The Effect Of Temperature On Enzyme Activity

936 WordsFeb 4, 20164 Pages
How Temperature and pH Affect Enzyme Activity In this lab, we had learned how both temperature and pH affect the enzyme activity. We created a hypothesis and later tested them using 4 procedures. These procedures included test tubes, cuvettes, baths with different temperatures, thermometers, chemicals and spectrophotometers. We had created graphs to show a visual of the data we had collected rather than just simply showing numbers. Having a graph was very helpful, so we can better compare the data. This lab had helped us better understand enzymes. Enzymes are a vital component for all living things, it is needed for photosynthesis, hydrolysis, respiration, digestion, and as well as dehydration synthesis. Enzymes are organic catalysts, which means that they begin or accelerate any kind of reaction without being depleted or changed eternally in the process. Enzymes have differing methods but still all produce catalysis by lowering the activation energy for the reaction, which allows it to occur more smoothly. Alteration of temperature can critically modify enzyme catalysis. For example, as the temperature rises the enzyme activity will also increase and will peak at 37-38C. As temperature goes down excessive heat causes the alteration of its tertiary structure which is known as denaturation. Both pH and temperature can immensely impact the tertiary structure of proteins, therefore enzyme because enzymes are proteins. pH may not only affect the structure of an enzyme but as
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