Making the onion tip root cell slide was successful. Our results supported the hypothesis because we saw cells in the onion root tip in prophase, metaphase, and anaphase. As we went up in power objectives, each phase of the cell became more definitive. The cell root was a great indicator of the structures of the different cycles of the cell. This is important because we will be prepared for future labs working with the microscopes and can now adjust it for the best view of the slide. We practiced working with the compound light microscopes and different phases of the cell cycle. Onion root tips are useful to observe mitosis because the cells are frequently diving as the root grows. So when we stained the cell, we caught many cells in different phases. The significance of this lab was to better understand the process and stages of mitosis and meiosis and compare and contrast the mitotic process in plants and animals. We grasped the concepts of what the chromosomes look like, and what they look like in each step of the processes. Having read much about mitosis and meiosis, seeing these cells was the real application of describing and understanding the stages.
Questions:
MITOSIS IN ONION ROOT TIP SQUASHES (pg.44)
7. Examine under low power (100X) and then under high power (430X) . a. Can you locate the various stages of mitosis? Yes, we could locate the various stages of mitosis. The prophase, metaphase, anaphase, and telophase stages were clearly present in the cells.
The leptotene. This phase differs only slightly from the early stages of mitosis. Usually are the cells and nuclei of meiotic tissues bigger than that of their neighbouring tissues and often do they seem to be longer and are longitudinally structured. At regular intervals can thickenings be found, like beads on a string: the chromomeres. Their number, size and positioning is constant in each species.
In this experiment first the stages of an onion cell undergoing mitosis are going to be observed and every stage is going to be detected and drawn on paper. A brief description to what is going on should be attached to the pictures. This is important to understand the basics of cell division which is necessary growth,repair and asexual reproduction. Second the number of cells undergoing each phase is going to be counted to figure out in which phase the cell remains the most. If interphase is the stage in which the cell grows and prepares for cell division then the
According to the data table, prophase is the longest stage of mitosis. Even though there were more tallies in the interphase section, interphase is not considered a stage of the mitotic cycle; it is more or less considered a stage in the cell cycle. Telophase is the shortest stage because there were very few cells that showed evidence of them actually being in this stage. Metaphase and anaphase are intermediate stages, with metaphase being longer than anaphase.
Compare and Contrast Mitosis and Meiosis Meiosis and mitosis describes the process by which cells divide, either by asexual or sexual reproduction to produce a new organism. Meiosis is a form of cell division that produces gametes in humans these are egg cells and sperms, each with reduced or halved number of chromosomes. The number of chromosomes is restored when two gametes fuse together to form a zygote. A cell with two copies of each chromosome is called diploid cell and a cell with one copy of each chromosome is called a haploid cell.
Mitosis and meiosis are similar in several ways and different in others. The similarities include that both processes involve IPMAT. IPMAT is interphase, prophase, metaphase, anaphase and telophase. The parent cells are diploid. They both end with cytokinesis. In Metaphase and Metaphase II, the sister chromatids line up along the center. Then in Anaphase and Anaphase II, these chromatids are split and pulled towards the centrosomes. The differences are that mitosis consists of 1 division while meiosis consists of 2. Four genetically different, haploid sex cells are the products of meiosis and 2 identical, diploid somatic cells from mitosis. Mitosis occurs in all organisms except viruses and meiosis only occurs in plants, animals, and fungi.
“All types of cells undergo mitosis during formation of the embryo, but many adult cells, such as nerve cells, lens cells of the eye, and muscle cells, lost their ability to replicate and divide. The cells of other tissues, particularly epithelial cells [e.g., of the intestine, lung, skin), divide continuously and rapidly, competing the entire cell cycle in less than 10 hours” (McChance & Huether, pg. 35).
Although mitosis is just a fraction of time compared to interphase it can be divided into four distinct subphases. If we start viewing a cell under a brightfield microscope right at the end of interphase subphase G2 we would see that throughout the interphase process (fig 1) the cell has grown considerably in size due to production of extra mitochondria and endoplasmic reticulum. The cell has also gone through the labor of duplicating all of its chromosomes, a process known as DNA synthesis. Now that the cell has made all of these preparations it's time to start prophase (fig 2), the first stage of the mitotic phase. This stage is visibly identified by the chromatin becoming the tightly formed chromosomes. During the lab we were able to view these newly formed chromosomes best on the onion root tip
Find a representative cell for each stage of mitosis on the onion slide and draw a clear diagram of the different phases you observed in the accompanying space. Be sure to draw only what you see, but include all details that are visible. Your drawings will not necessarily look exactly like the ones in Figure 3A, 3B, 3C.
19) The phase of mitosis during which the nuclear envelope fragments and the nucleoli disappear is called
Mitosis is the fourth stage of the cell cycle. It includes two phases mitosis and cytokinesis. Mitosis is the division of the cell's nucleus and all it’s contents. Cytokinesis is the process that divides the cell’s cytoplasm. The stages of the cell cycle and the proteins are all alike in all
cell will look just like any other 'normal' cell although this is far from the
Mitosis is one of two main methods of cell replication, the other being meiosis. It is “the simple duplication of a cell and all of its parts” resulting from the splitting of a cell. The ‘parent’ or original cell splits, duplicating its DNA (packaged in chromosomes) producing two ‘daughter’ or new cells with the same genetic code. There are four stages of mitosis; Prophase, Metaphase, Anaphase and Telophase. Interphase is not an official phase of mitosis as the cell is at ‘rest’ and not being divided during this phase. It is often called one of the phases of mitosis as it is one of the stages in the life cycle of a cell; however it is not a phase of mitosis due to the fact that no division takes place.
Plants are organisms that can reproduce sexually through meiosis and create more cells through mitosis (Russell et al. 2013). For studying mitosis, the common onion is an ideal choice. Because it is easy for onions to germinate without soil, it is easy to control any substances provided to the plant. The onion root tips are only a few cells thick and grow quickly making them ideal for time efficiency. The onion root tip needs to be squashed between the cover slip and the microscope in order to reduce the slide preparation’s total depth. To dye condensed chromosomes, such as those undergoing mitosis, a stain is used to make
Cell division in plants is a process that is currently under intense study by biologists. One team of researchers has recently published a paper describing their research into the role that microtubules play in cell division and morphogenesis. While these structures have been identified to be involved in cell division and growth, how they form, disassemble, and reappear is not fully understood. The team's research consisted of their testing the hypothesis that a gene in the plant A. thaliana, called mor 1, plays a role in the development of microtubules.