Prevalence Of M. Tuberculosis Infection

703 WordsJan 17, 20173 Pages
AIM OF THE STUDY: The aim of this study was to estimate the prevalence of M. tuberculosis infection in adult SLE patients & its relation to disease duration, activity, damage and treatment. Also, to compare the performance of IGRA in detection of M. tuberculosis infection with TST in those patients. PATIENTS AND METHODS: I- Patients: A cross-sectional study included 100 SLE patients fulfilling the American college of Rheumatology (ACR) revised classification criteria for SLE(8). All patients were recruited randomly from the Rheumatology outpatient clinic and the inpatient ward of Internal Medicine and Rheumatology department at Ain Shams University hospital. The nature of the present study was explained to all participants. The laboratory…show more content…
The skin test reaction was read between 48 and 72 hours after administration. The reaction was measured in millimeters of the induration (palpable, raised, hardened area or swelling). The diameter of the indurated area was measured across the forearm (perpendicular to the long axis). An induration of 5 or more millimeters was considered positive (15). Interferon Gamma Release Assays (IGRA) using The QuantiFERON-TB GOLD In-Tube (QFT-GIT) test: QuantiFERON® TB Gold In-Tube (Cellestis Limited, Victoria, Australia) was used according to the manufacturer’s instructions. QFT-GIT was considered positive if the IFN-γ level of nil was ≤ 8.0 IU/ml and that of TB antigen minus nil was ≥0.35 IU/ml and ≥25 % of nil value. The test was considered negative if the IFN-γ level of nil was ≤ 8.0 IU/ml and that of TB antigen minus nil was ≤ 0.35 and < 25% of Nil value. The result was considered indeterminate if the IFN-γ level of nil was ≥ 8.0 IU/ml(16). Microscopic examination of appropriate specimen for the presence of acid-fast-bacilli (AFB) and culture over Lowenstein Jensen (LJ) medium for tubercle bacilli were done for all patients with positive results of QFT-GIT or TST tests. Also, Plain chest radiography (posteroanterior view) was done; cavitary lung lesions, nodules, reticulonodular infiltrates, effusion and hilar lymphadenopathy were considered as CXR abnormalities (17). Latent
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