Nutrient Agar Lab Report

Research Question: How does the size of the cell affect its efficiency in exchanging substances through several ways, like diffusion?

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

In the beginning of lab, we were advised to obtain a nutrient agar petri plate, which is used for the cultivation of microbes supporting growth of non-fastidious organisms. Since it contains many nutrients, a wide variety of bacteria and fungi can grow. Taking the plate,

In the LB (pGLO negative), it is expected to not see any colonies growing. As a result of this experiment, it shown any growth colonies but this only had shown a large number of white, circular colonies that were found across the surface of the agar. In the LB/Amp (pGLO negative), it is expected to see any growth colonies. In the experiment, the resulted was no growth colonies because this has Ampicillin and no pGLO. Now, the LB/Amp (pGLO positive), it is expected to have growth colonies in the agar plate. As a result, it was shown growth colonies in the agar plate because has positive pGLO regardless having Ampicillin. In the other hand, both positive pGLO have the same components but in one plate was added Arabinose. The LB/Amp/Ara (pGLO

Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.

When looking over my results, I found that the pH level increased from a neutral pH to a basic level of pH. The Miracle- Gro fertilizer that I used is supposed to have a pH of 7. This surprised me because I would think that adding a 10% fertilizer solution would make the water acidic. I thought that the fertilizer would be more acidic because when we learned about rivers, we found out that acid rain decreased the dissolved oxygen levels; also, Eutriporication results in low dissolved oxygen levels. Therefore I believed that the fertilizer would be acidic because of my background knowledge of dissolved oxygen in water. Regardless, the basic water killed the algae, elodea plant and the snails in the water. I also thought that eutrophication would occur in the water when

The identification of unknown organisms carries important ramifications that can be applied to many real world scenarios. In keeping with quality assurance beverages, food, cosmetics, and other products are frequently inspected for contaminants resulting from a presence of pathogenic bacteria. In medicine, a physician’s diagnosis and consequent treatment is largely determined from samples collected from infection sites that have been analyzed using microbial tests.

2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.

The investigation is showing how enzymes work inside a mammal's stomach. Rennin is the enzyme found in young mammals and has more effect

In typical neutral soil, the numbers of bacteria dominate over the number of fungi. Because of this, general growth plates often see little to no fungal growth when placed in these conditions. In order to select for fungal growth, an acidic medium is normally used because fungi grow readily at higher acidity (S.A. Waksman, submitted for publication 1922). This information is useful in determining the type of medium required to select for fungal growth.

After 48 hours, the experimenter was able to obtain the Petri dish in order to observe what had taken place, within the nutrient agar plate, of the span of this period of time. The experimenter was able to establish that any clear areas surrounding the filter-paper disks were the zones of inhibition and they indicated that the antibiotic was able to inhibit the bacterial growth, and any cloudy areas of the agar demonstrated that bacterial growth was still able to take place within the given conditions. Thus, the larger the zone of inhibition, the more bacteria was inhibited within this specific environment. Quadrant 1 contained an Erythromycin filter-paper disk, which was able to inhibit the growth of Bacteria D. The Erythromycin filter-paper

Label three test tubes A - C and place them in a test tube rack. Using a graduated beral-type pipet and add about 2 mL of the cobalt chloride solution to each test tube A - C. Make sure a pipet is used to measure so that all volumes are equal. Place A in an ice bath, B in boiling water, and C should remain a constant temperature. Add HCl to each and observe.

Studies show that unwashed or lightly rinsed vegetables can harbor pathogenic bacteria and have been implicated in numerous foodborne infections. The objective of this experiment was to obtain a quantitative viable plate count of enteric bacteria present in a sample of broccoli sprout water. To enumerate the number of bacterial colonies present in the sample, we performed serial dilutions of broccoli water spread onto MacConkey Agar plates. MacConkey Agar is a selective and differential media. It is used to select enteric bacteria and differentiate lactose-fermenters from non-lactose fermenters. Utilizing this process illustrated the potential amount of foodborne illness causing bacteria present on foods, specifically fresh broccoli

Each test performed in the lab on theses unknown bacteria have a very specific significance. With each test performed correctly the lab officer is able to move closer towards a proper identification of the unknown bacteria. After performing Gram staining and deciphering if the unknown was gram positive or negative the lab officer was then able to proceed to the next step of identification. The gram positive unknown’s reaction to the catalase test informs the tester of the Genus theyre working with. This indicates which tests to perform next. The MacConkey agar is a selective medium that only allows the growth of gram positive bacteria confirming the results received from the gram staining procedure. The NaCl growth test indicates whether or not the organism is able to

Carbohydrates are the product that made up from carbon, hydrogen and oxygen. Carbohydrates are form by the combination of carbon dioxide and water molecules. The carbohydrates contain two specific functional group in it which is the hydroxyl groups and carbonyl groups.A reducing sugar is a type of sugar with is an aldehyde group.This means that sugar can act as a reducing agent.The procces of reducing sugar is isomerisation,example of reducing sugar islactose,maltose,glucose and fructose.All monosaccharides are capable of reducing other chemicals such as copper (II) sulphate to copper oxide.Beside that disaccharides such as maltose and lactose are reducing sugar,however sucrose is non reducing