Recurrent incidence of babesiosis among apparently healthy bovines in Egypt, submits the needs for suitably destined prevalence studies with rapid and accurate diagnosis and immediate treatment of infected animals are required to control this disease. The routine epidemiological survey and diagnosis usually based upon microscopical examination of Giemsa stained blood smears. This technique is insufficient for accurate detection and identification of Babesia species during mixed infections and not reliable for detecting the infection in carrier cases or subclinical infections with low parasitemia. Combination of molecular and serological diagnostic tools for the infection provides powerful tools for accurate diagnosis as well as for epidemiological investigations as means of overcoming limitations of microscopical examination. The polymerase chain reaction will be conducted using highly conserved and species specific genes for detecting the parasite DNA in the blood of infected animals based on the B. bigemina rhoptry associated proteins, Apical membrane protein 1 & Internal trancribed spacer and B. bovis Spherical body proteins 4. The serological diagnostic tools, including the indirect fluorescent antibody test, the Indirect enzyme-linked immunosorbent assay, a competitive ELISA will be conducted to detect Babesia antibodies in serum of naturally infected cattle based on Babesia lysate, Cyanogen bromide-activated Sepharose 4B, affinity purified antigen and expressed,
In this experiment, both Coproantigen ELISA and Serology indirect ELISA were carried out on samples from a farmer which suspected his herds are suffering from Fasciola hepatica infection. The aim of this experiment is to find out if the samples are positive for Fasciola hepatica infection and discuss the difference between two tests.
Bronchiolitis is a lower respiratory infection caused commonly by the respiratory syncytial virus (RSV) in the first two years of life. Nearly every child in the United States will be infected with RSV before its second birthday. The condition is a leading cause of hospital admissions among children under the age of two. The populations most frequently and severely affected by bronchiolitis include; infants less than 12 months of age, children born at a low gestational age (less than 32 weeks), and children with chronic lung disease, congenital heart disease, or immunodeficiency. Standardized and proper diagnosis and management are essential to effective treatment. The guideline established by the Academy of Pediatrics (AAP) in 2014 provides practitioners with recommendations for diagnosis, management, and prevention of bronchiolitis in children.
In 1943 the Economic Administration Main Office made it the first civilian camp and then it eventually became a concentration camp.
A health-issue known to be documented in the pre-school setting is pertussis, or better known as whooping cough (Kid Source Online, n.d.). Whooping cough, as it’s name implies, is a very communicable, infectious disease of the respiratory tract, which causes the child to make a “whooping” sound when trying to draw breath after a prolonged cough spell (Kid Source Online, n.d.). Nonetheless, not all children are known to make this said “whooping” sound while having this disease; thus, one must be aware of the general symptoms indicating whooping cough, which include, however aren't limited to, a runny nose and an extreme cough, both which gradually seem to only get worse, and perhaps can even end with the child profusely vomiting (Kid Source Online, n.d.).
Hemorrhagic Disease of White-Tailed Deer is a phrase that describes an infection of either Epizootic Hemorrhagic Disease (EHD) and/or Blue Tongue Disease (BT). These two diseases are clinically and molecularly very similar. Since the symptoms of both diseases are indistinguishable molecular testing is needed to specifically identify these viruses. Both viruses are spread by biting midges in the Culcoides genus which is found in a majority of the US. Historically, there have been large outbreaks in the US and all over the world which has lead to economic losses of various degrees. With current expansion of these diseases vector, a lack of monitoring, and gap of knowledge about these diseases it is only a matter of time before a catastrophic
Whooping cough (pertussis) has been on the rise in the United States in recent years. Last year the CDC reported 28,660 cases of whooping cough in the U.S. alone. With cases increasing annually, protecting infants is imperative in controlling the disease. Understanding the signs and symptoms of whooping cough along with the recommended protocol for immunization can mean the difference between life and death for children in your home or care.
African horse sickness virus can be identified by antigen detection and nucleic acid detection (CFSPH, 2006; OIE, 2009). The whole virus can be detected from the sample using different identification techniques. Viral isolation, in outbreaks occurring outside the endemic areas, is of importance (CFSPH, 2006; Koekemoer, 2008). A variety of cell cultures can be used for the isolation of AHSV and these include BHK21 and Vero cells, (CFSPH, 2006; OIE, 2009). Other techniques that can be used to isolate AHSV include intracerebral inoculation of baby mice and intravascular inoculation of chicken embryo (CFSPH, 2006). Virus isolation through intracerebral inoculation of 2 to 3-day old mice is the preferred technique (Mellor and Hamblin, 2004). AHSV can be grown in insect cells such as mosquito cells (C6/36) and Culicoides spp (KC cells), but these do not show any cytopathic effect (Mellor and Hamblin, 2004). Group-specific tests such as complement fixation test (CFT), agar gel immune-diffusion (AGID), virus neutralization (VN), indirect fluorescent antibody (IFA) direct ELISA and or ELISA can be used for serodiagnosis of AHSV (House et al., 1990). The five serological tests are used to detect antibodies against AHSV (House et al., 1990). Williams et al (1993) developed a group-reactive ELISA tests that detect antibodies against AHSV and equine encephalosis virus (EEV) which are known not to cross-react. Maree and Paweska (2005) prepared recombinant AHSV’s VP7 antigen and validated a VP7-based indirect ELISA (I-ELISA) for the detection of group-specific IgG antibodies in horse
Borrelia burgodoferi is transmitted to humans and mammals by the Ixodes scapularis known as the deer tick or blacklegged tick and the Ixodes pacificus known as the
Whooping cough is a highly infectious disease that is spread by coughing and sneezing. It’s caused by bacteria which damage the breathing tubes.This pathogen produces a toxins that disrupt the normal function of cilia in the lungs, resulting in a critical cough.Whooping cough is caused by a bacteria (pertussis) it is contagious respiratory tract infection.It can be serious for anyone, but life-threatening for newborns and infants.
In cattle there are some common diseases. They are clostridial Diseases, Respitory Disease stress, and Parasites. The first is Colstridial disease and causes sudden death in young cattle. The diseases are Blackleg and Enterotemia. To prevent theses vaccinations should be given early in life and given boosters when needed. Respiratory disease stress can be form weather changes and infections. To minimize stress, the use of wind protection such as a shed or a windbreak. The best
A pathogen also known as an infectious agent, is an organism which causes illness or disease to its host. There are many different kinds of pathogens and the main ones are bacteria, fungi and virus. In this report I will be mentioning two pathogens which are caused by a particular bacteria, Mycobacterium Tuberculosis (MTB) and Whooping Cough. I will be explaining TB in detail and whooping cough briefly.
Buerger’s disease is an inflammatory disorder affecting small and medium sized arteries with unknown etiology and strong association with smoking. It is a difficult disease to treat and abstinence from smoking is must to arrest the progress. Conservative treatments like vasodilators, anti- coagulants, prostaglandin therapy etc. have questionable role. Arterial reconstructive surgery is not feasible usually. Sympathectomy, though widely used works partially or not at all. Progression of the disease invariably leads to amputation. Over the years, the methods and devices have evolved and its indications have been extended to treat fractures and associated complications: nonunion, chronic osteomyelitis, shortened extremity, joint contracture, and
Whooping cough is a common disease, especially in children that causes terrible and severe coughing (which is the reason for the name “whooping cough). It is a bacterial infection that is highly contagious.
Bordetella pertussis is one of and the most severe causative bacterium of whooping cough, the other being Bordetella parapertussis1. It is highly contagious and treatment is generally more effective if started earlier. However, it takes far too long to culture, and since the patient will not be isolated or treated until a diagnosis from the culture is made, this gives the bacteria more chances of infecting another person and further compromising the patient’s condition. Multiplex polymerase chain reaction (PCR) is a rapid, specific and sensitive molecular technique that will greatly improve patient care by providing a faster diagnosis. Although it does have a few downsides, the advantages it brings in the long run will far outweigh any disadvantages.
Imagine a hacking cough that sounds so hoarse that it would be scary for an adult, but with from a child. A child’s health is the most important thing to most parents and when something comes up that isn’t in a parent’s control it’s terrifying. There are a lot of diseases that just appear but there aren’t quite as many that only children are susceptible to. Laryngotracheobronchitis is one of the rare diseases that only gets caught by children and it’s important to be aware of what it is, the symptoms of it, and how to take care of it.