investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills, the experiment was done in order to determine how the rate of reaction will be altered, whether it will increase, decrease or remain constant when the different concentration of enzymes added. INTRODUCTION: Enzymes are produced naturally in plant, animal, and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe
investigate the effect of enzyme concentration on the rate of reaction PREDICTION: An enzyme is a biological catalyst. They speed up the rate of a reaction however they do not affected themselves whilst doing this, which is why they are catalysts. Enzymes are made to be specific, this means that they can have only one substrate that they will work on. Each enzyme has an active site that is where their own specific substrate’s molecule will fit into. The enzymes catalyze chemical reactions beginning
Enzyme concentration has a direct impact on the rate of reaction. When looking at graph 1 it is easy to see a linear relationship between the rate of formation of NADH and the concentration of the enzyme. As enzyme concentration increases the rate of reaction increases because substrates are more likely to collide with available enzymes. Le Chatelier's principle supports this as adding more enzyme will cause the reaction to shift more towards the enzyme substrate complex which will result in the
explore the effects of enzyme concentration, substrate concentration, temperature, and inhibitors on reaction rate, respectively. To test each of these factors, four activities were completed. The enzyme from Turnip Extract and the substrate Peroxide were tested. The turnip extract was tested at the following concentrations: .5ml, 1.0ml, and 2.0ml. Peroxide was tested at the following concentrations: 0.1ml, 0.2ml, and 0.4ml. In order to understand the effect of temperature on reaction rate the following
The Effects Temperature, pH and enzyme concentration have on Enzyme Catalase Tiffany Sievers Biology 110, Community College of Baltimore County Abstract The purpose of this experiment was to test the effects that temperature, pH, and substrate concentration have on an enzyme catalase reaction. What affects will temperature, substrate concentration, and pH levels have on an enzymes catalase reaction? Temperature, pH levels and substrate concentration will have an effect on the
TITLE: ENZYME CATALYSIS PURPOSE: Measure the effects of changes in catalase concentration, substrate concentration, and salinity on the reaction rates of an enzyme. RESEARCH COMPONENTS: What is being tested-what do you know about enzymes? PRELAB QUESTIONS: Explain how the structure of an enzyme is related to its function/activity. All enzymes are structured to react with unique substrates. and each enzyme has an active site where the substrate bonds to the enzyme. The active site of an enzyme
measure the activity of the enzyme, catechol oxidase. Specifically, they investigate the effect of decreasing amounts of enzyme on rate of reaction and effect of decreasing amounts of substrate on rate of reaction. Enzymes are proteins with specific structures determined by the sequence of amino acid used to accelerate and regulate biochemical processes; enzyme activity can be measured using the rate at which the reaction catalyzes and can be expressed in concentration of substrate or product. Catechol
Effects of Temperature, pH, Enzyme Concentration, and Substrate Concentration on Enzymatic Activity INTRODUCTION Enzymes, proteins that act as catalysts, are the most important type of protein[1]. Catalysts speed up chemical reactions and can go without being used up or changed [3] Without enzymes, the biochemical reactions that take place will react too slowly to keep up with the metabolic needs and the life functions of organisms. Catecholase is a reaction between oxygen and catechol
Enzyme Properties under Altered Conditions Melissa Piccione N01696299 BIOL 1500 Instructor: Dr. Christopher Blanar Date: 7 October 2015 Abstract: An important part of the function of enzymes is dependent on their structure. Factors that affect this functionality are temperature, concentration, and pH. The effects of pH, enzyme concentration, and temperature on the catalytic rate of various enzymes were explored through three controlled laboratory experiments on alkaline phosphatase’s (ALP)
1.0 Introduction With the constant integration of technology into human households, it is apparent that biological enzymes such as the alpha amylase are to be implemented into sanitary procedures. One such example lies within the utilisation of enzymes in laundry detergents. Enzymes have recently assisted the development and improvement of modern household and industrial detergents (Journal of surfactants and detergents 1998). The alpha amylase is essential in hydrolysis of starch molecules, as