Testing New Mycobacteriophage Genomes From Soil Or Water Samples

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ABSTRACT: Through experimentation new mycobacteriophage genomes were found and added to the scien-tific database. The objective was to find and isolate bacteriophage from soil or water samples, these samples could be taken from anywhere locally. Once the samples were purified through multiple steps, a method of electron microscopy was performed to isolate the viruses’ DNA. The soil sample we found was kept and that phage was infected with a host, m. smegmatis bacterium. Mycobacteriophage or phages are viruses that in-fect mycobacteria. The importance of this research is to find alternatives in the fields of genetics, ther-apeutics, and epidemiology ("Mycobacterium Smegmatis"). According to the SEA PHAGES Labor-atory Manual, phages are of interest for reasons such as replacing antibiotics, traditional therapies, and for using the phage DNA as a tool for further information. Phages are no susceptible to antibiot-ics, can survive in almost all types of environment, and are the most abundant life-form on earth ("Mycobacterium Smegmatis"). The research we are doing in this course is significant for we are discov-ering and sequencing a new phage in the matter of one semester.
Thus we will be characterizing a new phage that no one previously has discovered which contains new genes and data. This data will then be used by oth-er scientists to find information such as new thera-peutics, information regarding genes that cause disease, and molecular/biological evolution. Cur-rently

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