My Life As A Microbe Project

Please answer these questions then place them in the drop box for this lab. Use Microsoft word if possible.

There are three types of environments in which cells are located which include isotonic, hypotonic and hypertonic. In an isotonic environment, the amount of water and solute are the same both inside and outside of the cell. As water drifts into the a cell, the same amount flows out creating a balanced environment both inside and outside of the cell. When there is a high level of water on the outside of the cell and a high amount of solute inside the cell, water will be drawn inside of the cell creating a hypotonic solution. The increase in water inside the cell causes the cell to become engorged and erupt. In a

1. Describe the function of the following pieces of safety equipment and how each might be used: (10 points)

|EMB Agar | |Distinguishes bacteria that ferment |Dark blue colonies with|E. coli and P. |

The experimental part of the lab consists of setting up the materials needed. A sample of E.coli and a solution of calcium chloride are first obtained and placed in different test tubes. 630µL of Calcium Chloride (CaCl2) are then removed from the test tube and inserted into the test tube containing E.coli cells (Alberte et al., 2012). The newly formed substance of Calcium Chloride and E.coli is then mixed and incubated in ice for 10 minutes, making the cells more competent. Two test tubes are obtained and labeled; the first test tube is labeled with pUC18 and the second one with “Lux” to represent the plasmids being used. These two test tubes are then incubated in ice. 3µl of the set plasmid are added to each of the two test tubes. The test tubes are tapped to guarantee the cells are well

Microorganisms are both beneficial and harmful. These microorganisms are important to humans because they play a role in the ecology of life, by decomposing wastes, both natural and man-made, such as creating nitrogen fertilizer at the root zones of certain crops. Other several pathogens that can cause serious harm, even immediate death due to the diseases or disease causing products they produce. Overall, microorganisms play an important role in life.

BSP, S. (2010). How is EM different from light microscopy? Retrieved April 25, 2015, from http://bsp.med.harvard.edu/node/222

The purpose of the “chi-square test” was to see if our data was in an acceptable range of a specific ratio listed above. The chi-square test took into account the expected deviations in the F2 offspring’s alleles.

The purpose of the bacterial unknown independent study experiment completed throughout the course of this lab was to determine the identity of an unknown bacterial species. The unknown bacteria sample was chosen from numerous samples provided by the instructor. The starting unknown sample, unknown #15 was a mixed bacterial culture and a broad approach taken to identify the sample. Various biochemical tests were completed to identify the bacterial species along with the use of databases such as Gideon and Bergey’s to compare the test results of known bacteria to the results of the unknown sample. Information was gathered from the other sources and databases and phenotypic testing completed and the results compared to the database results. Aseptic

In 1998, Biopure Corporation is one of the three legitimate contenders in the emerging field of “blood substitutes”. Biopure has invested $200M on the R&D on blood substitutes in the past with its primary goal being the development of a human blood substitute ( Hemopure) but Its entry into animal market (Oxyglobin) had been some

Prokaryotic Cells All living things are made of cells, and cells are the smallest units that can be alive. Life on Earth is classified into five kingdoms, and they each have their own characteristic kind of cell. However the biggest division is between the cells of the prokaryote kingdom (monera, the bacteria) and those of the other four kingdoms (animals, plants, fungi and protoctista), which are all eukaryotic cells. Prokaryotic cells are smaller and simpler than eukaryotic cells, and do not have a nucleus. Prokaryotic means 'pre-nucleus' and eukaryotic means 'true nucleus'.

The key issue is to determine when Oxyglobin should be introduced to the market without jeopardizing Hemopure’s potential and how it should be marketed.

In 1998, Biopure Corporation is one of the three legitimate contenders in the emerging field of “blood substitutes” along with Baxter International and Northfield

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

The law proves that natural selection is necessary for evolution to occur ("SparkNotes: population genetics," 2014). The conditions set up by the Hardy-Weinberg Law allow for variability (the existence of different alleles) and inheritance, but they eliminate natural selection ("SparkNotes: population genetics," 2014). The fact that no evolution occurs in a population meeting these conditions proves that evolution can only occur through natural selection ("SparkNotes: population genetics," 2014). The Hardy-Weinberg Law allows us to estimate the effect of selection pressures