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Recurrent Incidence Of Babesiosis Among Adults Essay

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Recurrent incidence of babesiosis among apparently healthy bovines in Egypt, submits the needs for suitably destined prevalence studies with rapid and accurate diagnosis and immediate treatment of infected animals are required to control this disease. The routine epidemiological survey and diagnosis usually based upon microscopical examination of Giemsa stained blood smears. This technique is insufficient for accurate detection and identification of Babesia species during mixed infections and not reliable for detecting the infection in carrier cases or subclinical infections with low parasitemia. Combination of molecular and serological diagnostic tools for the infection provides powerful tools for accurate diagnosis as well as for epidemiological investigations as means of overcoming limitations of microscopical examination. The polymerase chain reaction will be conducted using highly conserved and species specific genes for detecting the parasite DNA in the blood of infected animals based on the B. bigemina rhoptry associated proteins, Apical membrane protein 1 & Internal trancribed spacer and B. bovis Spherical body proteins 4. The serological diagnostic tools, including the indirect fluorescent antibody test, the Indirect enzyme-linked immunosorbent assay, a competitive ELISA will be conducted to detect Babesia antibodies in serum of naturally infected cattle based on Babesia lysate, Cyanogen bromide-activated Sepharose 4B, affinity purified antigen and expressed,

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