Small Molecule Modulation of Voltage Gated Sodium Channels
Vincenzo Carnevale and Michael L. Klein
Institute for Computational Molecular Science, Department of Chemistry, Temple University, Philadelphia, PA 19122
Abstract
Voltage gated sodium channels are fundamental players in animals physiology. By triggering the depolarization of the lipid membrane they enable generation and propagation of the action potential. The involvement of these channels in numerous pathological conditions makes them relevant target for pharmaceutical intervention. Therefore, modulation of sodium conductance via small molecule binding constitutes a promising strategy to treat a large variety of diseases. However, this approach entails significant challenges: voltage gated sodium channels are complex nanomachines and the details of their workings have only recently started to become clear. Here we review ¬¬– with emphasis on the computational studies – some of the major milestones in the long-standing search of a quantitative microscopic description of the molecular mechanism and modulation of voltage-gated sodium channels.
Physiological Role of Voltage Gated Sodium Channels (VGSCs)
To respond to changes in the external environment, cells propagate electrical signals generated by transient, highly controlled transmembrane ionic currents.
Responsible for this process are ion channels, ubiquitous proteins that reside in membranes of excitable cells and convert chemical and electrical stimuli
A voltage-gated sodium ion channel opens when there is a change in the voltage of the membrane and allows sodium ions to flow across its electrochemical gradient. These voltage-gated channels are made up of amino acids and they aid in generating and moving an action potential down a membrane or axon (Brooker, Robert, 106).
At the molecular level of explanation these processes are dependent on the interplay between glutamate receptors, Ca2+ channels, the increase of intracellular Ca2+ levels, Ca2+-dependent proteins like Akt, ERK, mTOR and neurotrophins such as brain derived neurotrophic factor (BDNF) (24, 25).
Membranes can allow or exclude various molecules, and because of selective transport systems (active mediated transport), they can move molecules in and out of the space. Membrane channels, or “gates,” can open and close depending on the circumstances of the first messenger. Binding of an extracellular messenger to a dual receptor/channel brings about a quick
As well as these there are also the axon of the cell which is covered in myelin sheaths which carried information away from the cell body and hands the action potentials, these are small short bursts of change in the electrical charge of the axon membrane through openings of ion channels, off to the following neurons dendrites through terminal buttons at the end of the axons. Whenever an action potential is passed through these terminal buttons it releases a chemicals that pass on the action potential on to the next neuron through the terminal button and dendrite connection. The chemicals that are
To send a message, a neuron will send a ripple of electrical energy down its axon. This ripple is called "action potential." The way it works is by changing the chemical makeup of the axon's negatively charged interior. Positively charged sodium ions move into the cell and negatively charged potassium ions move out, then the ions move to their original positions. This produces a wave of positively charged
This stage is called repolarisation. The K+ channels then close, the sodium-potassium pump restarts, restoring the normal distribution of ions either side of the cell surface membrane and thus restoring the resting potential. In response to this the Na+ channels in that area would open up, allowing Na+ ions to flood into the cell and thus reducing the resting potential of the cells. If the resting potential of the cell drops to the threshold level, then an action potential has been generated and an impulse will be fired.
Once a presynaptic neuron is passive, an electrical current is spread along the length of the axon (Schiff, 2012). This is known as action potential (Pinel, 2011). Action potential happens once an abundant amount of depolarisation reaches the limit through the entry of sodium, by means of voltage gated sodium channels
Lidocaine binds to voltage-gated sodium channels, and prevents the flow of sodium ions. TTX irreversibly blocks voltage-gated sodium channels.
This experiment seeks to analyze how the resting membrane potential of Orconectes rusticus muscle cells changes in response to increasing [K+]o solution concentrations. By recording the intracellular voltage of the DEM, DEL1, and DEL2 crayfish muscle cells at six concentrations of [K+]o solution, we determined whether the observed resting membrane potentials (Vrest) were significantly different from the predicted Nernst equilibrium potential values. We hypothesized that the Vrest of the crayfish muscles at each concentration would not significantly differ from the Nernst potential, which solely considers the permeability of potassium ions to the cell membrane. However, our findings suggested differently, and results indicated that the Nernst equation did not accurately predict the obtained values of the resting membrane potential. The differences in muscle cell Vrest reveal instead that the membrane is differentially permeable to other ions.
Voltage gated channels are necessary components of life processes, in many organisms. One in particular, is the calcium voltage gated ion channel. Often lodged within the phospholipid bilayer, the imbalance of the calcium, or, the inside vs outside concentration, creates a gradient. The channel proteins often undergo conformations, states that which allow or block calcium ions from passing through. As ions move inside the cell, this creates a depolarization, or surge in the voltage. Clinically, this is associated with the heart and how it allows the heart to contract, which can be read in the
A nerve cell has a negative charge at a resting state due to negatively charged proteins within the cell.[3] Although the inside of the cell contains positively charged potassium ions as well, overall the charge is still negative. Along with potassium on the inside of the cell, positively charged sodium ions are located around the exterior of the cell.[3] When an action potential occurs, the cell becomes even more negatively charged. In turn, this causes sodium transport molecules in the membrane of the cell to open.[3] Sodium will then enter the cell during active transport. The positively charged sodium will cancel out the negatively charged active potential which will depolarize the cell. This allows neurotransmitters to transfer from cell to cell.[3] These neurotransmitters are what allows the body to feel pain. Local anesthetics work by diffusing through nerve fibers. Once they’ve reached the cells, they block the sodium transport molecules in the cell.[2] Therefore neurotransmitters cannot transfer information from cell to cell and the feeling of
The most frequent inflammatory disease of the central nervous system (CNS) impacts the lives of two and half million people in the world, Multiple Sclerosis (MS) (Schattling, 2013). Growing up, this disease has personally affected my family, and seeing a first hand account of the burden and turmoil that this disease causes for all of its patients it is critical to understand how this disease degenerates neurons and axons. The key players in this process are nervous system ion channels that regulate the influx and efflux of sodium and calcium, whether through exchangers or voltage-gated channels. There are normal molecular settings in neurons and there are MS molecular settings; the two are very different and progressively become further
Impulses will travel along the neuron pathways as the electrical charges move across each neural membrane. Ions that are moving across the membrane can cause the impulse to move along the nerve cells.
Depolarization in membrane potential triggers an action potential because nearby axonal membranes will be depolarized to values near or above threshold voltage.
Hence, this would allow for an influx of sodium into the cell down its electrochemical gradient. It would also allow for the flow of potassium outward, as it has a 140mM concentration inside the cell and wants to shift down its concentration gradient to 5.4mM. Therefore, this great driving force for the influx of sodium and efflux of potassium helps to explain the findings at this point. As Table 1 shows, the findings within this figure are statistically supported. The fact that there is not significant difference between the findings of this experiment and the calculated Nernst at the 10, 20 and 40mM of potassium is an indicator that sodium is the largest determinant of the resting membrane potential. However, some findings defy the expectations, as the last two concentrations elicit a resting membrane potential significantly more negative than expected. This can be explained by the fact that at this point, each muscle at their respective muscle groups has been protruded many