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Mast Cells Lab Report

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previous report, labelling of mast cells with fluorochrome labelled IgE did not induce activation or calcium signalling. Within the trachea, dendritic cells were predominantly localised closer to the luminal surface of the epithelium whereas mast cells were present throughout. Very few mast cell were found to interact with dendritic cells under homeostasis or post-antigen challenge. Live imaging highlighted the sessile nature of dendritic cells and mast cells in the absence or presence of antigen challenge, although the number of dendritic cells increased post-antigen challenge. Within the trachea, dendritic cells were found to extend dendritic projections under the airway epithelium but not into the airway lumen. Mast cells were found to…show more content…
This methodology was able to successfully label ~90% of Mcpt5-EYFP cells in the dermis and did not alter mast cell degranulation. Similar to the studies detailed above40,59, intravital imaging of mast cell degranulation highlighted the extravasation of vascular dyes. Interestingly, the vascular leakage occurred before any detectable release of labelled mast cell granules, probably mediated by histamine release after activation. Post-degranulation, few of the mast granules appeared to be transported further into the tissue, akin to a previous report60 where intact mast cell granules travelled to draining lymph nodes. Fluorescent avidin based labelling of mast cells in the IL-10 GFP reporter strain showed the presence of predominantly Av.SRho+GFP- mast cells under homeostasis. Post-challenge, mast cells were identified as a source of IL-10 within the activated tissue with more than 50% of avidin labelled mast cells expressing GFP24. However, within 2 days, other immune cells contributed to the production of IL-10 with mast cells only constituting approximately 10-15% of GFP+ cells within the inflamed tissue. The presence of mast cells and IL-10 was intimately linked to the suppression of inflammation during severe contact hypersensitivity. The release of mast cell-derived mediators either acutely via pre-stored granules or post-transcription has long been known to modulate innate and adaptive immune response2,3. The recent
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