Girard Immanuel Soriano
Ecological Principles Lab
9/25/15
Lab Group: I
The Effect of C02 Concentration on the Photosynthetic Rate of Algae
Introduction:
How does the C02 concentration of algal solution affect the photosynthetic rate of Algae? In this experiment, algae solutions of different concentrations of carbon dioxide, on algal growth are tested to determine the effect of varying concentrations of carbon dioxide, on algal growth over a period of nine days. The rate of photosynthesis was deduced from the change in turbidity levels of the solution. There are three factors that affects the rate of photosynthesis: light intensity, temperature and carbon dioxide concentration. Photosynthesis involves several processes, two of them
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Variables:
The independent variable in this investigation is the carbon dioxide level in the algal solution. This is measured by determining the pH level of the solution as carbon dioxide gas is bubbled through the solution. When mixed, carbon dioxide and water form carbonic acid, thus decreasing the pH level of the solution.
The test cases five different algal solutions of different carbon dioxide levels measured in terms of pH value. For the 5 cases, the pH levels vary, starting with a control group without any carbon dioxide dissolved in the solution, and four other solutions that whose pH decreased by an increment of around 0.5 pH value from the control group.
The dependent variable in the investigation was the 9 day algal growth in each solution. The growth of algae is gauged by the change in the turbidity of the algal solutions from the initial and final day of data collection. Turbidity is a measure of the loss of water transparency due to the presence of particles in the water. One cause of turbidity is the presence of algae in water. In this experiment, the main cause of the increase in turbidity is the increase
Abstract: Photosynthesis, the conversion of inorganic carbon into organic glucose molecules using light energy, is one of the most biologically important processes on Earth. It is imperative to study how the rapidly increasing carbon dioxide concentrations in the atmosphere since the Industrial Revolution may affect photosynthesis of photoautotrophs. In this experiment, a look is taken at the question: does inorganic carbon availability affect photosynthetic activity. This experiment uses bicarbonate as the inorganic carbon source, and analyzes how varying concentrations of bicarbonate may affect the photosynthetic activity of the South American aquatic plant Egeria densa (also known as Elodea densa) by measuring its O2 production in distilled water and 0.1%, 0.4%, 0.6%, 0.8%, and 1.0% sodium bicarbonate solutions. T-tests between the control (distilled water) and each bicarbonate treatment are conducted using the online program GraphPad. All tests results in a p-value greater than 0.05 and a calculated t-value greater than the critical t-value, thus rejecting the null hypothesis, indicating that inorganic
We placed 50 mL of vinegar, tap water, seawater into three respective beakers. 50 mL of seltzer water was placed into a jar with a lid
I filled three clear cups, the first one with dH2O, the second with 0.1% NaHCO3 solution (equal parts 0.2% NaHCO3 and dH2O), and the third with 0.2% NaHCO3 solution. The control of the experiment is the cup with dH2O. The independent variable is time and the dependent variable is the number of disks floating in the solution. We separated the 30 disks into three groups of 10, placed them in syringes filled with a corresponding solution (either dH2O, 0.1% NaHCO3, or 0.2% NaHCO3), and removed all air from the syringe. This causes photosynthesis to stop in the disks, which then causes the disks to not float any longer. The three groups of disks were placed in each cup filled with 100mL corresponding to the solution, then placed under a light source and started a timer. For each minute in 15 minutes, data regarding the number of disks that floated to the top of each
This lab is used to test the effect the respiration rates in plants in animals and how it affects the level of carbon dioxide present in the water. Oxygen and carbon dioxide are gases that are vital to all organisms, whether it is given or released through that organism. Both plants and animals use oxygen and carbon dioxide for cellular respiration, giving off carbon dioxide as a waste product. This lab is an example of cellular respiration in both plants and animals. The change in the carbon dioxide levels will be
The rate of photosynthesis may vary with changes that occur in environmental temperature, wavelength of light, and light intensity. Using a photosynthetic organism of your choice, choose only ONE of the three variables (temperature, wavelength of light, or light intensity) and for this variable.
When using algae beads and a CO2 indicator, the process of Photosynthesis and Cellular Respiration can be measured. In this experiment the intensity of light will be altered in each trail, and the rate of Photosynthesis will then be measured. As you rise from low light intensity to higher light intensity, the rate of photosynthesis will increase because there is more light available to drive the reactions of photosynthesis. However, once the light intensity gets high enough, the rate won’t increase anymore since there will be more-light than water and CO2; there will not be enough components from light, water, and CO2 to create the process of Photosynthesis. As CO2 dissolves and the amount of CO2 goes up, the pH will lower, which means the solutions color will change varying form red, orange and yellow, all pending on what the pH is at. CO2 will be produced from respiration, all while photosynthesis absorbs the CO2. This means that when the rate of photosynthesis is less than respiration, pH levels will decrease, and CO2 concentration will increase. Vis versa, when pH levels increase
The results of the mean of the concentrations are as followed; 0%, 0.2%, 2.0% and 1.0% which are in organization of longest float time to shortest. The higher the concentration of sodium bicarbonate (carbon source) the quicker the process of photosynthesis occurs. The mean of the concentrations is; 0% is 900, 0.20% is 757.58, 1.0% is 580.004 and 2.0% is 582.54. The standard deviations of the concentrations are; 0% is 0, 0.20% is 190.0045, 1.0% is 247.9306 and 2.0% is 199.979.
The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related
When there are excessive loads of Nitrogen and Phosphorous in the water, Alage can “bloom” to harmful levels, changing water color, and eventually stripping dissolved oxygen from the water when they die, fall to the bottom, and decay. This dissolved oxygen is critical to the health of the Chesapeake Bay’s critters and
The amount of light can change the number of the bubble which is the air that the photosynthesis product, it increase the speed to produce the air. when the intensity of light is high, you will get a greater rate of photosynthesis. Light intensity is usually defined as the energy hitting an area over some time period. At low light intensities and when light intensity increases, the rate of the light-dependent reaction, and therefore photosynthesis generally, increases proportionately. The more photons of light that fall on a leaf, the greater the number of chlorophyll molecules that are ionized and the more ATP and NADPH are generated. As light intensity increases, the rate of photosynthesis will increase as long as other factors are inadequate
The purpose of this experiment is to understand the effects of nutrient enrichment and eutrophication, using samples of water from Rio Salado and Encanto Park. The samples will contain different concentration levels of nitrogen, phosphorous and nitrogen and phosphorous combined and the impact it has on algae growth. The results recorded showed that the nitrogen concentration levels had a little change, phosphorous levels had a higher change and phosphorous and nitrogen combined had a significantly higher change, resulting in higher algae growth. The results showed that phosphorous indeed is a limiting nutrient in algae growth, but to achieve the highest growth rate, both nitrogen and phosphorous need to be combined.
The intention of this experiment is to determine the effects of pH on the rate of photosynthesis in living leaves. Photosynthesis is a process by which plants convert light energy captured from the sun into chemical energy which they use to perform various plant functions. During the photosynthesis process, light, carbon dioxide, and water react to produce products: sugar and oxygen. The equation for photosynthesis is:
The algae growth shown in figure 1 has no clear connection due to the rapid fluctuations. With this being said, the 4 and 40 degree linear trends show a steady decrease in algae over the 13 day period while the 23 degree linear trend climbed steadily. Discussion Both table 1 and figure 1 shows that the
For part B, 50 mL of an assigned 50 mL pH solution of either 1 M HCl, 1 M NaOH, lemon juice, and 50 mL of household bleach all in separate 250 mL beakers are to be used. For part C, a hot plate or ice are to be used to make the 1.0 mL assigned temperature specific water. This experiment will also use the 1.0 mL of 0.1 Phosphate buffer.
This lab will be driven by the research question, what is the effect of temperature (at 5oC, 15oC, 25oC, 35oC and 45oC) on the photosynthetic rate (measure of oxygen bubbles over a period of time) of Elodea (pondweed)?