Mixed Culture Report

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Introduction The purpose of this experiment was to allow each student to perform certain procedures and utilize the skills accumulated over the semester in order to interpret and identify two unknown organisms within a mixed culture. Each student was given a mixed culture containing one Gram negative organism and one Gram positive organism. I received unknown mixed culture #15. The possible Gram negative organisms within the mixed culture include: Enterobacter aerogenes, Pseudomonas aeruginosa, Neisseria perflava, Proteus vulgaris, and Moraxella catarrhalis. The possible Gram positive organisms within the mixed culture include: Corynebacterium xerosis, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, and Clostridium perfringens.
Using the mixed culture given, each student performed two Gram stains, which is described in the lab manual Microscopy And A Survey Of Microorganisms in Exercise 6 (McPherson pg. 53). During each procedure, every student followed aseptic technique in order to minimize contamination. This process is explained in Exercise 4 (pg. 37). Following the two Gram stains, each slide was viewed using a bright light microscope in order to find out the initial morphologies and different Gram
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66). The two TSA plates were incubated both aerobically and anaerobically because it displayed how oxygen affected the growth of each organism. Both aerobically and anaerobically incubated plates displayed identical white, convex elevated isolated colonies with entire margins and glossy, convex elevated isolated colonies with entire margins. Therefore, this procedure did not eliminate any of the possible organisms due to the fact that S. aureus, S. epidermis, E. faecalis, E. aerogenes, P. vulgaris, and P. aeruginosa all grow under aerobic and anaerobic
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