Runx2 Binding Protein and the Regulation of Osteogenesis

1786 Words Jun 17th, 2018 8 Pages
Runx2 Binding Protein and the Regulation of Osteogenesis

In the developmental process of osteogenesis, bone is formed, laid down, and repaired in a highly regulated process (Wu et al., 2014a). This organized formation of bone tissue is controlled by the nucleic acid binding protein Runx2 (Wu et al., 2014a). Runx2 regulates transcriptional mechanisms in osteoblast cells, or bone forming cells, that are vital to the formation of bone tissue and to the maintenance of bone mass (Wu et al., 2014a). Osteoblasts phenotypically express certain genes depending upon the differentiation process they are regulated to undergo (Wu et al., 2014a). The commitment of osteoblasts to a particular stage-specific phenotype is dependent upon the expression
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Studying the interactions of Runx2 and RNAPII with promoters, the experiment demonstrated that 9–10% of genes in the human genome may be regulated by Runx2 in osteosarcoma cells (van der Deen et al., 2012). Runx2 is bound to over 2,000 genes that are actively transcribed based on co-interactions with polymerase (van der Deen et al., 2012). Data also demonstrated that Runx2 interacts with inactive genes that lacked RNAPII (van der Deen et al., 2012). These results confirmed hypothesis that Runx2 is a bi-functional regulator, activating or repressing transcription (van der Deen et al., 2012). An interesting result of this study involved Runx2’s influence on genes involving cell adhesion and motility (van der Deen et al., 2012). Combining data from the immunprecipation and gene expression profiles with siRNA, researchers concluded target genes can be both up-regulated and down-regulated with Runx2 depletion (van der Deen et al., 2012). This result also supports Runx2’s role as a bi-functional regulator of expression in these cells. Depletion of Runx2 decreased motility of U2OS cells (van der Deen et al., 2012). After identifying Runx2 target genes involved in motility, one can concluded depletion of Runx2 lowers cell motility in osteosarcoma cells (van der Deen et al., 2012). All in all, Runx2
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