Spinach Lab Report

In this experiment, 4 grams of peeled turnip was used to prepare the enzyme extract opposed to the 1 gram of turnip suggested by Fundamentals of Life Science. Along with the change to the amount of turnip used, the amount of 0.1M phosphate buffer used to prepare the enzyme extract was changed from 50mL to 30mL. The affect of temperature on enzyme activity was not

The spinach plants were hole punched, mixed with 0.2% NaOH and dish detergent, placed in a syringe with the solution to have the oxygen

We used to florets from the cauliflower and disrupted the cell walls using a cold isolation buffer (0.3 M D-mannitol, 0.02 M phosphate buffer, pH 7.2) and an abrasive, we then strained the homogenate with cheesecloth into a centrifuge tube suppoerted by ice. The homogenate was then centrifuged at 600 g for 10 minutes at 4⁰C. The postnuclear supernatant was removed and centrifuged at 12,000 g for 30 minutes at 4⁰C. The post mitochondrial supernatant was removed and the mitochondrial pellet was resuspended in a cold assay buffer (0.3 M D-mannitol, 0.01 M KCl, 0.005 M MgCl₂, 0.02 M phosphate buffer, pH 7.2). Both samples were stored at

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

My lab partners and I performed an experiment that involved placing spinach disks into separate cups of distilled water (dH2O) and 0.2% sodium bicarbonate (NaHCO3) solution to examine photosynthesis in leaf tissue (Department of EEB, 2015). Discovering that the spinach disks quickly floated to the top of the 0.2% NaHCO3 solution and not in dH2O, we wondered if varied concentrations of carbonation would affect the rate of photosynthesis (PS). We tested this by halving the 0.2% NaHCO3 solution (using equal parts dH2O and 0.2% NaHCO3 solution to make 0.1% NaHCO3 solution). I hypothesize that if the spinach disks are placed in the 0.1% NaHCO3 solution, then they will have a slower PS compared to the disks placed in 0.2% NaHCO3. CO2

In generalization, there are a multitude of factors that could potentially influence the germination of a radish seed. This lab thoroughly exhibits the effect of water amounts on the germination of a radish seed. There is indeed an in-depth science behind the projected results, and overall of the effects water has on the germination of radish seeds, and the growth of plants in particular. Radishes themselves are moisture-loving plants; therefore, it is significant that they receive an adequate amount of water, allowing the soil to be moist, but not overly saturated (Biology Coach, 2015). In general, water is significant for the health of a plant in the way it transports important nutrients throughout the plant. From this point, nutrients are drawn from the soil and used by the plant. Seed germination itself is defined as the process where the seed sprout for growing, and future development into a plant. In order to germinate, the seed must have its essential needs met until it is capable of doing so: water, temperature, and sun. Therefore, during its early stages of growth, the seed will rely upon the food supplies stores within it, until it is large enough for its own leaves to begin making food through photosynthesis (Biology of Plants, 2016). Initially, the process of germination begins with the absorption of water y there seed, therefore, this absorption of water then activates an enzyme that increases

A plant's growth ability is dependent on its ability to acquire the resources it needs to survive. Competition such as interspecific and intraspecific, limiting resources, and population density affect the fitness level of a plant. This experiment was conducted in order to test the capability of collards and radishes to grow in manipulated densities under interspecific and intraspecific competition. I hypothesized that both collard and radish plants will grow more efficiently in single species pots under low-density conditions. I also hypothesized that in the mixed species plots the radishes will be more fit to survive and grow better than the collard plants in both the high and low-density pots. Both high and low density and single and mixed species plots were planted and results were observed. There was a significant

The polarity of each of the components of the spinach depends on the structure and the functional groups

In order to conduct this experiment a Spinacia oleracea was obtained, and measured using a plastic grid and a marker to outline the shape of the leaf. The squares of the grid were then counted to determine the total surface area of the leaf. The leaf was then placed vertically, and carefully, so that the underside of the Spinacia oleracea did not touch the walls of the micro-centrifuge tube when placed inside of it. Then the tube was filled ¾ full of water and 2ml full of the saturated sodium bicarbonate solution, using a plastic pipette.

Starting off the experiment, we turned on the spectrophotometer and set it to 605 nm. We created our chloroplast solutions using one solution with iceberg lettuce, one with spinach and one with kale. To create the solutions we started out by deveining the iceberg lettuce and placing it under a lamp. Then we put it in a chilled blender, added 0.5 M of chilled sucrose, and put it into the blender. After placing the lid on the blender, we blended the solution in three 10-second bursts.

This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.

1. Lab reports are to be computer-generated and double-spaced. All sections of the report must

Fill the container with a good-quality potting mix or garden soil mixed with compost or decomposed manure, because spinach requires a nutrient rich soil. Be sure your container has drainage holes in the bottom, and place the container on a water-catchment tray.

The purpose of this experiment was to take spinach leaves and extract the chlorophyll and carotenoid pigments by using acetone as the solvent. The chlorophyll and carotenoid pigments were extracted by using column chromography and alumina was used as the solvent. Solvents of different polarities were used, starting with the least polar, to extract the certain components from the leaves. They were then analyzed by using thin- layer chromatography.

First, we collected spinach leaves and cut out 60 disks using cork borer and then placed them in a syringe. Then, we added sodium bicarbonate into the syringe leaving about a third of the syringe empty and replace the plunger to the syringe. We aspirated the leaves by pulling the plunger down