OBJECTIVE:
The experiment was carried out to investigate the effects of the increase in the enzyme concentration on the rate of reaction. By using self investigative and experimental skills, the experiment was done in order to determine how the rate of reaction will be altered, whether it will increase, decrease or remain constant when the different concentration of enzymes added.
INTRODUCTION:
Enzymes are produced naturally in plant, animal, and microbial cell. There are thousands of different enzyme can be found in any cell. Enzymes can be describe as molecules that are greatly increase the rate of chemical reaction without themselves being changed at the end of the reaction. A chemical reaction involves the conversion of a
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Genuinely, it is a very pale blue liquid. It is slightly more viscous than water. Hydrogen peroxide is a weak acid but it is a strong oxidizing agent that acts as a bleaching agent, disinfectant, oxidizer, antiseptic and the like. It is produced as a byproduct of oxygen metabolism and by all organisms that possessed enzyme peroxidase. Hydrogen peroxide always decomposes exothermically into water and oxygen gas spontaneously. The rate of decomposition is dependent on the temperature and concentration of the peroxide, as well as the pH and the presence of impurities and stabilizers.
Hydrogen peroxide is incompatible with many substances that catalyse its decomposition, including most of the transition metals and their compounds. Common catalysts include manganese dioxide and silver apart from enzyme catalase as has been used in this experiment. A common concentration for hydrogen peroxide is "20 volumes", which means that when 1 volume of hydrogen peroxide is decomposed, it produces 20 volumes of oxygen. A 20 "volume" concentration of hydrogen peroxide is equivalent to 1.67 mol/dm3 (Molar solution) or about 6%.Hydrogen peroxide available at drug stores is three percent solution. In such small concentrations, it is less stable, and decomposes faster. It is usually stabilized with acetanilide, a substance which has toxic side effects in significant amounts.
Buffer solution is used in this experiment as to keep the pH at a
This process is called decomposition as two hydrogen peroxide molecules are broken down into oxygen and two water molecules.
Used to see if the temperature of the water is at 37oc – 40oc and if
Introduction:Enzymes are made up of proteins which are produced within living cells and act as catalysts which speed up chemical reactions. They are made up of long chains of amino acids containing carbon, hydrogen, oxygen and nitrogen. Enzymes are structured to be
Question: How does changing enzyme concentration or temperature affect the reaction time of enzyme activity?
PURPOSE: Measure the effects of changes in catalase concentration, substrate concentration, and salinity on the reaction rates of an enzyme.
The motive of this lab is to attain a better understanding of enzyme activity by timing chemical reactions in certain temperatures and pH levels. Enzymes act as catalysts that help speed up reactions. Without these enzymes chemical reactions in metabolism would be backed up. There are two factors that affect an enzyme’s reaction rate: temperature and pH levels. In this label we will be testing different pH levels and temperatures to see which ones cause the most reactions.
Substrate concentration also affects the rate of reaction as the greater the substrate concentration the faster the rate of reaction and all the active sites are filled. At this point the rate of reaction can only be increased if you add more enzymes in to make more active sites available.
In this experiment we tested the effects that enzymes and substrate have on chemical reaction rates, which is the rate at which chemical reactions occur.. This experiment tested how different concentrations of enzyme and substrate affected the light absorption measurements on a spectrophotometer. The experiment also tested how temperature affected the light absorption, and in a separate test, the effect of the enzyme inhibitor hydroxylamine was also tested. In the first test conducted, 3 different concentrations of enzyme, and three different concentrations of substrate were measured in a spectrophotometer. For the enzyme and the substrate, the measurements got higher as the concentrations were higher, but the over measurements of the substrate were smaller than those of the enzyme. In the second test conducted, the medium concentration enzyme was tested under the temperatures; 4°C, 23°C, 37°C, and 60°C. The measurements in this test got higher as the temperature got higher, but did the measurements under 4°C were overall significantly higher than the other temperature measurements. Lastly, the last test conducted showed that the measurements of the substance with 0 and 1 drop of hydroxylamine inhibitor went up, but the measurements of the enzyme with 5 drops of hydroxylamine inhibitor stayed rather low and did not change much. In conclusion, these experiments showed that chemical reaction rates are sped up with higher concentrations of enzyme, substrate,
Introduction Cells require the use of enzymes to carry on life functions. These functions include breaking down molecules for energy, building structural molecules such proteins and producing various chemical messengers. Because most reactions require high amounts of energy, they could not occur in a cell without the aid of a catalyst. Enzymes are protein catalysts that bind to molecules allowing them to react faster. This can occur without the high amount of energy the molecules would normally need.
Enzymes are high molecular weight molecules and are proteins in nature. Enzymes work as catalysts in biochemical reactions in living organisms. Enzyme Catecholase is found on in plants, animals as well as fungi and is responsible for the darkening of different fruits. In most cases enzymatic activities are influenced by a number of factors, among them is temperature, PH, enzyme concentration as well as substrate concentration (Silverthorn, 2004). In this experiment enzyme catecholase was used to investigate the effects of PH and enzyme concentration on it rate of reaction. A pH buffer was used to control the PH, potato juice was used as the substrate and water was used as a solvent.
Enzymes are the most important types of proteins, they act as catalysis (speed up chemical reactions). If enzymes didn’t exist, biochemical reactions would act to slowly and they couldn’t keep up with the metabolic functions. Enzymes have a three-dimensional structure that is really complex. This structure consists of one or more polypeptide chains, they form an active site, which is an area in which the substrate eventually will fit. The four factors that affect the activity and reaction rate of an enzyme are temperature, pH, enzyme concentration and
In the exercise # 2 we observed the effect of substrate concentration, enzyme concentration, pH and temperature on enzyme activity. All the data showed that once potato extract was added to catechol and water the reaction varied dependent on the level of catechol. As in
The effect of temperature and ph level of an enzyme activity was then tested. When temperature increases, the kinetic energy, energy at work, increases. At first, the increase of temperature will increase the rate of reaction because enzyme catalase reacts by colliding with substrate molecules. (Ottaway, J. H). The
The efficiency of enzymes depends on a number of factors, such as temperature, pH and the concentration of the enzyme just to name a few. This experiment was conducted to show the effects of different pH levels on the activity of the catalase enzyme, the data obtained in this experiment supports the initial hypothesis of the experiment which states that the catalase enzyme will function optimally at a pH of 7 and efficiency will decrease as the pH of solutions moves further away from 7. This is true because in the experiment the test tube containing the distilled water which has a pH of 7, produced the highest amount of foam out of the four test tubes. The efficiency of the reaction can be determined by the amount of foam (foam
The purpose of this lab report is to investigate the effect of substrate concentration on enzyme activity as tested with the enzyme catalase and the substrate hydrogen peroxide at several concentrations to produce oxygen. It was assumed that an increase in hydrogen peroxide concentration would decrease the amount of time the paper circle with the enzyme catalase present on it, sowing an increase in enzyme activity. Therefore it can be hypothesised that there would be an effect on catalase activity from the increase in hydrogen peroxide concentration measured in time for the paper circle to ride to the top of the solution.