
Preparing specimen for electron microscope hard, light microscope still very useful as a window on living cells.
1) Apply the stain to your first unknown slide and examine it under the microscope.
The illuminating parts of a microscope enable us to see the detail of the subject placed under the microscope. The three main parts that enable us to do this are: the condenser which illuminates the object that is placed under the microscope, the objectives which forms the magnified image, and the eyepiece which enables us to see the magnified
Click the arrow in the bottom right corner of the screen to proceed to Slide 2 and begin the Click and
6 Place tray under enlarger. Stop down lens 2 stops. Make a step test on print (5-25 seconds).
As soon as the water was added a timer was set for two minutes and the slide was placed under the microscope.
In order to conduct this experiment you will need to know all the major steps. First, you will grab the depression slide and use your eyedropper to collect a single Daphnia and place it in the indentation of the depression slide. Make sure to remove excess water with the corner of your small paper towel. No coverslip will be necessary.
Since microorganisms are not visible to the eye, the essential tool in microbiology is the microscope. One of the first to use a microscope to observe microorganisms was Robert Hooke, the English biologist who observed algae and fungi in the 1660s. In the 1670s, “Anton van Leeuwenhoek, a Dutch merchant, constructed a number of simple microscopes and observed details of numerous forms of protozoa, fungi, and bacteria” (Introduction to Microscopes, n.d.). During the 1700s, microscopes were used to further explore on the microbial world, and by the late 1800s, the light microscope had been developed. “The electron microscope was developed in the 1940s, thus
They may have poor eye sight, the cells may not have transferred on to the slide properly, the microscope may have malfunctioned,
Many important scientists contributed to the development of the microscope, however, perhaps the most influential is Anton van Leeuwenhoek. Known as the father of microbiology, Van Leeuwenhoek was the first to discover bacteria through the use of microscopes that he developed himself (2, 3). Since then, many more scientists have made other changes leading to even more advancements in microscopy. Some of the microscopes most commonly used in microbiology include bright field, dark-field, phase-contrast, fluorescence, and differential interference contrast (DIC) microscopy (1). For this particular report, bright-field microscopes are used to visualize the unknown bacteria. Microorganisms are able to be viewed using the contrast of the microbe against the bright background of the slide (1). Sometimes it can be difficult to view microbes against a light background because they will seem transparent or have little contrast. To increase the contrast and allow for easier viewing, microbes are often stained. A condenser allows visible light to be focused and create a contrast between the background and the specimen. The microscope contains 4x, 10x, 40x, and 100x objective lenses. The 4x and 10x are used in order to scan the slide and determine the general location of the stained bacteria while the higher magnification lenses are
Procedure: First, set up the microscope. Clean the ocular lenses and objectives with lens paper. Then pace the prepared e slide on the stage and make adjustments. Turn the rotating nosepiece until the 10x objective is above the ring of light coming through the slide. Move the slide using the X and Y stage knobs until the specimen is within the view. Adjust the focus by looking into the eyepiece and focusing the specimen with the coarse then fine focus knobs. Adjust diaphragm until there is sufficient light
NOTE: Answer Question A only if you used a compound light microscope for this experiment.
Most microscopes, including those in schools and laboratories today, are optical microscopes. They use glass lenses to enlarge, or magnify, an image. An optical microscope cannot produce an image of an object smaller than the length of the light wave in use. To see anything smaller than 2,000 angstroms (about 1/250,000 of an inch) a wave of shorter length would