Confocal Microscopy of Cerebral Cortex Neurons damaged by Doughnutostupidiotis Introduction The Simpsons are constantly in the limelight with their own reality television show however, celebrity status does not protect Homer Simpson from a diagnosis of stupidity, caused by the over indulgence of icing coated, ring shaped boiled pieces of bread. According to the Butter and Jelly (2000) the overeating of this food item lead to Mr. Simpson falling into a coma for several weeks, before waking up. Doctor Riviera (2013) followed the case of Mr. Simpson through his diagnosis of doughnutostupidiotis through to his inevitable death. According to Riviera (2013) the key symptoms of this disease is reduced executive functioning in the frontal lobe which …show more content…
The aim of this experiment is to examine Mr. Simpson’s brain and investigate if there is any structural changes at the cellular level that would explain Simpson’s condition and perhaps answer why there was a significant amount of gene coding seemingly removed from his cells. Rivera hypothesised that perhaps a mutant virus that was passed on by excessive consumption of doughnuts had eaten through cells in the cerebral cortex leaving behind hindered gene coding that was effective enough for Homer to function at a baseline level. To investigate this the following techniques of confocal microscopy and fluorescence labelling were …show more content…
The Thermofisher website (2015) was used to determine which labels would best suitable for this experiment. For imaging the cell nuclei the marker HCS NuclearMask Blue Stain will be used as it can be utilised to measure DNA content. In terms of this experiment the whole cell was stained, this marker labelled the entire cell including the cytoplasm and nucleus. This particular marker is excited by Ultra Violet light at 355nm and required an emission filer for 350/451nm. The second labelled used in this experiment was Alexa Fluor-647 that was used to fluoresce the axonal projections for cells. This marker is excited by a light source which in this experiment was a laser at 640nm and required an emission filter for the floid red channel which covered 646/68nm. These markers were already prepared and just needed to be added to the cell slides and incubated over night to ensure a good enough
The undersigned detective interviewed Ms. Terry Jarrett (Psychiatrist). Ms. Jarrett stated that Mr. Simpson has been a patient at the clinic since 9/03/2014. Ms. Jarrett stated that Mr. Simpson's suffering from intellectual deficiency, and he thinks much younger than his appearance. Mr. Simpson has no one caring for him at home and play with children in the neighborhood. Ms. Jarrett stated that Mr. Simpson is psychotic and "very non compliance" to his treatment.
It is a great pleasure to recommend Jenny Simpson for admission to your Master of Arts in Curriculum and Instruction program. During the previous academic school year, I had the privilege of coaching and managing Jenny. Given that there is not an algorithm for determining excellence in teaching, I would say that she consistently demonstrated certain characteristics and actions that would set her apart from other teachers in our program.
Tim Morgan hit Michael Kramer with a foam hand after a verbal argument at a Patriots/Steelers professional football game in Foxborough, MA. The actions of Mr. Morgan caused Mr. Kramer to fall down the bleachers and sustain injuries. Mr. Morgan is facing charges of assault and battery with a dangerous weapon.
This paper conducts research on Orenthal James ‘O.J.’ Simpson’s 1994 murder trial of his ex-wife Nicole Simpson-Brown and her friend Ronald Goldman, in which Simpson was acquitted.This trial is one of the most televised court cases in history. Once a well-rounded guy, Simpson born in San Francisco, had a successful college football career at USC, winning the Heisman Trophy. Simpson then went onto the NFL playing for the Buffalo Bills as a star running back. The O.J. Simpson trial was majorly sensationalized due to the fact that he was one of the first african-american sports figure who 's crime was televised through all channels of media. I am going to discuss how he was considered not guilty in how is defense lawyers were able to get the charges acquitted. In this case money certainly bought Simpson justice but in a few other cases money did not buy people with celebrity status justice.
The role of trial consultants in each particular case differs. These roles can vary from solely assisting with jury selection to a full range of services including conducting community attitude surveys, determining the effectiveness of evidence that will be used in trial, guiding attorneys through what approach should be taken during the trial, and preparing witnesses to testify in court. The different roles are an integral part of trial consulting as defined by the field of psychology. Field consultants are said to provide insight and guidance on the case through the roles described above. To determine if these roles described are that of the real world this paper will be discussing one case in particular that highlights the differing uses for trial consultants, the O.J. Simpson trial. Simpson was a former professional football player, idolized and known by many, who was tried for the death of his ex-wife, Nicole Brown, and a family friend, Ronald Goldman (Ford & Newton, 1994). Many people know this trial because of the high level of media attention it received but what is unknown to various people is the work that was put in by trial consultants Jo-Ellan Dimitrius and Don Vinson. During this trial, the consultants focused mainly on assisting with jury selection, conducting community attitude surveys, and guidance on trial strategies. The trial consultants had to find a way to work around
The O.J. Simpson Trial will always be one of the most discussed cases in history. The well-known former football-player-turned-sports-announcer married Nicole Brown in 1985. Around ten o’clock on the Sunday night of June 12, 1994, a single male came through the back entrance of Nicole Simpson’s condominium located on Bundy Drive. The man brutally slashed Nicole’s neck, nearly severing it from her body, then stabbed Ronald Goldman, Nicole’s current boyfriend at the time, at least thirty times. Whether Simpson is guilty or innocent is not up for debate, but I am here to present a few facts.
Many Americans can still remember where they were when O.J. Simpson’s murder verdict was broadcast on national television in 1995. O.J., a former football and movie star, was on trial for the brutal murder of Nicole Brown Simpson and Ronald Goldman on the night of June 12, 1994 in Brentwood, California. Alan Dershowitz, a member of Simpson’s dream team of lawyers, published in his book America on Trial, that during the verdict, long-distance telephone call volume declined by 58% and trading volume on the New York Stock Exchange by 41%, and water usage decreased as people avoided using bathrooms (13). The real-life trial was like a television drama series, and people stopped their daily activities to watch it unfold. People from all over gathered in homes and public places to witness the conclusion on live television. So much work stopped that the verdict cost an estimated $480 million in lost productivity (Dershowitz 13). When the verdict was delivered and O.J. was found not guilty of murder, the reaction of the American people was varied. Some people danced with joy, others cried in sorrow, and some just watched in shock. Unfortunately, the social issues of the region and the manipulation of those issues seemed to sway the jury away from the presented facts of the case. The evidence provided during the murder trial proved that O.J. Simpson was indeed guilty of stabbing and killing Nicole Brown Simpson and Ronald Goldman on the night of
Twenty two years ago, football star, O.J. Simpson, was put on trial for the murders of his ex-wife Nicole Brown Simpson and Ron Goldman. Despite the fact that he was found not guilty, some still believe he is guilty of these murders. The evidence from the trial, however, proves that O.J. Simpson is innocent and did not kill Nicole Brown Simpson and Ron Goldman. O.J. Simpson was born in 1947 in San Francisco, California. "He became a college football star at USC, winning the Heisman Trophy in 1968, and later enjoyed a record-setting career in the NFL".
TRAVERSE CITY, Mich. –– Homer Simpson has announced the challenging triathlon, Iron Cross, to be scheduled on June 6, at Traverse City City Hall.
During the interview Tillman Freeman III demanded information from his wife pertaining to her have multiple affairs. It appeared to me he wanted to make her suffer emotionally. Tillman Freeman III continued to ask Candice Freeman about who she was sleeping with. During the interview he asked Detective J. Figueroa to call an individual suspected of sleeping with his wife. During the phone call Tillman Freeman III told the male Serenity Freeman was his child. After the phone call was completed Tillman Freeman continued to accuse Candice Freeman of having an affair. After Candice Freeman left from the interview room, Detective J. Figueroa continued to talk with Tillman Freeman III. During the conversation Tillman Freeman III agreed to take Detective J. Figueroa to where the children were, which was somewhere in Hoke County.
I would like to make a few comments about Major Simpson. Actually, Erika threatened me with bodily violence if I didn’t say a few words. To start, I have these phrases in my officer: The Burdens of leadership are great. One of them is to be unpopular when necessary. The other phrase reads like this: What is popular is not always right; what is right is not always popular.
diH20 acted as a negative control in the experiment, all cells, both in intrafollicular zone and in interfollicular zone stained purple. Cells stained brown in the negative control indicate non-specific staining.
Plants are organisms that can reproduce sexually through meiosis and create more cells through mitosis (Russell et al. 2013). For studying mitosis, the common onion is an ideal choice. Because it is easy for onions to germinate without soil, it is easy to control any substances provided to the plant. The onion root tips are only a few cells thick and grow quickly making them ideal for time efficiency. The onion root tip needs to be squashed between the cover slip and the microscope in order to reduce the slide preparation’s total depth. To dye condensed chromosomes, such as those undergoing mitosis, a stain is used to make
Making the onion tip root cell slide was successful. Our results supported the hypothesis because we saw cells in the onion root tip in prophase, metaphase, and anaphase. As we went up in power objectives, each phase of the cell became more definitive. The cell root was a great indicator of the structures of the different cycles of the cell. This is important because we will be prepared for future labs working with the microscopes and can now adjust it for the best view of the slide. We practiced working with the compound light microscopes and different phases of the cell cycle. Onion root tips are useful to observe mitosis because the cells are frequently diving as the root grows. So when we stained the cell, we caught many cells in different phases. The significance of this lab was to better understand the process and stages of mitosis and meiosis and compare and contrast the mitotic process in plants and animals. We grasped the concepts of what the chromosomes look like, and what they look like in each step of the processes. Having read much about mitosis and meiosis, seeing these cells was the real application of describing and understanding the stages.
Summary: The basic idea behind cell tracing, as the term suggests, is observing a cell or population of cells over time to record the changes occurring within them. Prospective lineage analysis allows the tracing of pre-selected candidate cells to study their outcome. Whereas, retrospective lineage analysis refers to the systematic analysis of a population of cells at the end of a study/sample collection to determine their origin(s). The simplest method of cell tracing is by direct observation of the cells under the microscope without incorporation of an artificial label, and is commonly applied to study development in lower organisms. When direct observation is not feasible, the introduction of an exogenous marker within the cells facilitates their tracing. There are several ways to introduce an exogenous marker — either by genetic manipulations or by non-genetic means (such as by using vital dyes). Genetic manipulation is preferred to label cells, especially in long-term experiments, as they allow stable incorporation of the label into the cells that remains undiluted over time. Additionally, using genetic methods permits the expression of the label to be constitutive, cell-type specific, or inducible, thus providing greater flexibility to the researcher and allowing both spatial and temporal control over labeling. Cell tracing using genetically introduced labels is called “genetic tracing”. In the following section, I will elaborate on the ROSA genetic tracing approach, a