The Effect of Enzyme Concentration on Rate of Reaction

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OBJECTIVE:

The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills, the experiment was done in order to determine how the rate of reaction will be altered, whether it will increase, decrease or remain constant when the different concentration of enzymes added.

INTRODUCTION:

Enzymes are produced naturally in plant, animal, and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules that are greatly increase the rate of chemical reaction without themselves being changed at the end of the reaction. A chemical reaction involves the conversion of a
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Genuinely, it is a very pale blue liquid. It is slightly more viscous than water. Hydrogen peroxide is a weak acid but it is a strong oxidizing agent that acts as a bleaching agent, disinfectant, oxidizer, antiseptic and the like. It is produced as a byproduct of oxygen metabolism and by all organisms that possessed enzyme peroxidase. Hydrogen peroxide always decomposes exothermically into water and oxygen gas spontaneously. The rate of decomposition is dependent on the temperature and concentration of the peroxide, as well as the pH and the presence of impurities and stabilizers.
Hydrogen peroxide is incompatible with many substances that catalyse its decomposition, including most of the transition metals and their compounds. Common catalysts include manganese dioxide and silver apart from enzyme catalase as has been used in this experiment. A common concentration for hydrogen peroxide is "20 volumes", which means that when 1 volume of hydrogen peroxide is decomposed, it produces 20 volumes of oxygen. A 20 "volume" concentration of hydrogen peroxide is equivalent to 1.67 mol/dm3 (Molar solution) or about 6%.Hydrogen peroxide available at drug stores is three percent solution. In such small concentrations, it is less stable, and decomposes faster. It is usually stabilized with acetanilide, a substance which has toxic side effects in significant amounts.
Buffer solution is used in this experiment as to keep the pH at a
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